Journal: Journal of cancer therapy

Article Title: Saporin Conjugated Monoclonal Antibody to the Transcobalamin Receptor TCblR/ CD 320 Is Effective in Targeting and Destroying Cancer Cells

doi: 10.4236/jct.2013.46122

Figure Lengend Snippet: TCblR expression profile and concentration of mAb-Saporin required for inhibiting cell proliferation.

Article Snippet: SW48 [SW-48] , ATCC CCL-231 , colorectal adenocarcinoma, epithelial , 35 , 20 , 37 , 18 , 10 , 7 , <0.05 , 1.7 , <0.05.

Techniques: Expressing, Concentration Assay, Binding Assay

Journal: Oncotarget

Article Title: Loss of p53-inducible long non-coding RNA LINC01021 increases chemosensitivity

doi: 10.18632/oncotarget.22245

Figure Lengend Snippet: (A) Expression of selected lncRNAs was determined by qPCR analyses after induction of ectopic p53 by addition of doxyxcycline (DOX) in SW480 cells for 24 hours and normalized to the expression in untreated cells and to GAPDH . (B-E) Expression of selected lncRNAs was determined by qPCR analyses after treatment of HCT116 p53 +/+ and p53 -/- cell lines with 5-FU, doxorubicin (DOXO), etoposide, Nutlin-3 or, as a control, DMSO for 24 hours, and normalized to the expression in untreated cells and to GAPDH . (F) LINC01021 expression was analyzed by qPCR in isogenic p53 +/+ and p53 -/- HCT116, RKO and SW48 cell lines and normalized to the expression in p53 +/+ cells and to GAPDH . (A-F) Results are represented as mean +/- s.d. ( n =3).

Article Snippet: The colorectal cancer cell lines HCT116, SW48 and RKO (all p53+/+ or p53-/-, respectively) were obtained from Bert Vogelstein (Johns Hopkins Medical School, Baltimore).

Techniques: Expressing, Control

Journal: Oncotarget

Article Title: Loss of p53-inducible long non-coding RNA LINC01021 increases chemosensitivity

doi: 10.18632/oncotarget.22245

Figure Lengend Snippet: (A) Western blot analysis of p53 and p21 expression after treatment of LINC01021 wt and KO HCT116 cell lines with DMSO or 5-FU for 12 hours. (B) Western blot analysis of p53 and p21 protein expression after treatment of LINC01021 wt and KO HCT116 cell lines with DMSO or doxorubicin (DOXO) for 12 (left) or 24 hours (right). (C) Western Blot analysis of p53 and p21 expression after treatment of SW48 p53+/+ expressing different LINC01021 isoforms with DMSO or 5-FU for the indicated time periods. Ectopic LINC01021 expression was induced by addition of doxycycline (DOX) for 32 hours (pre-treatment) before addition of 5-FU.

Article Snippet: The colorectal cancer cell lines HCT116, SW48 and RKO (all p53+/+ or p53-/-, respectively) were obtained from Bert Vogelstein (Johns Hopkins Medical School, Baltimore).

Techniques: Western Blot, Expressing

Journal: BMC Cancer

Article Title: Involvement of promoter methylation in the regulation of Pregnane X receptor in colon cancer cells

doi: 10.1186/1471-2407-11-81

Figure Lengend Snippet: Basal expression profile of colon cancer cell lines . Basal levels of (a), PXR exon 5-6; (b), PXR exon 1a-2; (c), CYP3A4; and (d), VDR transcripts in LS180, LoVo, Caco-2, HT29, HCT116 and SW48 cells. The vertical axis indicates a relative transcript level (ratio to LS180 cells). The transcripts indicated by * (PXR exon 5-6 transcript in SW48 cells and CYP3A4 transcript in HCT116 cells) were not detected and we estimated the minimum detectable levels among gradually diluted calibration samples (0.00257 and 0.0399, respectively).

