sup t1 cells Search Results


mouse macrophage cell line  (ATCC)
96
ATCC mouse macrophage cell line
Mouse Macrophage Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse macrophage cell line/product/ATCC
Average 96 stars, based on 1 article reviews
mouse macrophage cell line - by Bioz Stars, 2025-04
96/100 stars
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hhc6548 t1 m1 cells  (CLS Cell Lines Service GmbH)
90
CLS Cell Lines Service GmbH hhc6548 t1 m1 cells
Hhc6548 T1 M1 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hhc6548 t1 m1 cells/product/CLS Cell Lines Service GmbH
Average 90 stars, based on 1 article reviews
hhc6548 t1 m1 cells - by Bioz Stars, 2025-04
90/100 stars
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cyct1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc cyct1
Depletion of BRD4 via siBRD4 and JQ1 does not affect the transcriptional activity of AIRE. A, AIRE activates the human insulin promoter despite the depletion and inhibition of BRD4. Relative luciferase activities from hIns.Luc in the presence of mh.AIRE alone (bars 1–4) and with depleted BRD4 (bars 5–8), absent JQ1 (bars 1, 2, 5, and 6), and increasing amounts of JQ1 (bars 3, 4, 7, and 8) are presented as -fold activation above levels of the empty vector alone (bar 1). B, levels of informative proteins are presented in WBs. Levels of mh.AIRE (top panel), released BRD4 (second panel), total BRD4 (bottom panel, below the black line), and GAPDH (third panel) are presented. C, AIRE activates the KRT14 gene (AIRE-responsive) independently of BRD4. Relative RNA levels of KRT14 in the presence of mh.AIRE alone (bars 1–4) or with siBRD4 (bars 5–8) and increasing amounts of JQ1 (bars 3, 4, 7, and 8) or in the absence of JQ1 (bars 1, 2, 5, and 6) are presented as -fold activation above levels of the empty vector alone (bar 1). D, the CCNH gene (AIRE-nonresponsive) is not affected by AIRE or BRD4. Relative RNA levels are presented by the same analyses and conditions as in C. E–G, AIRE interacts with P-TEFb directly under physiological conditions. For AIRE immunoprecipitations (E), interactions between the ectopically expressed AIRE (bottom panel) and endogenous BRD4, <t>CycT1,</t> and CDK9 proteins (top to third panels) are presented in the presence or absence of siBRD4, compared with the IgG control (lane 1). CycT1 immunoprecipitations (F) were performed under the same conditions. Interactions between endogenous CycT1 (bottom panel) and BRD4, AIRE, and CDK9 (top to third panels) proteins are presented. G, WBs reveal levels of input proteins for E and F. Levels of total BRD4 (450 mm NaCl extraction) are presented in the bottom panel below the black line. For A, C, and D, error bars represent S.E., n = 3. Statistical significance is indicated by asterisks (paired t test; *, p < 0.05; **, p < 0.01).
Cyct1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cyct1/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
cyct1 - by Bioz Stars, 2025-04
95/100 stars
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magnetic cell total rna kit  (tiangen biotech co)
93
tiangen biotech co magnetic cell total rna kit
Depletion of BRD4 via siBRD4 and JQ1 does not affect the transcriptional activity of AIRE. A, AIRE activates the human insulin promoter despite the depletion and inhibition of BRD4. Relative luciferase activities from hIns.Luc in the presence of mh.AIRE alone (bars 1–4) and with depleted BRD4 (bars 5–8), absent JQ1 (bars 1, 2, 5, and 6), and increasing amounts of JQ1 (bars 3, 4, 7, and 8) are presented as -fold activation above levels of the empty vector alone (bar 1). B, levels of informative proteins are presented in WBs. Levels of mh.AIRE (top panel), released BRD4 (second panel), total BRD4 (bottom panel, below the black line), and GAPDH (third panel) are presented. C, AIRE activates the KRT14 gene (AIRE-responsive) independently of BRD4. Relative RNA levels of KRT14 in the presence of mh.AIRE alone (bars 1–4) or with siBRD4 (bars 5–8) and increasing amounts of JQ1 (bars 3, 4, 7, and 8) or in the absence of JQ1 (bars 1, 2, 5, and 6) are presented as -fold activation above levels of the empty vector alone (bar 1). D, the CCNH gene (AIRE-nonresponsive) is not affected by AIRE or BRD4. Relative RNA levels are presented by the same analyses and conditions as in C. E–G, AIRE interacts with P-TEFb directly under physiological conditions. For AIRE immunoprecipitations (E), interactions between the ectopically expressed AIRE (bottom panel) and endogenous BRD4, <t>CycT1,</t> and CDK9 proteins (top to third panels) are presented in the presence or absence of siBRD4, compared with the IgG control (lane 1). CycT1 immunoprecipitations (F) were performed under the same conditions. Interactions between endogenous CycT1 (bottom panel) and BRD4, AIRE, and CDK9 (top to third panels) proteins are presented. G, WBs reveal levels of input proteins for E and F. Levels of total BRD4 (450 mm NaCl extraction) are presented in the bottom panel below the black line. For A, C, and D, error bars represent S.E., n = 3. Statistical significance is indicated by asterisks (paired t test; *, p < 0.05; **, p < 0.01).
Magnetic Cell Total Rna Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/magnetic cell total rna kit/product/tiangen biotech co
Average 93 stars, based on 1 article reviews
magnetic cell total rna kit - by Bioz Stars, 2025-04
93/100 stars
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stbl3 competent cells  (tiangen biotech co)
93
tiangen biotech co stbl3 competent cells
Depletion of BRD4 via siBRD4 and JQ1 does not affect the transcriptional activity of AIRE. A, AIRE activates the human insulin promoter despite the depletion and inhibition of BRD4. Relative luciferase activities from hIns.Luc in the presence of mh.AIRE alone (bars 1–4) and with depleted BRD4 (bars 5–8), absent JQ1 (bars 1, 2, 5, and 6), and increasing amounts of JQ1 (bars 3, 4, 7, and 8) are presented as -fold activation above levels of the empty vector alone (bar 1). B, levels of informative proteins are presented in WBs. Levels of mh.AIRE (top panel), released BRD4 (second panel), total BRD4 (bottom panel, below the black line), and GAPDH (third panel) are presented. C, AIRE activates the KRT14 gene (AIRE-responsive) independently of BRD4. Relative RNA levels of KRT14 in the presence of mh.AIRE alone (bars 1–4) or with siBRD4 (bars 5–8) and increasing amounts of JQ1 (bars 3, 4, 7, and 8) or in the absence of JQ1 (bars 1, 2, 5, and 6) are presented as -fold activation above levels of the empty vector alone (bar 1). D, the CCNH gene (AIRE-nonresponsive) is not affected by AIRE or BRD4. Relative RNA levels are presented by the same analyses and conditions as in C. E–G, AIRE interacts with P-TEFb directly under physiological conditions. For AIRE immunoprecipitations (E), interactions between the ectopically expressed AIRE (bottom panel) and endogenous BRD4, <t>CycT1,</t> and CDK9 proteins (top to third panels) are presented in the presence or absence of siBRD4, compared with the IgG control (lane 1). CycT1 immunoprecipitations (F) were performed under the same conditions. Interactions between endogenous CycT1 (bottom panel) and BRD4, AIRE, and CDK9 (top to third panels) proteins are presented. G, WBs reveal levels of input proteins for E and F. Levels of total BRD4 (450 mm NaCl extraction) are presented in the bottom panel below the black line. For A, C, and D, error bars represent S.E., n = 3. Statistical significance is indicated by asterisks (paired t test; *, p < 0.05; **, p < 0.01).
Stbl3 Competent Cells, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stbl3 competent cells/product/tiangen biotech co
Average 93 stars, based on 1 article reviews
stbl3 competent cells - by Bioz Stars, 2025-04
93/100 stars
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Image Search Results


