superscript™ iii reverse transcript kit Search Results


rt pcr kit  (PCR Biosystems Ltd)
94
PCR Biosystems Ltd rt pcr kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Rt Pcr Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript%E2%84%A2+iii+reverse+transcript+kit/1-Step+Go+RT-PCR+Kit/pmc12818604-276-12-14
Average 94 stars, based on 1 article reviews
rt pcr kit - by Bioz Stars, 2026-09
94/100 stars
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starscript iii reverse transcription kit  (GenStar Biosolutions)
90
GenStar Biosolutions starscript iii reverse transcription kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Starscript Iii Reverse Transcription Kit, supplied by GenStar Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript%E2%84%A2+iii+reverse+transcript+kit/starscript+iii+reverse+transcription+kit/pmc09641444-97-20-24
Average 90 stars, based on 1 article reviews
starscript iii reverse transcription kit - by Bioz Stars, 2026-09
90/100 stars
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superscripttm iii reverse transcription kit  (Promega)
90
Promega superscripttm iii reverse transcription kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Superscripttm Iii Reverse Transcription Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript%E2%84%A2+iii+reverse+transcript+kit/superscripttm+iii+reverse+transcription+kit/pm33245959-108-11-16
Average 90 stars, based on 1 article reviews
superscripttm iii reverse transcription kit - by Bioz Stars, 2026-09
90/100 stars
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hiscript iii rt supermix for qpcr +gdna wiper  (Vazyme Biotech Co)
99
Vazyme Biotech Co hiscript iii rt supermix for qpcr +gdna wiper
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Hiscript Iii Rt Supermix For Qpcr +Gdna Wiper, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript%E2%84%A2+iii+reverse+transcript+kit/HiScript+III+RT+SuperMix+for+qPCR+%2BgDNA+wiper/custom%40r323%4010%2E1016%2Fj%2Etheriogenology%2E2024%2E11%2E003
Average 99 stars, based on 1 article reviews
hiscript iii rt supermix for qpcr +gdna wiper - by Bioz Stars, 2026-09
99/100 stars
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nanjing novozymes reverse transcription kit hiscript iii rt supermix for qpcr (+gdna wiper)  (Novozymes limited)
90
Novozymes limited nanjing novozymes reverse transcription kit hiscript iii rt supermix for qpcr (+gdna wiper)
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Nanjing Novozymes Reverse Transcription Kit Hiscript Iii Rt Supermix For Qpcr (+Gdna Wiper), supplied by Novozymes limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript%E2%84%A2+iii+reverse+transcript+kit/nanjing+novozymes+reverse+transcription+kit+hiscript+iii+rt+supermix+for+qpcr+++gdna+wiper+/10__1134_slash_s1021443722601483-50-43-44
Average 90 stars, based on 1 article reviews
nanjing novozymes reverse transcription kit hiscript iii rt supermix for qpcr (+gdna wiper) - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
superscript iii first-strand cdna synthesis improm-ii reverse transcriptase kit  (Promega)
90
Promega superscript iii first-strand cdna synthesis improm-ii reverse transcriptase kit
(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 <t>mutant.</t> <t>RT-PCR</t> was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.
Superscript Iii First Strand Cdna Synthesis Improm Ii Reverse Transcriptase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript%E2%84%A2+iii+reverse+transcript+kit/superscript+iii+first+strand+cdna+synthesis+improm+ii+reverse+transcriptase+kit/10__1111_slash_ppa__13029-80-10-24
Average 90 stars, based on 1 article reviews
superscript iii first-strand cdna synthesis improm-ii reverse transcriptase kit - by Bioz Stars, 2026-09
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Image Search Results


(A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

Journal: PLOS Pathogens

Article Title: Peptidoglycan architecture dictates protein interactions, tissue tropism, and arthritis in the Lyme disease spirochete Borrelia burgdorferi

doi: 10.1371/journal.ppat.1013849

Figure Lengend Snippet: (A) Schematic of the putative bb0605 operon, as well as the downstream bb0608 locus, mapped from the B. burgdorferi B31 type strain genome. The transposon integration site (+300, 5’ to 3’) in bb0605 is shown (triangle) as are the length, and location, of predicted non-coding regions. (B) Relative mRNA levels of target loci to validate B31-5A3/ bb0605 mutant. RT-PCR was performed on RNA isolated from both parental (B31-5A3, charcoal) and mutant (B31-5A3/ bb0605, light gray) strains using locus-specific primers and normalized relative to constitutively expressed flaB. Values are the mean + /- SD. n.d., not detected after 45 cycles. (C) Identifying changes in the peptidoglycan composition of B31-5A3/ bb0605 . Liquid chromatogram of mutanolysin treated PG isolated from B31-5A3 (top, in black) and B31-5A3/ bb0605 (bottom, in green). Each numbered peak corresponds to a muropeptide found in and , respectively. Peak 5 (shaded gray) corresponds to the muropeptide Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala). (D) MS (top) and MS/MS (bottom) of the Glc N Ac-Mur N Ac-Ala-Glu-Orn-[Gly]-Ala-Ala muropeptide. MS/MS fragmentation (inset) data confirms the pentapeptide(-Gly) structure.

Article Snippet: All reactions were performed on the same plate, using PCRBIO 1-Step Go RT-PCR Kit (PCR Biosystems) following the recommended procedures.

Techniques: Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Isolation, Tandem Mass Spectroscopy