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superplot generated using graphpad prism  (GraphPad Software Inc)
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GraphPad Software Inc superplot generated using graphpad prism
Validation of novel miRNAs and mRNA using qPCR in db/db mice. The top 3 downregulated miRNAs (miR-3968, miR-1971 & and miR-148a-3p), miR-126a-5p, and their mRNA targets were analyzed using real-time quantitative PCR (qRT-PCR). (a) Gene expression analysis of miR-3968, miR-1971, miR-148a-3p, and miR-126a-5p normalized to U6 (b) Gene expression analysis of mRNA targets normalized to TBP. The figure is presented as a <t>SuperPlot</t> generated using GraphPad Prism, where solid dots represent biological replicates (mean ± SEM; N = 5) and small square boxes represent technical replicates. The data are analyzed on technical replicates using student unpaired t-test ( N = 5*3 = 15)
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Validation of novel miRNAs and mRNA using qPCR in db/db mice. The top 3 downregulated miRNAs (miR-3968, miR-1971 & and miR-148a-3p), miR-126a-5p, and their mRNA targets were analyzed using real-time quantitative PCR (qRT-PCR). (a) Gene expression analysis of miR-3968, miR-1971, miR-148a-3p, and miR-126a-5p normalized to U6 (b) Gene expression analysis of mRNA targets normalized to TBP. The figure is presented as a SuperPlot generated using GraphPad Prism, where solid dots represent biological replicates (mean ± SEM; N = 5) and small square boxes represent technical replicates. The data are analyzed on technical replicates using student unpaired t-test ( N = 5*3 = 15)

Journal: BMC Genomics

Article Title: Transcriptomic Profile of Lin − Sca1 + c-kit (LSK) cells in db/db mice with long-standing diabetes

doi: 10.1186/s12864-024-10679-3

Figure Lengend Snippet: Validation of novel miRNAs and mRNA using qPCR in db/db mice. The top 3 downregulated miRNAs (miR-3968, miR-1971 & and miR-148a-3p), miR-126a-5p, and their mRNA targets were analyzed using real-time quantitative PCR (qRT-PCR). (a) Gene expression analysis of miR-3968, miR-1971, miR-148a-3p, and miR-126a-5p normalized to U6 (b) Gene expression analysis of mRNA targets normalized to TBP. The figure is presented as a SuperPlot generated using GraphPad Prism, where solid dots represent biological replicates (mean ± SEM; N = 5) and small square boxes represent technical replicates. The data are analyzed on technical replicates using student unpaired t-test ( N = 5*3 = 15)

Article Snippet: The figure is presented as a SuperPlot generated using GraphPad Prism, where solid dots represent biological replicates (mean ± SEM; N = 5) and small square boxes represent technical replicates.

Techniques: Biomarker Discovery, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression, Generated