Journal: Cell Reports Medicine

Article Title: Vaccine-induced ICOS + CD38 + circulating Tfh are sensitive biosensors of age-related changes in inflammatory pathways

doi: 10.1016/j.xcrm.2021.100262

Figure Lengend Snippet:

Article Snippet: Anti-human Survivin (REA459) , Miltenyi , Cat # 130-106-741; RRID: AB_2653606.

Techniques: Recombinant, Staining, Gene Expression, Software

Journal: BioMed Research International

Article Title: Improvement of Inflammation through Antioxidant Pathway of Gardeniae Fructus 50% EtOH Extract (GE) from Acute Reflux Esophagitis Rats

doi: 10.1155/2020/4826176

Figure Lengend Snippet: Western blot analysis of (a) survivin, (b) cytochrome c, (c) caspase-3, (d) Bax, and (e) Bcl-2 expressions. Nor: normal rats, Con: reflux esophagitis control rats, GL: GE 50 mg/kg treated reflux esophagitis rats, and GH: GE 100 mg/kg treated reflux esophagitis rats. (f) All data are expressed means ± SEM, ( n = 6) rats per group. Significance: ∗ p < 0.05, ∗∗ p < 0.01 versus RE control rat values.

Article Snippet: Mouse monoclonal antibodies against survivin (1 : 1,000, NB 500-205) were purchased from Novus Biologicals (Littleton, CO, USA).

Techniques: Western Blot, Reflux, Control

Journal: Scientific reports

Article Title: The proto-oncogene survivin splice variant 2B is induced by PDGF and leads to cell proliferation in rheumatoid arthritis fibroblast-like synoviocytes.

doi: 10.1038/srep09795

Figure Lengend Snippet: Figure 1. The survivin expression in blood and synovial tissues from RA and OA patients. (a) The serum survivin expression levels were analyzed using an ELISA. (RA, 22 cases; controls, 16 cases) (mean RA, 19.5 pg/ml vs. controls, 7.7 pg/ml; p = 0.044). An age- and sex-matched control group consisted of OA and healthy controls (RA, 58.8 ± 15.6 years old; controls, 53.9 ± 19.8 years old; p = 0.404). t-test, *p < 0.05. (b) The survivin expressions in RA and OA synovial tissues were analyzed using IHC (survivin: red, upper sections x40, lower sections x20). The data shown are representative of the samples. Arrows indicate the endothelium of synovia. (c–f) Dual staining in immunohistochemistry for RA synovial tissues. Red pigment and arrows indicate the expression of each lineage marker (CD55; a marker of FLS, CD4; a marker of CD4+ T lymphocytes, CD8; a marker of CD8+ T lymphocytes, MPO (myeloperoxidase); a marker of neutrophil). Green pigment indicates survivin. (x40)

Article Snippet: The serum survivin levels were measured with a Quantikine ELISA kit for human survivin (R&D systems) according to the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control, Staining, Immunohistochemistry, Marker

Journal: Scientific reports

Article Title: The proto-oncogene survivin splice variant 2B is induced by PDGF and leads to cell proliferation in rheumatoid arthritis fibroblast-like synoviocytes.

doi: 10.1038/srep09795

Figure Lengend Snippet: Figure 6. The serum expression levels of survivin-2B in RA patients determined using an ELISA. (a) We collected serum samples from 35 RA patients and 21 age- and sex- matched controls (healthy controls and OA patients), and measured the serum survivin-2B levels with an ELISA (mean RA, 18.7 ng/ml vs. controls, 4.7 ng/ml; p = 0.049) (RA, 64.7 ± 11.4 years old; controls, 59.6 ± 18.0 years old; p = 0.250). The gray lines indicate the median of survivin-2B level in each group. (b) We compared the DAS28-ESR between the survivin-2B high RA group (n = 18) and the survivin-2B low RA group (n = 21) (mean RA, 4.64 vs. controls, 3.64; p = 0.037). t-test, ∗p < 0.05.

Article Snippet: The serum survivin levels were measured with a Quantikine ELISA kit for human survivin (R&D systems) according to the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: British Journal of Cancer

Article Title: Expression of cytoplasmic and nuclear Survivin in primary and secondary human glioblastoma

doi: 10.1038/sj.bjc.6602904

Figure Lengend Snippet: Immunohistochemical staining of Survivin and fluorescent TUNEL staining in human gliomas ( A–G ). A primary glioblastoma showed a high level (++++) expression of nuclear Survivin ( A ). High staining score (++++) of cytoplasmic Survivin was detected in another primary glioblastoma ( B ). Moderate expression level (++) of cytoplasmic Survivin was detected in a pre-existing low-graded (grade II) glioma ( C ), which was obviously lower than that in its paired secondary glioblastoma with a staining score of ++++ ( D ). High-level (++++) expression of nuclear Survivin was detected in a secondary glioblastoma ( E ) and its matched pre-existing grade II glioma ( F ). Three apoptotic cells with clear nuclear staining (green color) were examined in a primary glioblastoma ( G ).

Article Snippet: The tumour slides were incubated at 4°C overnight in a moist chamber with one of the two polyclonal anti-Survivin antibodies (NB-500-201 K3, Novus Biologicals, Littleton, CO, USA, 1 : 300 dilution and SC-10811, Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 400 dilution).

Techniques: Immunohistochemical staining, Staining, TUNEL Assay, Expressing

Journal: British Journal of Cancer

Article Title: Expression of cytoplasmic and nuclear Survivin in primary and secondary human glioblastoma

doi: 10.1038/sj.bjc.6602904

Figure Lengend Snippet: Status of Survivin expression and apoptotic index in primary GBMs

Article Snippet: The tumour slides were incubated at 4°C overnight in a moist chamber with one of the two polyclonal anti-Survivin antibodies (NB-500-201 K3, Novus Biologicals, Littleton, CO, USA, 1 : 300 dilution and SC-10811, Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 400 dilution).

Techniques: Expressing

Journal: British Journal of Cancer

Article Title: Expression of cytoplasmic and nuclear Survivin in primary and secondary human glioblastoma

doi: 10.1038/sj.bjc.6602904

Figure Lengend Snippet: Status of Survivin expression and apoptotic index in secondary GBMs

Article Snippet: The tumour slides were incubated at 4°C overnight in a moist chamber with one of the two polyclonal anti-Survivin antibodies (NB-500-201 K3, Novus Biologicals, Littleton, CO, USA, 1 : 300 dilution and SC-10811, Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 400 dilution).

Techniques: Expressing

Journal: British Journal of Cancer

Article Title: Expression of cytoplasmic and nuclear Survivin in primary and secondary human glioblastoma

doi: 10.1038/sj.bjc.6602904

Figure Lengend Snippet: The expression of cytoplasmic and nuclear Survivin in 15 paired secondary GBMs and pre-existing lesions a

Article Snippet: The tumour slides were incubated at 4°C overnight in a moist chamber with one of the two polyclonal anti-Survivin antibodies (NB-500-201 K3, Novus Biologicals, Littleton, CO, USA, 1 : 300 dilution and SC-10811, Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 400 dilution).

Techniques: Expressing