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  • 99
    Thermo Fisher sybrgreen pcr master mix
    HPV <t>SybrGreen</t> <t>PCR</t> and differentials of association curves of PCR products. A: Real-time SybrGreen amplification patterns of four SiHa cell line dilutions corresponding to an input of 50, 25, 12, and 2 HPV genomes ( A ) and a selection of 6 of the 25 ovarian cancer samples. The four SiHa cell line dilutions and 3 of the 25 ovarian cancer cases passed the threshold ( B ). For reasons of clarity, only 5 of the 22 other ovarian cancer samples not passing the threshold are depicted ( C ). A (mock tissue) negative control ( D ) is also shown. B: The differentials of the association curves of the samples in A (identical labeling patterns). With two exceptions, the low-copy number HPV input sample and one of the three ovarian cancer samples that surpassed the SybrGreen threshold, the curves can be placed in three clusters: one with a peak around 80°C (representing fragmented high-molecular weight DNA that smears higher up on a gel), one with a peak around 73°C (representing HPV amplicons that give a distinct band on gel), and one with a peak around 63°C (representing fragmented low-molecular weight DNA and/or primer dimers that run low in the gel).
    Sybrgreen Pcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen pcr master mix/product/Thermo Fisher
    Average 99 stars, based on 1652 article reviews
    Price from $9.99 to $1999.99
    sybrgreen pcr master mix - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher sybrgreen pcr master kit
    Impact of nonspecific signal in <t>PCR</t> quantification read-outs. Individual cells expressing or not Gzmb mRNA were amplified simultaneously. ( A ) <t>SYBR</t> Green signal in positive (solid lines) and negative (dotted lines) cells. ( B ) C T evaluation, using the Sequence Detector 1.7 software. The C T value of negative cells and wells not containing template was not significantly different ( t -test: P = 0.18). This program's upper limit of detection is 60 cycles; that is, samples without template score with a C T of 60.
    Sybrgreen Pcr Master Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen pcr master kit/product/Thermo Fisher
    Average 99 stars, based on 13012 article reviews
    Price from $9.99 to $1999.99
    sybrgreen pcr master kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher sybrgreen kit
    Impact of nonspecific signal in <t>PCR</t> quantification read-outs. Individual cells expressing or not Gzmb mRNA were amplified simultaneously. ( A ) <t>SYBR</t> Green signal in positive (solid lines) and negative (dotted lines) cells. ( B ) C T evaluation, using the Sequence Detector 1.7 software. The C T value of negative cells and wells not containing template was not significantly different ( t -test: P = 0.18). This program's upper limit of detection is 60 cycles; that is, samples without template score with a C T of 60.
    Sybrgreen Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybrgreen kit/product/Thermo Fisher
    Average 99 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    sybrgreen kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    HPV SybrGreen PCR and differentials of association curves of PCR products. A: Real-time SybrGreen amplification patterns of four SiHa cell line dilutions corresponding to an input of 50, 25, 12, and 2 HPV genomes ( A ) and a selection of 6 of the 25 ovarian cancer samples. The four SiHa cell line dilutions and 3 of the 25 ovarian cancer cases passed the threshold ( B ). For reasons of clarity, only 5 of the 22 other ovarian cancer samples not passing the threshold are depicted ( C ). A (mock tissue) negative control ( D ) is also shown. B: The differentials of the association curves of the samples in A (identical labeling patterns). With two exceptions, the low-copy number HPV input sample and one of the three ovarian cancer samples that surpassed the SybrGreen threshold, the curves can be placed in three clusters: one with a peak around 80°C (representing fragmented high-molecular weight DNA that smears higher up on a gel), one with a peak around 73°C (representing HPV amplicons that give a distinct band on gel), and one with a peak around 63°C (representing fragmented low-molecular weight DNA and/or primer dimers that run low in the gel).

