Journal: Frontiers in Neuroscience

Article Title: Acute and chronic glucocorticoid treatments regulate astrocyte-enriched mRNAs in multiple brain regions in vivo

doi: 10.3389/fnins.2013.00139

Figure Lengend Snippet: mRNAs assessed for glucocorticoid regulation in the brain in vivo .

Article Snippet: Specific Taqman mRNA assays used in this analysis are: Actb (Mm01205647_g1), Adora2b (Mm00839292_m1), Aldh1l1 (Mm00550947_m1), Atp6v1b2 (Mm00431987_m1), Ch25h (Mm00515486_s1), Egr2 (Mm00456650_m1), Fgfr1 (Mm00438930_m1), Fgfr3 (Mm00433294_m1), Fkbp5 (Mm00487401_m1), Folh1 (Mm00489655_m1), Foxo1 (Mm00490672_m1), Gap43 (Mm00500404_m1), Gfap (Mm01253033_m1), Gja1 (Mm00439105_m1), Gjb6 (Mm01317508_m1), Glul (Mm00725701_s1), Hdac7 (Mm00469520_m1), Klf9 (Mm00495172_m1), Mapk4 (Mm00554001_m1), Mertk (Mm00434920_m1), Pdk4 (Mm01166879_m1), Per1 (Mm00501813_m1), Phlda1 (Mm00456345_g1), Prodh (Mm00448401_m1), Sgk1 (Mm00441380_m1), Slc1a2 (Mm00441457_m1), Slc1a3 (Mm00600697_m1), Sult1a1 (Mm01132072_m1), Syn2 (Mm00449780_m1), Txnip (Mm00452393_m1), Wnt7a (Mm00437354_m1).

Techniques: In Vivo, Binding Assay, Histone Deacetylase Assay

Journal: Foods

Article Title: Transcriptional Biomarkers and Immunohistochemistry for Detection of Illicit Dexamethasone Administration in Veal Calves

doi: 10.3390/foods11121810

Figure Lengend Snippet: List of selected targets for Gene expression study, reference sequences, amplicon sizes and supplier references for each Taqman assay. Reference gene for data normalisation marked with *.

Article Snippet: Bt03217338_m1 , SULT1A1 , NM_177521.2 , 73 bp.

Techniques: Gene Expression, Amplification, TaqMan Assay

Journal: Pharmacogenomics

Article Title: Multi-ethnic SULT1A1 copy number profiling with multiplex ligation-dependent probe amplification

doi: 10.2217/pgs-2018-0047

Figure Lengend Snippet: Multi-ethnic SULT1A1 copy number frequencies.

Article Snippet: MLPA analysis of SULT1A1 copy number was validated by commercially available TaqMan ® real-time qPCR Copy Number Assays that interrogated exon 1 and intron 2 of the minor transcript variant 5 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_177536.2","term_id":"214010150","term_text":"NM_177536.2"}} NM_177536.2 ) and the 3′UTR region of both major and minor SULT1A1 transcripts (Hs00736771_cn, Hs04461762_cn, Hs04461427_cn; Thermo Fisher Scientific) as per the manufacturer's instructions.

Techniques:

Journal: Aging (Albany NY)

Article Title: Overexpression of steroid sulfotransferase genes is associated with worsened prognosis and with immune exclusion in clear cell-renal cell carcinoma

doi: 10.18632/aging.102392

Figure Lengend Snippet: Overexpression of steroid SULT genes is associated with worsened prognosis in ccRCC. Reproduced from the TCGA KIRC dataset with cBioPortal platform, shown are ( A ) Percentage of ccRCC case with overexpression of steroid sulfotransferase (SULT) genes amongst 534 cases; ( B – C ) Progression-free and overall survival (OS) between ccRCC patients with steroid SULT genes upregulated and unchanged. Reproduced from TCGA KIRC dataset with Human Protein Atlas platform, shown are ( D ) OS of ccRCC patients with high or low mRNA level of SULT1A1, SULT1E1, SULT2A2 and SULT2B1 at automatically defined cutoffs. Reproduced from TCGA KIRC dataset with GEPIA and UALCAN platform, shown are ( E–H ) Contrast in gene expressions of steroid SULT genes between normal and cancer tissue, respectively (****P < 0.0001).

Article Snippet: The human SULT1A1, SULT1E1, SULT2A1 and SULT2B1 cDNA clones were obtained from Origene and a lentivirus-based vector was constructed as previously reported.

Techniques: Over Expression

Journal: Pharmacogenomics

Article Title: Multi-ethnic SULT1A1 copy number profiling with multiplex ligation-dependent probe amplification

doi: 10.2217/pgs-2018-0047

Figure Lengend Snippet: Multi-ethnic SULT1A1 copy number frequencies.

Article Snippet: Copy number analysis by qPCR MLPA analysis of SULT1A1 copy number was validated by commercially available TaqMan ® real-time qPCR Copy Number Assays that interrogated exon 1 and intron 2 of the minor transcript variant 5 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_177536.2","term_id":"214010150","term_text":"NM_177536.2"}} NM_177536.2 ) and the 3′UTR region of both major and minor SULT1A1 transcripts (Hs00736771_cn, Hs04461762_cn, Hs04461427_cn; Thermo Fisher Scientific) as per the manufacturer's instructions.

Techniques: