Journal: Chemical Biology & Drug Design

Article Title: Investigation on the antipyretic mechanism of Chaiqin Qingning capsule for the treatment of fever based on network pharmacology, molecular docking, and in vitro experimental validation

doi: 10.1111/cbdd.14451

Figure Lengend Snippet: FIGURE 7 Western blot detection of CQQNC on the expression of COX-1, COX-2, cAMP, EP3, and mPGES1. Statistical significance: ∆∆∆∆p < .0001, ∆∆p < .01, and ∆p < .05 represents IL-1β group versus control group. ****p < .0001, **p < .01, and *p < .05 represent CQQNC group versus IL-1β group (n = 3).

Article Snippet: AntiCOX- 1 (batch number: 00100314), COX- 2 (batch number: 10027896), EP3 (batch number: 00071265), mouse and rabbit GAPDH (batch number: 10021642 and 0013796) antibody were purchased from Proteintech.

Techniques: Western Blot, Expressing, Control

Journal: Cell communication and signaling : CCS

Article Title: PTGER4 signaling regulates class IIa HDAC function and SPINK4 mRNA levels in rectal epithelial cells.

doi: 10.1186/s12964-024-01879-1

Figure Lengend Snippet: Fig. 5 PGE2-PTGER4 activates HDACs to increase SPINK4 expression. Western blot analysis for A EP4i, B PGE2 or EP4i, or C butyrate-treated organoid protein lysates probed for HDAC4 (S246)/ HDAC5 (S259)/ HDAC7 (S155), HDAC 4, and GAPDH as a loading control. The experiment in panel A is representative of an experiment that was performed on 2 different patients. Panels B and C are representative of experiments performed on 3 different patient samples. D RT-PCR fold-change quantification of SPINK4 normalized to β-ACTIN in organoids treated with PGE2 and/or LMK-235 (n = 5 different patient samples were used in this experiment). ns = non-significant; *P < 0.05; **P < 0.01; Two-way ANOVA

Article Snippet: For detecting PTGER4 by Western blotting, rabbit anti-PTGER4 was obtained from Proteintech (cat. no. 24895–1-AP).

Techniques: Expressing, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction

Journal: Animals : an Open Access Journal from MDPI

Article Title: FABP3 Mediates Lipid Droplet Accumulation and Adhesive Capacity in Bovine Endometrial Epithelial Cells via PGE 2 /PTGER4/PPAR Axis

doi: 10.3390/ani15233417

Figure Lengend Snippet: PGE 2 binds to PTGER4 to regulate FABP3 expression in bEECs: ( A ) The difference in PTGER ( PTGER1 , PTGER2 , PTGER3 and PTGER4 ) expression in bEECs after treatment with P 4 and/or PGE 2 . ( B – D ) The representative images and analysis of PTGER2 and PTGER4 expression in bEECs after treatment with P 4 and/or PGE 2 . ( E , F ) The representative images and analysis of PTGER2 expression in bEECs between shN and shPTGER2 groups after treatment with DMSO or P 4 + PGE 2 . ( G , H ) The representative images and analysis of PTGER4 expression in bEECs between shN and shPTGER4 groups after treatment with DMSO or P 4 + PGE 2 . ( I ) The analysis of FABP3 expression in bEECs after treatment with DMSO, PGE 2 or the inhibitor of PTGER4 ( GW627368 ). The data are means ± SEM of three independent experiments. Statistically significant differences are indicated by asterisks: ** p < 0.01, ns p > 0.05. Bars with different letters are significantly different ( p < 0.05).

Article Snippet: Then, PGE 2 (10 −8 –10 −6 M; 14010, Cayman, Ann Arbor, MI, USA) was added to the medium, and the cells were cultured for 24 h. For inhibitor treatment, 5 and 10 μM PTGER2 inhibitor (TG-155; MedChemExpress, Newark, NJ, USA) or 5 and 10 μM PTGER4 inhibitor ( GW627368 ; Selleck, Houston, TX, USA) were added to the medium 1 h prior to the PGE 2 treatment.

Techniques: Expressing

Journal: Vaccine

Article Title: Fluorescence-barcoded cell lines stably expressing membrane-anchored influenza neuraminidases.

doi: 10.1016/j.vaccine.2025.127157

Figure Lengend Snippet: Fig. 1. Standard NA mAbs brightly and specifically label K530-NA cell lines. Shown are flow cytometry histograms depicting the binding of recombinant IgG versions of standard NA mAbs to K530 cell lines expressing membrane-anchored NAs. Each row corresponds to a monoclonal cell line stably expressing a single type of NA. Each column corresponds to the binding profile for a single mAb. MAbs were incubated with pooled cell lines comprising Option 1 or Option 2 (Table 1), and the resultant data were concatenated into a single figure.

Article Snippet: PBMCs in RPMI-1640 medium plus 10 % FBS were incubated with irrelevant mouse IgG1 (MG1K; Rockland) to block nonspecific binding and then labeled with fluorochrome-conjugated mAbs.

Techniques: Flow Cytometry, Binding Assay, Recombinant, Expressing, Membrane, Stable Transfection, Incubation

Journal: Vaccine

Article Title: Fluorescence-barcoded cell lines stably expressing membrane-anchored influenza neuraminidases.

doi: 10.1016/j.vaccine.2025.127157

Figure Lengend Snippet: Fig. 3. Use of K530-NA cells to determine the binding breadth of newly identified NA mAbs. Shown are flow cytometry histograms depicting the binding of re combinant IgG versions of NA mAbs from donors T1, T2, or T3 to K530 cell lines expressing membrane-anchored NAs, as in Fig. 1.

Article Snippet: PBMCs in RPMI-1640 medium plus 10 % FBS were incubated with irrelevant mouse IgG1 (MG1K; Rockland) to block nonspecific binding and then labeled with fluorochrome-conjugated mAbs.

Techniques: Binding Assay, Flow Cytometry, Expressing, Membrane

Journal: Vaccine

Article Title: Fluorescence-barcoded cell lines stably expressing membrane-anchored influenza neuraminidases.

doi: 10.1016/j.vaccine.2025.127157

Figure Lengend Snippet: Fig. 4. Analysis of serum IgGs elicited by infection of rhesus macaques with H3N2 influenza virus. A) Pre-immune (day 0) or immune (day 27 or 28) blood plasma from three rhesus macaques (6451, T651, T771) infected with A/Aichi/2/1968 (H3N2) influenza virus was incubated with K530-NA cell lines. The degree of IgG labeling of each cell line was determined by flow cytometry, as in Fig. 1. Vertical, black lines denote the threshold for specific labeling of cell-surface NA, determined according to the labeling intensity observed for K530 cells expressing no NA (top row). B) Fold-change in the fluorescence intensity of plasma IgG labeling of selected K530-N2 cell lines, after either infection with H3N2 virus (Fig. 4A) or immunization with recombinant H3 HA (Supplementary Fig. 3B). Fold-change was calculated as the ratio of the geometric mean fluorescence intensity (geoMFI) resulting from labeling with the immune plasma IgG, divided by the geoMFI resulting from labeling with pre-immune plasma IgG. Each symbol represents a single animal. The difference in group means was analyzed by two-tailed t-test with Welch’s correction, as described in Materials and Methods.

Article Snippet: PBMCs in RPMI-1640 medium plus 10 % FBS were incubated with irrelevant mouse IgG1 (MG1K; Rockland) to block nonspecific binding and then labeled with fluorochrome-conjugated mAbs.

Techniques: Infection, Virus, Clinical Proteomics, Incubation, Labeling, Flow Cytometry, Expressing, Fluorescence, Recombinant, Two Tailed Test