Article Snippet: Human colorectal cancer cell lines LS180, Caco-2, HT29, HCT116, DLD-1, LoVo, SW48 and SW620 were purchased from DS Pharma Biomedical Co., Ltd. (Osaka, Japan).

Techniques: Expressing

Journal: BMC Cancer

Article Title: Involvement of promoter methylation in the regulation of Pregnane X receptor in colon cancer cells

doi: 10.1186/1471-2407-11-81

Figure Lengend Snippet: Expression profile of colon cancer cell lines after 5-aza-dC treatment . Levels of PXR, VDR and CYP3A4 transcripts in (a), LS180; (b), Caco-2; (c), HCT116; (d), LoVo; (e), HT29; and (f), SW48 cells. Cells were treated with 5-aza-2'-deoxycytidine (0, 0.5 or 5 μM). The vertical axis indicates a relative transcript level (ratio to cells without 5-aza-dC treatment). Numbers in italics indicate relative transcript levels that were over the maximum scale on the vertical axis. The transcripts indicated by * (PXR exon 5-6 transcript in SW48 cells and CYP3A4 transcript in HCT116 cells) were not detected and we estimated the minimum detectable levels among gradually diluted calibration samples.

Article Snippet: Human colorectal cancer cell lines LS180, Caco-2, HT29, HCT116, DLD-1, LoVo, SW48 and SW620 were purchased from DS Pharma Biomedical Co., Ltd. (Osaka, Japan).

Techniques: Expressing

Journal: BMC Cancer

Article Title: Involvement of promoter methylation in the regulation of Pregnane X receptor in colon cancer cells

doi: 10.1186/1471-2407-11-81

Figure Lengend Snippet: Profile of Microsatellite instability (MSI), mismatch repair (MMR) deficiency and promoter methylation in 6 colon cancer cell lines.

Article Snippet: Human colorectal cancer cell lines LS180, Caco-2, HT29, HCT116, DLD-1, LoVo, SW48 and SW620 were purchased from DS Pharma Biomedical Co., Ltd. (Osaka, Japan).

Techniques: Methylation

Journal: Cell Death and Differentiation

Article Title: DUB3 deubiquitinates and stabilizes NRF2 in chemotherapy resistance of colorectal cancer

doi: 10.1038/s41418-019-0303-z

Figure Lengend Snippet: DUB3 is a potential DUB of NRF2 and correlated expressed with NRF2 in colon cancer. a DUB3 has the strongest effect on NRF2 protein level among the top 10 DUBs. HEK293T cells were cotransfected with NRF2-Myc and individual top 10 DUBs-Flag overexpresiong vectors. Forty-eight hours after transfection, the cell lysates were analyzed by western blot with the indicated antibodies. NRF2-Myc protein expression was normalized to GAPDH. b DUB3 has the strongest effect on NRF2 transcriptional activity among the top 10 DUBs. HEK293T cells were cotransfected with ARE reporter firefly luciferase (100 ng), pRL-TK (10 ng) and the indicated amounts of DUBs. Reporter assays were performed 24 h after transfection, and the results are presented as the NRF2/TK luciferase activity. c Oncomine data mining analysis of NRF2 mRNA levels in three different datasets comparing normal tissues vs. CRC tissues. d The protein expression of NRF2 and DUB3 in 24 representative pairs of primary CRC (T) and adjacent non-tumor tissues (N). e The protein expression of NRF2 and DUB3 in the human embryonic kidney cell line HEK293T, the normal human colon cell line FHC and seven colorectal cell lines (HCT116, SW48, SW480, RKO, DLD1, LOVO, and HT29)

Article Snippet: Cell culture Human CRC cell lines including HCT116, SW48, SW480, RKO, DLD1, LOVO, HT29, the human normal colon cell line FHC, the human embryonic kidney 293T cell line (HEK293T), and the human osteosarcoma cell line U2OS were purchased from China Center for Type Culture Collection (CCTCC).

Techniques: Transfection, Western Blot, Expressing, Activity Assay, Luciferase