Journal: Human molecular genetics

Article Title: Multikinase activity of fibroblast growth factor receptor (FGFR) inhibitors SU5402, PD173074, AZD1480, AZD4547 and BGJ398 compromises the use of small chemicals targeting FGFR catalytic activity for therapy of short-stature syndromes.

doi: 10.1093/hmg/ddv441

Figure Lengend Snippet: Figure 1. TKIs rescue FGF2-mediated inhibition of chondrocyte proliferation. (A) RCS chondrocytes were treated with FGF2 and FGFR TKIs SU5402, PD173074, AZD1480, AZD4547 and BGJ398 as indicated, and cell numbers were determined by crystal violet staining 96 h later. Data represent averages for six wells. Results are

Article Snippet: The chemicals were obtained from the following manufacturers: FGF2 and FGF22 (R&D Systems, Minneapolis, MN); heparin (Sigma-Aldrich, St. Louis, MO); SU5402 and PD173074 (Tocris Bioscience, Bristol, UK); and AZD1480, AZD4547 and BGJ398 (Selleckchem, Houston, TX).

Techniques: Inhibition, Staining

Journal: Current Issues in Molecular Biology

Article Title: Pro-Angiogenetic Effects of Purified Extracts from Helix aspersa during Zebrafish Development

doi: 10.3390/cimb44080232

Figure Lengend Snippet: Pretreatment with a VEGF pathway inhibitor prevented the formation of intersomitic vessels: The panels show representative images of a WISH assay performed with fli1 as an endothelial marker, at 36 hpf, in untreated ( A , C , E , G , I ) and treated embryos ( B , D , F , H , J ) with SUGEN 5416. Previously, some embryos were treated with snail derivatives via Method I and controls with fish water and 0.1% DMSO (see Materials and Methods, and ). Magnification of the trunk region (32×). Two replicates were performed ( n = 15). Ratios at the bottom-left part of each picture specify the number of embryos showing the same staining pattern, compared to the total number of embryos used for each experiment. The images were taken in lateral position at 32× magnification with a Zeiss Axiozoom V13 (Zeiss, Jena, Germany) microscope, equipped with a PlanNeoFluar Z 1×/0.25 FWD 56 mm lens and Zen Pro software.

Article Snippet: Zebrafish embryos were then treated at 4 hpf with the snail derivatives LH, LM, LH3, and LM2, and then at 15 hpf with the VEGF inhibitor SU5416 (Sunitinib, Merck KGaA, Darmstadt, Germany) at a final concentration of 5 μg/mL in fish water and incubated in 3 cm diameter well-plates.

Techniques: Marker, Staining, Microscopy, Software