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Image Search Results
Journal: Cell reports
Article Title: Inflammatory adipose activates a nutritional immunity pathway leading to retinal dysfunction
doi: 10.1016/j.celrep.2022.110942
Figure Lengend Snippet: (A) Human fetal RPE (fRPE) cultures were treated with either transferrin-bound ferric iron (Tf· 55 Fe 3+ ) or ferrous iron ascorbate ( 55 Fe 2+ ) alone or in the presence of recombinant IL-1β (rIL-1β). Intracellular iron accumulation in fRPE lysates was measured during 8 h of treatment using a scintillation counter to quantify counts per minute (CPM) (n = 3/group). (B) fRPE cultures were treated with either media (vehicle) or recombinant IL-1β (rIL-1β) for 8 h before cells were harvested for qPCR analysis of iron transport/ regulatory genes (n = 6/group). (C) Mass in grams of WT and Il1b −/− (KO) mice fed an LFD or an HFD (n = 20/group). (D) VAT and SAT hypertrophy were dependent on diet but not genotype (n = 10/group). (E) Diet and genotype had no effect on glycemic control measured with intraperitoneal glucose tolerance test (n = 10/group). (F) RPE gene expression measured by qPCR following 12 weeks of either LFD or HFD (n = 5/group). (G) Retinal CD11b + cell gene expression following 12 weeks of either LFD or HFD (n = 5/group). (H) HFD WT mice accumulate RPE iron, measured using inductively coupled plasma mass spectrometry, which is rescued in the HFD Il1b −/− mice (n = 10/group). (I) HFD WT mice accumulate lipid oxidation product MDA in RPE, which is rescued in HFD Il1b −/− mice (n = 10/group). (J) HFD WT mice RPE upregulate Hmox1 in an Il1b -dependent manner (n = 5/group). (K) HFD WT mice exhibit c-wave amplitude deficits, measured by electroretinography, which is rescued in HFD Il1b −/− mice (n = 10/group). Data indicate mean and, where error bars are shown, ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, by 1-way ANOVA with multiple comparisons (A), unpaired Student’s t test (B), or 2-way repeated-measures ANOVA (C and E) or 2-way ANOVA (D and F–K) with Tukey’s HSD post hoc test.
Article Snippet: 100ul of the cell lysate was added to a scintillation tube with 1mL of
Techniques: Recombinant, Control, Gene Expression, Clinical Proteomics, Mass Spectrometry