Depletion of BRD4 via siBRD4 and JQ1 does not affect the transcriptional activity of AIRE. A, AIRE activates the human insulin promoter despite the depletion and inhibition of BRD4. Relative luciferase activities from hIns.Luc in the presence of mh.AIRE alone (bars 1–4) and with depleted BRD4 (bars 5–8), absent JQ1 (bars 1, 2, 5, and 6), and increasing amounts of JQ1 (bars 3, 4, 7, and 8) are presented as -fold activation above levels of the empty vector alone (bar 1). B, levels of informative proteins are presented in WBs. Levels of mh.AIRE (top panel), released BRD4 (second panel), total BRD4 (bottom panel, below the black line), and GAPDH (third panel) are presented. C, AIRE activates the KRT14 gene (AIRE-responsive) independently of BRD4. Relative RNA levels of KRT14 in the presence of mh.AIRE alone (bars 1–4) or with siBRD4 (bars 5–8) and increasing amounts of JQ1 (bars 3, 4, 7, and 8) or in the absence of JQ1 (bars 1, 2, 5, and 6) are presented as -fold activation above levels of the empty vector alone (bar 1). D, the CCNH gene (AIRE-nonresponsive) is not affected by AIRE or BRD4. Relative RNA levels are presented by the same analyses and conditions as in C. E–G, AIRE interacts with P-TEFb directly under physiological conditions. For AIRE immunoprecipitations (E), interactions between the ectopically expressed AIRE (bottom panel) and endogenous BRD4, CycT1, and CDK9 proteins (top to third panels) are presented in the presence or absence of siBRD4, compared with the IgG control (lane 1). CycT1 immunoprecipitations (F) were performed under the same conditions. Interactions between endogenous CycT1 (bottom panel) and BRD4, AIRE, and CDK9 (top to third panels) proteins are presented. G, WBs reveal levels of input proteins for E and F. Levels of total BRD4 (450 mm NaCl extraction) are presented in the bottom panel below the black line. For A, C, and D, error bars represent S.E., n = 3. Statistical significance is indicated by asterisks (paired t test; *, p < 0.05; **, p < 0.01).

