sucrose-infiltrated 2.1 m root tips Search Results


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  • 91
    Nanoprobes nanogold
    Immunogold labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced <t>Nanogold.</t> Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.
    Nanogold, supplied by Nanoprobes, used in various techniques. Bioz Stars score: 91/100, based on 623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Abcam rabbit anti gfp serum
    Immunogold Localization of <t>VHA-a1–GFP</t> and TGN Morphology.
    Rabbit Anti Gfp Serum, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nanoprobes goat fab anti rabbit igg
    Immunogold Localization of <t>VHA-a1–GFP</t> and TGN Morphology.
    Goat Fab Anti Rabbit Igg, supplied by Nanoprobes, used in various techniques. Bioz Stars score: 89/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore uranyl acetate methylcellulose
    Immunogold Localization of <t>VHA-a1–GFP</t> and TGN Morphology.
    Uranyl Acetate Methylcellulose, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam immunogold labeling
    <t>Immunogold</t> labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.
    Immunogold Labeling, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Merck & Co hexadecane
    <t>Immunogold</t> labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.
    Hexadecane, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co electron microscopy
    <t>Immunogold</t> labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.
    Electron Microscopy, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam rabbit anti gfp antibodies
    <t>Immunogold</t> labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.
    Rabbit Anti Gfp Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Nanoprobes silver enhanced
    <t>Immunogold</t> labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.
    Silver Enhanced, supplied by Nanoprobes, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co ultrastructural analysis
    <t>Immunogold</t> labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.
    Ultrastructural Analysis, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunogold labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.

    Journal: The Plant Journal

    Article Title: Function of the anion transporter AtCLC-d in the trans-Golgi network

    doi: 10.1111/j.1365-313X.2007.03061.x

    Figure Lengend Snippet: Immunogold labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.

    Article Snippet: Immunogold labeling was performed on ultra-thin (80–100 nm) thawed Tokuyasu cryosections of formaldehyde-fixed (8%, 2 h) and sucrose-infiltrated (2.1 M) root tips using rabbit anti-GFP serum (1:250, 60 min; Abcam, http://www.abcam.com ) and silver-enhanced (HQ Silver, 8 min; Nanoprobes, http://www.nanoprobes.com ) goat (Fab′) anti-rabbit IgG coupled to Nanogold (1:50; no. 2004, Nanoprobes) ( ).

    Techniques: Labeling, Stable Transfection, Expressing

    Immunogold Localization of VHA-a1–GFP and TGN Morphology.

    Journal: The Plant Cell

    Article Title: Vacuolar H+-ATPase Activity Is Required for Endocytic and Secretory Trafficking in Arabidopsis [W]

    doi: 10.1105/tpc.105.037978

    Figure Lengend Snippet: Immunogold Localization of VHA-a1–GFP and TGN Morphology.

    Article Snippet: Immunogold labeling was performed on ultrathin thawed Tokuyasu cryosections of formaldehyde-fixed (8%, 3 h) and sucrose-infiltrated (2.1 M) root tips using rabbit anti-GFP serum (1:25; Abcam) or rabbit anti-VHA-E serum (1:500) ( ) and silver-enhanced (HQ Silver, 6 min; Nanoprobes) goat (Fab′) anti-rabbit IgG coupled to Nanogold (No. 2004; Nanoprobes).

    Techniques:

    Rapid Colocalization of VHA-a1–GFP with FM4-64.

    Journal: The Plant Cell

    Article Title: Vacuolar H+-ATPase Activity Is Required for Endocytic and Secretory Trafficking in Arabidopsis [W]

    doi: 10.1105/tpc.105.037978

    Figure Lengend Snippet: Rapid Colocalization of VHA-a1–GFP with FM4-64.

    Article Snippet: Immunogold labeling was performed on ultrathin thawed Tokuyasu cryosections of formaldehyde-fixed (8%, 3 h) and sucrose-infiltrated (2.1 M) root tips using rabbit anti-GFP serum (1:25; Abcam) or rabbit anti-VHA-E serum (1:500) ( ) and silver-enhanced (HQ Silver, 6 min; Nanoprobes) goat (Fab′) anti-rabbit IgG coupled to Nanogold (No. 2004; Nanoprobes).

    Techniques:

    Subcellular Localization of VHA-a–GFP Fusion Proteins Expressed in Roots of Seedlings.

    Journal: The Plant Cell

    Article Title: Vacuolar H+-ATPase Activity Is Required for Endocytic and Secretory Trafficking in Arabidopsis [W]

    doi: 10.1105/tpc.105.037978

    Figure Lengend Snippet: Subcellular Localization of VHA-a–GFP Fusion Proteins Expressed in Roots of Seedlings.

    Article Snippet: Immunogold labeling was performed on ultrathin thawed Tokuyasu cryosections of formaldehyde-fixed (8%, 3 h) and sucrose-infiltrated (2.1 M) root tips using rabbit anti-GFP serum (1:25; Abcam) or rabbit anti-VHA-E serum (1:500) ( ) and silver-enhanced (HQ Silver, 6 min; Nanoprobes) goat (Fab′) anti-rabbit IgG coupled to Nanogold (No. 2004; Nanoprobes).

    Techniques:

    Immunogold labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.

    Journal: The Plant Journal

    Article Title: Function of the anion transporter AtCLC-d in the trans-Golgi network

    doi: 10.1111/j.1365-313X.2007.03061.x

    Figure Lengend Snippet: Immunogold labeling of ultra-thin root cryosections of plants stably expressing AtCLC-d–GFP. (a, b) Anti-GFP antibodies were detected with silver-enhanced Nanogold. Gold particles accumulated in the TGN region close to the trans -Golgi cisternae of cortex and epidermal cells of root tips. The trans-Golgi side is marked with (T) and is always shown on the lower side of the Golgi stack (G). (c) No labeling above background was seen in controls not expressing AtCLC-d–GFP. (d) Labeling in plants stably expressing VHA-a1–GFP. Scale bar = 200 nm.

    Article Snippet: Immunogold labeling was performed on ultra-thin (80–100 nm) thawed Tokuyasu cryosections of formaldehyde-fixed (8%, 2 h) and sucrose-infiltrated (2.1 M) root tips using rabbit anti-GFP serum (1:250, 60 min; Abcam, http://www.abcam.com ) and silver-enhanced (HQ Silver, 8 min; Nanoprobes, http://www.nanoprobes.com ) goat (Fab′) anti-rabbit IgG coupled to Nanogold (1:50; no. 2004, Nanoprobes) ( ).

    Techniques: Labeling, Stable Transfection, Expressing