    Journal: The American Journal of Pathology

    Article Title: A Novel Strategy for Human Papillomavirus Detection and Genotyping with SybrGreen and Molecular Beacon Polymerase Chain Reaction

    doi:

    Figure Lengend Snippet: HPV SybrGreen PCR and differentials of association curves of PCR products. A: Real-time SybrGreen amplification patterns of four SiHa cell line dilutions corresponding to an input of 50, 25, 12, and 2 HPV genomes ( A ) and a selection of 6 of the 25 ovarian cancer samples. The four SiHa cell line dilutions and 3 of the 25 ovarian cancer cases passed the threshold ( B ). For reasons of clarity, only 5 of the 22 other ovarian cancer samples not passing the threshold are depicted ( C ). A (mock tissue) negative control ( D ) is also shown. B: The differentials of the association curves of the samples in A (identical labeling patterns). With two exceptions, the low-copy number HPV input sample and one of the three ovarian cancer samples that surpassed the SybrGreen threshold, the curves can be placed in three clusters: one with a peak around 80°C (representing fragmented high-molecular weight DNA that smears higher up on a gel), one with a peak around 73°C (representing HPV amplicons that give a distinct band on gel), and one with a peak around 63°C (representing fragmented low-molecular weight DNA and/or primer dimers that run low in the gel).

    Article Snippet: Amplification reactions were performed with 1× SybrGreen PCR Master Mix (Applied Biosystems), 200 nmol/L of CPII/G/CPI primers and 5 μl of sample DNA in a 50-μl final volume.

    Techniques: Polymerase Chain Reaction, Amplification, Selection, Negative Control, Labeling, Low Copy Number, Molecular Weight

    Comparison of real-time HPV PCR amplification reported by molecular beacons and SybrGreen. A selection of three samples from cervical cancer patients and a negative control (mock tissue section) is shown. HPV molecular beacon PCR ( filled symbols ): the three patient samples were genotyped as HPV-16 ( squares ), HPV-45 ( triangles ), and HPV-18 ( circles ). The mock tissue was negative with all seven HPV molecular beacons ( diamonds ). HPV SybrGreen PCR ( open symbols ): all three patient samples reported positive, whereas the negative control did not. The dashed line represents the threshold.

    Journal: The American Journal of Pathology

    Article Title: A Novel Strategy for Human Papillomavirus Detection and Genotyping with SybrGreen and Molecular Beacon Polymerase Chain Reaction

    doi:

    Figure Lengend Snippet: Comparison of real-time HPV PCR amplification reported by molecular beacons and SybrGreen. A selection of three samples from cervical cancer patients and a negative control (mock tissue section) is shown. HPV molecular beacon PCR ( filled symbols ): the three patient samples were genotyped as HPV-16 ( squares ), HPV-45 ( triangles ), and HPV-18 ( circles ). The mock tissue was negative with all seven HPV molecular beacons ( diamonds ). HPV SybrGreen PCR ( open symbols ): all three patient samples reported positive, whereas the negative control did not. The dashed line represents the threshold.

    Article Snippet: Amplification reactions were performed with 1× SybrGreen PCR Master Mix (Applied Biosystems), 200 nmol/L of CPII/G/CPI primers and 5 μl of sample DNA in a 50-μl final volume.

    Techniques: Polymerase Chain Reaction, Amplification, Selection, Negative Control

    Ct values of the β-globin molecular beacon PCR ( closed circles ) and the HPV-CPI/IIG-SybrGreen PCR ( open squares ) as a function of the amount of input SiHa cell DNA. SiHa carries one to two copies of HPV-16. Bars indicate standard errors of three independent experiments, the range of standard errors: 0.1 to 0.76 cycles. With the ΔCt approach accurate quantification of viral over nuclear genome copy number is possible even with 20 cell equivalents.

    Journal: The American Journal of Pathology

    Article Title: A Novel Strategy for Human Papillomavirus Detection and Genotyping with SybrGreen and Molecular Beacon Polymerase Chain Reaction

    doi:

    Figure Lengend Snippet: Ct values of the β-globin molecular beacon PCR ( closed circles ) and the HPV-CPI/IIG-SybrGreen PCR ( open squares ) as a function of the amount of input SiHa cell DNA. SiHa carries one to two copies of HPV-16. Bars indicate standard errors of three independent experiments, the range of standard errors: 0.1 to 0.76 cycles. With the ΔCt approach accurate quantification of viral over nuclear genome copy number is possible even with 20 cell equivalents.

    Article Snippet: Amplification reactions were performed with 1× SybrGreen PCR Master Mix (Applied Biosystems), 200 nmol/L of CPII/G/CPI primers and 5 μl of sample DNA in a 50-μl final volume.