Journal: The Journal of Biological Chemistry

Article Title: Bromodomain-containing protein 4–independent transcriptional activation by autoimmune regulator (AIRE) and NF-κB

doi: 10.1074/jbc.RA117.001518

Figure Lengend Snippet: Depletion of BRD4 via siBRD4 and JQ1 does not affect the transcriptional activity of AIRE. A, AIRE activates the human insulin promoter despite the depletion and inhibition of BRD4. Relative luciferase activities from hIns.Luc in the presence of mh.AIRE alone (bars 1–4) and with depleted BRD4 (bars 5–8), absent JQ1 (bars 1, 2, 5, and 6), and increasing amounts of JQ1 (bars 3, 4, 7, and 8) are presented as -fold activation above levels of the empty vector alone (bar 1). B, levels of informative proteins are presented in WBs. Levels of mh.AIRE (top panel), released BRD4 (second panel), total BRD4 (bottom panel, below the black line), and GAPDH (third panel) are presented. C, AIRE activates the KRT14 gene (AIRE-responsive) independently of BRD4. Relative RNA levels of KRT14 in the presence of mh.AIRE alone (bars 1–4) or with siBRD4 (bars 5–8) and increasing amounts of JQ1 (bars 3, 4, 7, and 8) or in the absence of JQ1 (bars 1, 2, 5, and 6) are presented as -fold activation above levels of the empty vector alone (bar 1). D, the CCNH gene (AIRE-nonresponsive) is not affected by AIRE or BRD4. Relative RNA levels are presented by the same analyses and conditions as in C. E–G, AIRE interacts with P-TEFb directly under physiological conditions. For AIRE immunoprecipitations (E), interactions between the ectopically expressed AIRE (bottom panel) and endogenous BRD4, CycT1, and CDK9 proteins (top to third panels) are presented in the presence or absence of siBRD4, compared with the IgG control (lane 1). CycT1 immunoprecipitations (F) were performed under the same conditions. Interactions between endogenous CycT1 (bottom panel) and BRD4, AIRE, and CDK9 (top to third panels) proteins are presented. G, WBs reveal levels of input proteins for E and F. Levels of total BRD4 (450 mm NaCl extraction) are presented in the bottom panel below the black line. For A, C, and D, error bars represent S.E., n = 3. Statistical significance is indicated by asterisks (paired t test; *, p < 0.05; **, p < 0.01).

