supplements Search Results


md vs  (ATCC)
97
ATCC md vs
Md Vs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pmc10299816-748-5-3?v=ATCC
Average 97 stars, based on 1 article reviews
md vs - by Bioz Stars, 2026-08
97/100 stars
  Buy from Supplier
panel track supplement  (Dexcom Inc)
86
Dexcom Inc panel track supplement
Panel Track Supplement, supplied by Dexcom Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/fda_document____cdrh_docs_slash_pdf12_slash_p120005s041c-2824-50-63?v=Dexcom+Inc
Average 86 stars, based on 1 article reviews
panel track supplement - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier
stemxvivo chondrogenic supplement  (R&D Systems)
94
R&D Systems stemxvivo chondrogenic supplement
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
Stemxvivo Chondrogenic Supplement, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pmc09076650-347-18-22?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
stemxvivo chondrogenic supplement - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
n21  (R&D Systems)
95
R&D Systems n21
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
N21, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pmc07889698-128-44-46?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
n21 - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
n2 max  (R&D Systems)
95
R&D Systems n2 max
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
N2 Max, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pmc11848315__pnas__2422631122__sapp-46-19-23?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
n2 max - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
media supplement  (R&D Systems)
94
R&D Systems media supplement
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
Media Supplement, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pmc11219314-118-2-4?v=R%26D+Systems
Average 94 stars, based on 1 article reviews
media supplement - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
n21 max media supplement 50x  (R&D Systems)
95
R&D Systems n21 max media supplement 50x
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
N21 Max Media Supplement 50x, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pm39629121-300-8-12?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
n21 max media supplement 50x - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
media supplement  (Beyotime)
99
Beyotime media supplement
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
Media Supplement, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pm39836491-403-34-37?v=Beyotime
Average 99 stars, based on 1 article reviews
media supplement - by Bioz Stars, 2026-08
99/100 stars
  Buy from Supplier
genetic systems hiv  (Bio-Rad)
95
Bio-Rad genetic systems hiv
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
Genetic Systems Hiv, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pmc10902925-105-6-12?v=Bio-Rad
Average 95 stars, based on 1 article reviews
genetic systems hiv - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier
vitamin mix atcc atcc md vs voriconazole cayman  (ATCC)
94
ATCC vitamin mix atcc atcc md vs voriconazole cayman
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
Vitamin Mix Atcc Atcc Md Vs Voriconazole Cayman, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pm32526207-271-33-35?v=ATCC
Average 94 stars, based on 1 article reviews
vitamin mix atcc atcc md vs voriconazole cayman - by Bioz Stars, 2026-08
94/100 stars
  Buy from Supplier
t 75 flasks  (Cell Applications Inc)
96
Cell Applications Inc t 75 flasks
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
T 75 Flasks, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/bio_rxiv__64898__2026__04__03__716316-186-4-10?v=Cell+Applications+Inc
Average 96 stars, based on 1 article reviews
t 75 flasks - by Bioz Stars, 2026-08
96/100 stars
  Buy from Supplier
n2 supplement  (R&D Systems)
95
R&D Systems n2 supplement
A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and <t>chondrogenic</t> ( d , h ) media. Scale bars, 100 µm.
N2 Supplement, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/supplements/pmc06135742-184-10-13?v=R%26D+Systems
Average 95 stars, based on 1 article reviews
n2 supplement - by Bioz Stars, 2026-08
95/100 stars
  Buy from Supplier

Image Search Results


A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and chondrogenic ( d , h ) media. Scale bars, 100 µm.