    Techniques: Polymerase Chain Reaction

    Impact of nonspecific signal in PCR quantification read-outs. Individual cells expressing or not Gzmb mRNA were amplified simultaneously. ( A ) SYBR Green signal in positive (solid lines) and negative (dotted lines) cells. ( B ) C T evaluation, using the Sequence Detector 1.7 software. The C T value of negative cells and wells not containing template was not significantly different ( t -test: P = 0.18). This program's upper limit of detection is 60 cycles; that is, samples without template score with a C T of 60.

    Journal: Genome Research

    Article Title: Quantification of Multiple Gene Expression in Individual Cells

    doi: 10.1101/gr.2890204

    Figure Lengend Snippet: Impact of nonspecific signal in PCR quantification read-outs. Individual cells expressing or not Gzmb mRNA were amplified simultaneously. ( A ) SYBR Green signal in positive (solid lines) and negative (dotted lines) cells. ( B ) C T evaluation, using the Sequence Detector 1.7 software. The C T value of negative cells and wells not containing template was not significantly different ( t -test: P = 0.18). This program's upper limit of detection is 60 cycles; that is, samples without template score with a C T of 60.

    Article Snippet: Real-time quantitative PCR was performed by adding 10 μL of 2× SYBR Green PCR Master Mix (Applied Biosystems) to each well containing 4 μL of template and 6 μL of a primer mix with 0.25 μM of each specific primer (see Supplemental materials I and II) in a 20-μL reaction volume using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems).

    Techniques: Polymerase Chain Reaction, Expressing, Amplification, SYBR Green Assay, Sequencing, Software

    mRNA levels for articular cartilage markers (aggrecan, type II collagen (α1(II)) and catabolic markers (Cox-2, IL-6, iNOS, MMP-13) in vehicle-treated, Wnt3a-treated, IL-1ß-treated, or combined Wnt3a- and IL-1ß-treated human articular chondrocytes overexpressing AnxA6. Human articular chondrocytes were transfected with empty expression vector (EV) or expression vector containing full-length AnxA6 ( AnxA6 ). Twelve hours after transfection cells were serum-starved for 24 h followed by treatment with IL-1ß, Wnt3a, IL-1ß, or combined IL-1ß and Wnt3a for 24h. Levels of mRNA were determined by real-time PCR using SYBR Green and normalized to the level of 18S RNA. The mRNA levels are expressed relative to the level of vehicle-treated cells transfected with empty expression vector, which was set as 1. Data were obtained from triplicate PCRs using RNA from 3 different cultures ( n = 3). Values are the mean ± SD. (**p

    Journal: PLoS ONE

    Article Title: Annexin A6 regulates catabolic events in articular chondrocytes via the modulation of NF-κB and Wnt/ß-catenin signaling

    doi: 10.1371/journal.pone.0197690

    Figure Lengend Snippet: mRNA levels for articular cartilage markers (aggrecan, type II collagen (α1(II)) and catabolic markers (Cox-2, IL-6, iNOS, MMP-13) in vehicle-treated, Wnt3a-treated, IL-1ß-treated, or combined Wnt3a- and IL-1ß-treated human articular chondrocytes overexpressing AnxA6. Human articular chondrocytes were transfected with empty expression vector (EV) or expression vector containing full-length AnxA6 ( AnxA6 ). Twelve hours after transfection cells were serum-starved for 24 h followed by treatment with IL-1ß, Wnt3a, IL-1ß, or combined IL-1ß and Wnt3a for 24h. Levels of mRNA were determined by real-time PCR using SYBR Green and normalized to the level of 18S RNA. The mRNA levels are expressed relative to the level of vehicle-treated cells transfected with empty expression vector, which was set as 1. Data were obtained from triplicate PCRs using RNA from 3 different cultures ( n = 3). Values are the mean ± SD. (**p

    Article Snippet: PCRs were performed with a SYBR Green PCR Master Mix kit (Applied Biosystems), with 95°C for 10 min followed by 40 cycles at 95°C for 15 s and 60°C for 1 min, and 1 cycle at 95°C for 15 s and 60°C for 1 min.

    Techniques: Transfection, Expressing, Plasmid Preparation, Real-time Polymerase Chain Reaction, SYBR Green Assay