Article Snippet: The antibodies for WB, co-immunoprecipitation, and ChIP were as follows: AIRE (H300, sc-33188, Santa Cruz Biotechnology), FLAG (F3165, Sigma-Aldrich), Myc (9E10, ab32, Abcam), CycT1 (D1B6G, catalog no. 81464, Cell Signaling), CDK9 (C-20, sc-484, Santa Cruz Biotechnology), GAL4 (1–147, sc-4050, Santa Cruz Biotechnology), BRD4 (E2A7X, catalog no. 13440, Cell Signaling), GAPDH (GA1R, catalog no. MA5-15738, Invitrogen), RelA/p65 (D12E12, catalog no. 8242, Cell Signaling), ECL mouse IgG HRP-linked whole Ab (NA9310, GE Healthcare), ECL rabbit IgG HRP-linked whole Ab (NA9340, GE Healthcare), and rabbit and mouse control IgG (sc-2070 and sc-2050, Santa Cruz Biotechnology).

Techniques: Activity Assay, Inhibition, Luciferase, Activation Assay, Plasmid Preparation

Depletion of BRD4 by siBRD4 and inactivation by JQ1 do not disrupt interactions between P-TEFb and AIRE or p65. A–C, for AIRE immunoprecipitations (A), interactions between the ectopically expressed AIRE (bottom) and endogenous BRD4, CycT1, and CDK9 proteins (top to third panels) are presented, in the absence or presence of siBRD4 and JQ1, compared with the IgG control (lane 1). For CycT1 immunoprecipitations (B), under the same conditions, compared with IgG (lane 1), interactions between endogenous CycT1 (fourth panel) and BRD4, AIRE, and CDK9 (top to third panels) are presented. C, WBs reveal levels of input proteins for A and B. Under the same conditions, compared with the IgG control (lane 1), for p65 immunoprecipitations (D), interactions between ectopically expressed p65 (bottom panel) and endogenous BRD4, CycT1, and CDK9 proteins (top to third panels) are presented. For CycT1 immunoprecipitations (E), interactions between endogenous CycT1 (bottom panel) and BRD4, p65, and CDK9 (top to third panels) are presented. F, WBs reveal levels of input proteins for D and E.

Journal: The Journal of Biological Chemistry

Article Title: Bromodomain-containing protein 4–independent transcriptional activation by autoimmune regulator (AIRE) and NF-κB

doi: 10.1074/jbc.RA117.001518

Figure Lengend Snippet: Depletion of BRD4 by siBRD4 and inactivation by JQ1 do not disrupt interactions between P-TEFb and AIRE or p65. A–C, for AIRE immunoprecipitations (A), interactions between the ectopically expressed AIRE (bottom) and endogenous BRD4, CycT1, and CDK9 proteins (top to third panels) are presented, in the absence or presence of siBRD4 and JQ1, compared with the IgG control (lane 1). For CycT1 immunoprecipitations (B), under the same conditions, compared with IgG (lane 1), interactions between endogenous CycT1 (fourth panel) and BRD4, AIRE, and CDK9 (top to third panels) are presented. C, WBs reveal levels of input proteins for A and B. Under the same conditions, compared with the IgG control (lane 1), for p65 immunoprecipitations (D), interactions between ectopically expressed p65 (bottom panel) and endogenous BRD4, CycT1, and CDK9 proteins (top to third panels) are presented. For CycT1 immunoprecipitations (E), interactions between endogenous CycT1 (bottom panel) and BRD4, p65, and CDK9 (top to third panels) are presented. F, WBs reveal levels of input proteins for D and E.

