Journal: Biosensors

Article Title: Ultrasensitive Label-Free Sensing of IL-6 Based on PASE Functionalized Carbon Nanotube Micro-Arrays with RNA-Aptamers as Molecular Recognition Elements

doi: 10.3390/bios7020017

Figure Lengend Snippet: Schematic showing the reaction of 1-Pyrenebutanoic Acid Succinimidyl Ester (PASE) onto IL-6 aptamer forming the IL-6 aptamer PASE complex. Three groups of devices were prepared, Carbon Nanotube (CNT) device functionalized with ( B ) IL-6 aptamer PASE complex, positive control; ( C ) IgG, negative control; and ( D ) tween-20 blocking agent, negative control.

Article Snippet: Device Functionalization CNT biosensors were functionalized with 1-Pyrenebutanoic Acid Succinimidyl Ester (PASE) conjugated IL-6 aptamers (Base Pair biotechnologies, Pearland, TX, USA; Cat.#: ATW0077) at 20 μg/mL in 1 mM MgCL2 1× PBS buffer solution for 2 h at room temperature.

Techniques: Positive Control, Negative Control, Blocking Assay