Journal: Nature Communications

Article Title: A FoxA2+ long-term stem cell population is necessary for growth plate cartilage regeneration after injury

doi: 10.1038/s41467-022-30247-1

Figure Lengend Snippet: A Tibia ( a ) and ulna ( b ) from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen (Tam) P13-P17 and harvested at P18, and from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;PTHrP mcherry mice treated with tamoxifen P13-P17 and harvested at P40 ( c ). Florescence microscopy for ZsGreen (green), mcherry (red), Hoechst (blue). Scale bars, 50 µm. Separation of FoxA2+(green) cells from PTHrP+(red) cells and double positive FoxA2+PTHrP+(yellow) cells by Fluorescence-activated cell sorting (FACS) of GP cells isolated from FoxA2 CreERT2/+ ;ZsGreen fl/+ ; PTHrP mcherry mice, injected with tamoxifen from P13 to P17, and harvested at P18 ( d ) or P40 ( e ). B Colony forming unit (cfu) assay for FoxA2+(ZsGreen+) cells (from FoxA2 CreERT2/+ ;ZsGreen fl/+ ;col.10 mcherry mice treated with tamoxifen P13-P17 and harvested at P18) and PTHrP+(mcherry+)cells (from PTHrP mcherry mice harvested at P18). Quantification of the number of colonies (per 10 3 cells) presented as mean ± SD from n = 3 experiments. C Trilineage differentiation of FoxA2+(ZsGreen+) clones, from passage P1 ( a – d ) and passage P9 ( e – h ). Four independent clones, for each passage, were tested in either growth media ( a , e ), or adipogenic ( b , f ), osteogenic ( c , g ) and chondrogenic ( d , h ) media. Scale bars, 100 µm.

Article Snippet: Chondrogenesis was induced by culturing cell pellets in StemXVivo chondrogenic base media (CCM005, R&D Systems, USA) supplemented with StemXVivo chondrogenic supplement (CCM006, R&D Systems, USA) according to procedures described by manufacturer’s instructions.

Techniques: Microscopy, Fluorescence, FACS, Isolation, Injection, Colony-forming Unit Assay, Clone Assay

FoxA2+ LTSSC, residual from epiphyseal cartilage postnatal development, located on the outskirts of the SOC, contribute to the top compartment of the RZ. FoxA2+ cells are distinct from the short-term PTHrP+ stem cells located at the bottom of the RZ. Prior to P28, FoxA2+ cells have a dual osteo-chondro-progenitor fate, contributing to the chondrogenic lineage (by forming columnar stacks of chondrocytes in the GP), and the osteogenic lineage (by forming col.1+ bone cells in the SOC). After P28, FoxA2+ LTSSC remain mostly in GP, and their contribution to the SOC is reduced. GP injury activates FoxA2+ cells to undergo proliferative expansion and to provide a framework for the regenerating tissue. In conclusion, FoxA2+ cells, located in the top compartment of the RZ, are a PTHrP-(negative) long-term skeletal stem cell (LT-SSC) population necessary for both GP turnover and cartilage regeneration following injury. SOC = secondary ossification center, RZ = resting zone, PZ = proliferating zone, HZ = hypertrophic zone.

Journal: Nature Communications

Article Title: A FoxA2+ long-term stem cell population is necessary for growth plate cartilage regeneration after injury

doi: 10.1038/s41467-022-30247-1

Figure Lengend Snippet: FoxA2+ LTSSC, residual from epiphyseal cartilage postnatal development, located on the outskirts of the SOC, contribute to the top compartment of the RZ. FoxA2+ cells are distinct from the short-term PTHrP+ stem cells located at the bottom of the RZ. Prior to P28, FoxA2+ cells have a dual osteo-chondro-progenitor fate, contributing to the chondrogenic lineage (by forming columnar stacks of chondrocytes in the GP), and the osteogenic lineage (by forming col.1+ bone cells in the SOC). After P28, FoxA2+ LTSSC remain mostly in GP, and their contribution to the SOC is reduced. GP injury activates FoxA2+ cells to undergo proliferative expansion and to provide a framework for the regenerating tissue. In conclusion, FoxA2+ cells, located in the top compartment of the RZ, are a PTHrP-(negative) long-term skeletal stem cell (LT-SSC) population necessary for both GP turnover and cartilage regeneration following injury. SOC = secondary ossification center, RZ = resting zone, PZ = proliferating zone, HZ = hypertrophic zone.

Article Snippet: Chondrogenesis was induced by culturing cell pellets in StemXVivo chondrogenic base media (CCM005, R&D Systems, USA) supplemented with StemXVivo chondrogenic supplement (CCM006, R&D Systems, USA) according to procedures described by manufacturer’s instructions.

Techniques: