Journal: Development (Cambridge, England)

Article Title: Daw1 regulates the timely onset of cilia motility during development

doi: 10.1242/dev.200017

Figure Lengend Snippet: Body axis curvature self-corrects after delayed onset cilia motility. (A-E) The first 5 days of development show daw1 b1403 mutants self-correct curves. (A′-E′) SuperPlots of body angle, θ (inset in A′). Pairwise comparisons used two-way ANOVA adjusted for multiple comparisons. (F-I) TICS of CC cilia motility at different time points. (F′-I′) SuperPlots of the proportion of the CC covered by cilia motility (motile area). *** P <0.001, ** P <0.01, * P <0.05 (two-tailed unpaired t -tests). ns, not significant. Scale bars: 0.5 mm (A-E); 5 µm (F-I).

Article Snippet: All plots, including SuperPlots , were generated using GraphPad version 8.4.3, except UMAP plots, which were generated in R. For plots showing body angles, dots represent individual embryos; triangles represent clutch averages and black lines show population means.

Techniques: Two Tailed Test

Journal: Development (Cambridge, England)

Article Title: Daw1 regulates the timely onset of cilia motility during development

doi: 10.1242/dev.200017

Figure Lengend Snippet: daw1 b1403 encodes a loss-of-function protein. (A-C) Lateral views of controls and daw1 and cfap298 crispants. (A′-C′) SuperPlots of body angle quantitation. Pairwise comparisons used two-way ANOVA adjusted for multiple comparisons. *** P <0.001, ** P <0.01, * P <0.05. ns, not significant. (D) Expression of Daw1 WT but not Daw1 b1403 rescues the body curves of daw1 crispants. Scale bars: 0.5 mm (A-D).

Article Snippet: All plots, including SuperPlots , were generated using GraphPad version 8.4.3, except UMAP plots, which were generated in R. For plots showing body angles, dots represent individual embryos; triangles represent clutch averages and black lines show population means.

Techniques: Quantitation Assay, Expressing

Journal: Development (Cambridge, England)

Article Title: Daw1 regulates the timely onset of cilia motility during development

doi: 10.1242/dev.200017

Figure Lengend Snippet: Self-correction of curves can occur throughout developmental stages. (A-C) Top: TICS of CC cilia motility of 25 hpf cfap298 tm304 mutants raised at 30°C (A), then 52 hpf after maintaining 30°C (B) or downshifting to 22°C at 24 hpf (C). Bottom: SuperPlots of motility data. Insets: axis straightening occurred in downshifted embryos but not those maintained at 30°C. (D) Schematic of temperature (T) shifts. Embryos were raised at 30°C until either 1, 2, 3, 4 or 5 dpf, then downshifted to 22°C, inducing cilia motility. Body angles were assessed until 7 dpf. (E) Body angles across 1-7 dpf after temperature downshifts on days 1-5 (D1-D5). (F) Summary showing zebrafish larvae self-correct curves after robust CC cilia motility onsets. Distance around the sundial represents later times of motility onset in distinct conditions. **** P <0.0001, * P <0.05 (two-tailed unpaired t -tests). Scale bars: 5 µm (A-C).

Article Snippet: All plots, including SuperPlots , were generated using GraphPad version 8.4.3, except UMAP plots, which were generated in R. For plots showing body angles, dots represent individual embryos; triangles represent clutch averages and black lines show population means.

Techniques: Two Tailed Test

Journal: BMC Genomics

Article Title: Transcriptomic Profile of Lin − Sca1 + c-kit (LSK) cells in db/db mice with long-standing diabetes

doi: 10.1186/s12864-024-10679-3

Figure Lengend Snippet: Validation of novel miRNAs and mRNA using qPCR in db/db mice. The top 3 downregulated miRNAs (miR-3968, miR-1971 & and miR-148a-3p), miR-126a-5p, and their mRNA targets were analyzed using real-time quantitative PCR (qRT-PCR). (a) Gene expression analysis of miR-3968, miR-1971, miR-148a-3p, and miR-126a-5p normalized to U6 (b) Gene expression analysis of mRNA targets normalized to TBP. The figure is presented as a SuperPlot generated using GraphPad Prism, where solid dots represent biological replicates (mean ± SEM; N = 5) and small square boxes represent technical replicates. The data are analyzed on technical replicates using student unpaired t-test ( N = 5*3 = 15)

Article Snippet: The figure is presented as a SuperPlot generated using GraphPad Prism, where solid dots represent biological replicates (mean ± SEM; N = 5) and small square boxes represent technical replicates.

Techniques: Biomarker Discovery, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression, Generated