Journal: The Journal of Experimental Medicine

Article Title: Innate Recognition of Bacteria in Human Milk Is Mediated by a Milk-Derived Highly Expressed Pattern Recognition Receptor, Soluble Cd14

doi:

Figure Lengend Snippet: IECs can be activated by LPS or whole bacteria by an m-sCD14–dependent mechanism. (A–D) IL-8, TNF-α, and ENA-78 production was tested by ELISA in supernatants of HT29 and SW620 cell lines cultured in medium supplemented with human milk (HM) and stimulated with E . coli LPS or varying numbers of whole E . coli (C and D; 2.5 × 10 6 E . coli ). The anti-CD14 mAb MY4 (IgG2b) but not its isotype-matched control blocked LPS and bacterial stimulation. Representative results with HT29 cells are shown.

Article Snippet: The human colon carcinoma epithelial cell lines HT29 and SW620, myeloid cell line U937 (American Type Culture Collection), astrocytoma line U373 MG, and breast adenocarcinoma cell line with characteristics of differentiated mammary epithelium, MCF-7 (European Collection of Animal Cell Cultures), were cultured as indicated by the purveyor.

Techniques: Bacteria, Enzyme-linked Immunosorbent Assay, Cell Culture, Control

Journal: OncoTargets and therapy

Article Title: miR-486-5p attenuates tumor growth and lymphangiogenesis by targeting neuropilin-2 in colorectal carcinoma

doi: 10.2147/OTT.S103460

Figure Lengend Snippet: NRP2 is a target of miR-486-5p. Notes: ( A ) Effect of miR-486-5p on NRP2 expression as determined by a luciferase reporter assay. The data were normalized by determining the firefly and Renilla luciferase activities measured at 72 hours posttransfection; SW620 and HT-29 cells were transfected with miR-486-5p mimic (miR-486-5p group), mimic control (NC group), and blank control (blank group). ( B ) Transfection of miR-486-5p mimics to SW620 and HT-29 cells increases the expression of miR-486-5p detected by qRT-PCR. ( C ) NRP2 protein expression level was significantly decreased in miR-486-5p-transfected SW620 and HT-29 cells. ( D ) Relative NRP2 protein levels in figure ( C ) were quantified using the gray scale quantification analysis. ** P <0.01. Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NRP2, neuropilin-2; qRT-PCR, quantitative real-time polymerase chain reaction; NC, nontarget control.

Article Snippet: Human CRC cell lines SW620 and HT-29 were purchased from Boster Company (Boster, Wuhan, People’s Republic of China).

Techniques: Expressing, Luciferase, Reporter Assay, Transfection, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: OncoTargets and therapy

Article Title: miR-486-5p attenuates tumor growth and lymphangiogenesis by targeting neuropilin-2 in colorectal carcinoma

doi: 10.2147/OTT.S103460

Figure Lengend Snippet: miR-486-5p inhibited xenograft tumor growth by targeting NRP2 in vivo. Notes: ( A ) Groups of nude mice were implanted subcutaneously with SW620/PBS cells (Blank group), SW620/mimic-control cells (NC group), or SW620/miR-486-5p cells (miR-486-5p group), Tumor volumes were recorded at the indicated times. ( B ) The tumor mass was determined when the mice were sacrificed. ( C ) NRP2 expression in tumors was examined by Western blot. ( D ) Quantitative analysis of the NRP2 protein level using a gray scan analysis. One representative tumor mass group from three independent experiments with similar results is shown. * P <0.05, ** P <0.01. Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NRP2, neuropilin-2; PBS, phosphate-buffered saline; NC, nontarget control.

Article Snippet: Human CRC cell lines SW620 and HT-29 were purchased from Boster Company (Boster, Wuhan, People’s Republic of China).

Techniques: In Vivo, Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: A Plant-Derived Antigen–Antibody Complex Induces Anti-Cancer Immune Responses by Forming a Large Quaternary Structure

doi: 10.3390/ijms21165603

Figure Lengend Snippet: Suppression of tumor growth in nude mice through administration of sera from mice immunized with GA P × CO P . BALB/c nu/nu mice were intradermally injected with 1 × 10 6 SW620 cells. Mice were subsequently injected with serum obtained from mice immunized with 1× PBS, GA M , GA P , GA M + CO M , or GA P × CO P (S (1× PBS), S (GA M ), S (GA P ), S (GA M + CO M ), or S (GA P × CO P ), respectively). The positive control was the CO M antibody treated group. At day 1, 4, and 7, all mice were injected with three additional doses of serum or CO M antibody. Tumor volumes (mm 3 ) were recorded at 8, 10, 12, and 15 days after the initial inoculation of cancer cells. The asterisks indicate statistically significant differences (** p < 0.01).

Article Snippet: The SW620 human colorectal carcinoma cells (1 × 10 6 ) were intradermally inoculated into the back of six-week-old BALB/c nu/nu mice (Japan SLC Inc., Hamamatsu, Shizuoka, Japan).

Techniques: Injection, Positive Control