Article Snippet: The antibodies for WB, co-immunoprecipitation, and ChIP were as follows: AIRE (H300, sc-33188, Santa Cruz Biotechnology), FLAG (F3165, Sigma-Aldrich), Myc (9E10, ab32, Abcam), CycT1 (D1B6G, catalog no. 81464, Cell Signaling), CDK9 (C-20, sc-484, Santa Cruz Biotechnology), GAL4 (1–147, sc-4050, Santa Cruz Biotechnology), BRD4 (E2A7X, catalog no. 13440, Cell Signaling), GAPDH (GA1R, catalog no. MA5-15738, Invitrogen), RelA/p65 (D12E12, catalog no. 8242, Cell Signaling), ECL mouse IgG HRP-linked whole Ab (NA9310, GE Healthcare), ECL rabbit IgG HRP-linked whole Ab (NA9340, GE Healthcare), and rabbit and mouse control IgG (sc-2070 and sc-2050, Santa Cruz Biotechnology).

Techniques:

BRD4 depletion has no effect on the recruitment of P-TEFb to AIRE or p65 in chromatin. A and B, for CycT1 (A) and AIRE (B) ChIPs, relative enrichment of target proteins at indicated promoters is presented: KRT14 (bars 1–4), S100A8 (bars 5–8), and IL-8 (bars 9–12) promoters. C and D, for CycT1 (C) and p65 (D) ChIPs, relative enrichment of target proteins at indicated promoters is presented: IL-8 (bars 1–4), TNFα (bars 5–8), and KRT14 (bars 9–12) promoters. E and F, WBs reveal levels of the indicated proteins for ChIPs. Relative enrichment of target proteins at chosen promoters is presented as -fold change compared with the sample with the highest value, which was set to 1. For A–D, error bars represent S.E., n = 3. Statistical significance is indicated by asterisks (paired t test; *, p < 0.05; **, p < 0.01).

Journal: The Journal of Biological Chemistry

Article Title: Bromodomain-containing protein 4–independent transcriptional activation by autoimmune regulator (AIRE) and NF-κB

doi: 10.1074/jbc.RA117.001518

Figure Lengend Snippet: BRD4 depletion has no effect on the recruitment of P-TEFb to AIRE or p65 in chromatin. A and B, for CycT1 (A) and AIRE (B) ChIPs, relative enrichment of target proteins at indicated promoters is presented: KRT14 (bars 1–4), S100A8 (bars 5–8), and IL-8 (bars 9–12) promoters. C and D, for CycT1 (C) and p65 (D) ChIPs, relative enrichment of target proteins at indicated promoters is presented: IL-8 (bars 1–4), TNFα (bars 5–8), and KRT14 (bars 9–12) promoters. E and F, WBs reveal levels of the indicated proteins for ChIPs. Relative enrichment of target proteins at chosen promoters is presented as -fold change compared with the sample with the highest value, which was set to 1. For A–D, error bars represent S.E., n = 3. Statistical significance is indicated by asterisks (paired t test; *, p < 0.05; **, p < 0.01).

Article Snippet: The antibodies for WB, co-immunoprecipitation, and ChIP were as follows: AIRE (H300, sc-33188, Santa Cruz Biotechnology), FLAG (F3165, Sigma-Aldrich), Myc (9E10, ab32, Abcam), CycT1 (D1B6G, catalog no. 81464, Cell Signaling), CDK9 (C-20, sc-484, Santa Cruz Biotechnology), GAL4 (1–147, sc-4050, Santa Cruz Biotechnology), BRD4 (E2A7X, catalog no. 13440, Cell Signaling), GAPDH (GA1R, catalog no. MA5-15738, Invitrogen), RelA/p65 (D12E12, catalog no. 8242, Cell Signaling), ECL mouse IgG HRP-linked whole Ab (NA9310, GE Healthcare), ECL rabbit IgG HRP-linked whole Ab (NA9340, GE Healthcare), and rabbit and mouse control IgG (sc-2070 and sc-2050, Santa Cruz Biotechnology).

Techniques: