Journal:

Article Title: Human ?-Defensins HNPs-1, -2, and -3 in Renal Cell Carcinoma

doi:

Figure Lengend Snippet: RT-PCR analysis of HNP-1, -2, and -3 mRNA expression: 250 ng of cDNA from different cultured RCC lines, from the normal kidney cell line TK163, and PBMC of leukapheresis products were amplified for β-actin (A, control product, 317 bp) and HNP-1, -2, and -3 sequences (B, product 215 bp). Lane 1, Caki-2; lane 2, A-498; lane 3, ACHN; lane 4, 786-0; lane 5, 769-P; lane 6, LE 9211; lane 7, N43; lane 8, TW33; lane 9, PBMC; lane 10, TK163; lane 11, no cDNA.

Article Snippet: Cell Lines and Culture Conditions In this study, eight RCC lines were used that included five lines (A-498, Caki-2, 786-0, 769-P, ACHN) purchased from the American Type Culture Collection (ATCC; Manassas, VA), one line (LE 9211-RCC) obtained from Dr. B. van den Eynde (Ludwig Cancer Institute, Brussels, Belgium) and two lines (TW33, N43) were established from renal tumor tissues in our laboratory according to the previously described method.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Amplification

Journal:

Article Title: Human ?-Defensins HNPs-1, -2, and -3 in Renal Cell Carcinoma

doi:

Figure Lengend Snippet: Influences of HNP-1, -2, and -3 on cell proliferation of different RCC lines. A: RCC lines were incubated with and without different concentrations of the isolated HNP-1, -2, and -3 peptide fraction. Dose-dependent proliferative responses were measured by [3H]-TdR incorporation given as mean counts per minute (cpm) or as formazan-dye production in viable cells (A, inset); the RCC lines A-498 and ACHN showed a dose-dependent, significant increase of proliferative activity after stimulation with HNP-1, -2, and -3 in comparison to background proliferation in cultures without HNP-1, -2, and -3. Similar results were obtained in six independent stimulation experiments as well as with the synthetic HNP-1 peptide. B: Inhibition of cell proliferation of the RCC line A-498 stimulated with 12.5 μg/ml of isolated HNP-1, -2, and -3 is shown by preincubation of HNP-1, -2, and -3 with 12.5 μg/ml of the mAb DEF3 in comparison to cultures of the cell line incubated with defensins only (control of autocrine proliferation: incubation with RPMI 1640 medium only) C: Evaluation of total cell number by fluorescence microscopy after culture of the RCC line A-498 for 40 hours both with 12.5 μg/ml of isolated HNP-1, -2, and -3 and without. A significant increase of cell nuclei indicating cell proliferation was observed after stimulation with defensins.

Article Snippet: Cell Lines and Culture Conditions In this study, eight RCC lines were used that included five lines (A-498, Caki-2, 786-0, 769-P, ACHN) purchased from the American Type Culture Collection (ATCC; Manassas, VA), one line (LE 9211-RCC) obtained from Dr. B. van den Eynde (Ludwig Cancer Institute, Brussels, Belgium) and two lines (TW33, N43) were established from renal tumor tissues in our laboratory according to the previously described method.

Techniques: Incubation, Isolation, Activity Assay, Inhibition, Fluorescence, Microscopy

Journal: Sensors (Basel, Switzerland)

Article Title: Raman-Activated, Interactive Sorting of Isotope-Labeled Bacteria

doi: 10.3390/s24144503

Figure Lengend Snippet: ( A ) Raman mean spectra of single bacteria ( E. coli and M. lylae ) without isotope labeling (control, blue) and with labeling with D (red) or 13 C (green). ( B ) LD plot for the discrimination of different labeled bacteria with the subsequent loading vectors ( C ).

Article Snippet: For this study, the bacterial strains Escherichia coli ( E. coli ) DSM 498 and Micrococcus lylae ( M. lylae ) DSM 20,318 purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) were used.

Techniques: Bacteria, Quantitative Proteomics, Control, Labeling

Journal: Sensors (Basel, Switzerland)

Article Title: Raman-Activated, Interactive Sorting of Isotope-Labeled Bacteria

doi: 10.3390/s24144503

Figure Lengend Snippet: Unprocessed Raman spectra of single bacteria ( E. coli ) without isotope labeling (control, blue) and with labeling with D (red) or 13 C (green) measured in the microfluidic channel.

Article Snippet: For this study, the bacterial strains Escherichia coli ( E. coli ) DSM 498 and Micrococcus lylae ( M. lylae ) DSM 20,318 purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) were used.

Techniques: Bacteria, Quantitative Proteomics, Control, Labeling

Journal: Sensors (Basel, Switzerland)

Article Title: Raman-Activated, Interactive Sorting of Isotope-Labeled Bacteria

doi: 10.3390/s24144503

Figure Lengend Snippet: ( A ) Raman mean spectra of bacteria ( E. coli ) during sorting without isotope labeling (control, blue) and labeling with D (red) or 13 C (green) measured in the microfluidic channel. ( B ) LD plot for the discrimination of labeled and unlabeled bacteria.

Article Snippet: For this study, the bacterial strains Escherichia coli ( E. coli ) DSM 498 and Micrococcus lylae ( M. lylae ) DSM 20,318 purchased from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany) were used.

Techniques: Bacteria, Quantitative Proteomics, Control, Labeling