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Image Search Results
Journal: bioRxiv
Article Title: Spatial localization of CD16a at the human NK cell ADCC lytic synapse
doi: 10.1101/2024.08.09.605851
Figure Lengend Snippet: (A-C) The far-left column depicts a cartoon representation of activating conditions used to model CD16a mediated activation and displays representative images of NK-92 CD16a-SNAP confocal and MINFLUX data of immune synapses on coated glass. From left to right, images show confocal reference, MINFLUX data colored given through DBSCAN, a zoomed in region of MINFLUX localizations as marked in the overview, and trace centers (single localizations are colored in purple, and isolated pairs are in orange) overlayed on raw MINFLUX data (colored in light cyan). Conditions are glass coated with (A) α-CD16 and α-CD18 antibodies, (B) α-CD18 antibody, and (C) PLL. (D) Average Ripley’s H function (top) and individual region of interest (ROI) Ripley’s H function (bottom) of MINFLUX data. (E) Nearest neighbor analysis of all MINFLUX data. (F) Nearest neighbor analysis of isolated pairs of CD16a molecules. (G) The total number of isolated CD16a pairs observed in all ROIs. Scale bars in confocal images and raw MINFLUX renderings represent 500 nm and zoomed in region scale bars represent 50 nm.
Article Snippet: JM is an employee of the company
Techniques: Activation Assay, Isolation
Journal: bioRxiv
Article Title: Spatial localization of CD16a at the human NK cell ADCC lytic synapse
doi: 10.1101/2024.08.09.605851
Figure Lengend Snippet: (A-B) The left column depicts a cartoon representation of activating conditions used to model CD16a mediated activation. Representative images of NK-92 CD16-SNAP confocal and MINFLUX data of immune synapse on SLBs. From left to right, images show confocal reference, MINFLUX data colored by ID given through DBSCAN, a zoomed in region of MINFLUX localizations as marked in the overview, and trace centers (single localizations are colored in purple, and isolated pairs are in orange) overlayed on raw MINFLUX data (colored in light cyan). SLBs contained HER2 and ICAM-1 (A) without Trastuzumab (-Traz) and (B) with Trastuzumab (+ Traz). (C) Average Ripley’s H function (top) and individual ROI Ripley’s H function (bottom) of MINFLUX data. (D) Nearest neighbor analysis of all MINFLUX data. (E) Nearest neighbor analysis of isolated pairs of CD16a molecules. Scale bars in confocal images and raw MINFLUX data represent 500 nm and zoomed in region scale bars represent 50 nm. MINFLUX microscopy data were collected across at least three independent experiments with a total of 12 cells analyzed.
Article Snippet: JM is an employee of the company
Techniques: Activation Assay, Isolation, Microscopy
Journal: bioRxiv
Article Title: Spatial localization of CD16a at the human NK cell ADCC lytic synapse
doi: 10.1101/2024.08.09.605851
Figure Lengend Snippet: MINFLUX data of CD16a localization demonstrated that CD16a often is found in pairs with an intra-fluorophore distance of ∼17 nm apart. Shown is a cartoon representation of what this could look like in un-scaffolded CD16a (left) versus CD16a scaffolded by a homodimer such as pCD3ζ.
Article Snippet: JM is an employee of the company
Techniques: