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carbon black  (Nanografi Advanced Materials)
93
Nanografi Advanced Materials carbon black
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rna cap structure analog  (New England Biolabs)
94
New England Biolabs rna cap structure analog
Rna Cap Structure Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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japan 4 w m keck structural biology laboratory  (Cold Spring Harbor Laboratory Meetings)
86
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fth1  (Proteintech)
93
Proteintech fth1
Fth1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sars cov 2  (Rockland Immunochemicals)
94
Rockland Immunochemicals sars cov 2
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m7g 5 ppp 5 g rna cap structure analog  (New England Biolabs)
95
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huwe1  (Proteintech)
93
Proteintech huwe1
Curcumol promotes <t>HUWE1-dependent</t> ubiquitin-mediated degradation of HK2 by inhibiting KAT8-mediated lactylation at the K346 site. A, D. Western blot analysis of HK2 protein half-life in LX2 cells treated with or without protein synthesis inhibitors in the presence of sodium lactate (10 mM, 24 h), with quantification of band intensity (n = 3). B. Western blot analysis of HK2 protein levels in LX2 cells transfected with LDHA siRNA or control siRNA and treated with chloroquine (CQ, 20 μg/mL), MG132 (10 μM), alone or in combination for 24 h (n = 3). C. Schematic diagram illustrating regulators of HK2 ubiquitination. E. Co-immunoprecipitation (Co-IP) analysis of the interaction between HK2 and the E3 ubiquitin ligase HUWE1. F. Western blot analysis of HK2 protein levels in LX2 cells treated with MG149 (50 μM, 48 h), with or without MG132 (10 μM, 6 h), followed by densitometric quantification (n = 3). G. Western blot analysis of HK2 protein levels in LX2 cells expressing HK2-WT or HK2-K346A after treatment with MG132 (10 μM, 8 h) (n = 3). H. Co-IP analysis of HK2 ubiquitination in LX2 cells with or without HUWE1 siRNA transfection (24 h). I. Co-IP analysis of HK2 ubiquitination in LX2 cells transfected with or without KAT8 siRNA (24 h) and treated with Curcumol (30 μM). J. Co-IP analysis was used to examine the interaction between HK2 and HUWE1 in LX2 cells that were co-transfected with the indicated plasmids and treated with Curcumol (30 μM, 8 h). K. Co-IP analysis of HK2 ubiquitination in LX2 cells expressing HK2-WT or HK2-K346A. L. Immunofluorescence analysis of HK2 (green), KAT8 (red), and HUWE1 (yellow) colocalization in liver tissues from CCl₄-induced fibrotic mice and Curcumol-treated mice (n = 3; scale bar: 50 μm). M. Co-IP analysis was performed on the co-transfected plasmids for 24 hours, and then the interactions between HK2, KAT8, and HUWE1 in LX2 cells treated with different concentrations of Curcumol (0, 20, 30, 45 μM) were examined. N, Q. Western blot analysis of HK2 degradation kinetics in LX2 cells expressing HK2-WT or HK2-K346A following HUWE1 siRNA transfection (24 h) and cycloheximide (CHX, 20 μg/mL) treatment for the indicated times (n = 3). O, P. Western blot analysis of HK2 protein levels in LX2 cells transfected with HUWE1 siRNA (24 h), with densitometric quantification (n = 3). Data are shown as mean ± SD, and statistical differences were analyzed by one-way ANOVA. ns indicates no significance; *p < 0.05, **p < 0.01, ***p < 0.001.
Huwe1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteintech 12693 1 ap ift172  (Proteintech)
93
Proteintech proteintech 12693 1 ap ift172
Curcumol promotes <t>HUWE1-dependent</t> ubiquitin-mediated degradation of HK2 by inhibiting KAT8-mediated lactylation at the K346 site. A, D. Western blot analysis of HK2 protein half-life in LX2 cells treated with or without protein synthesis inhibitors in the presence of sodium lactate (10 mM, 24 h), with quantification of band intensity (n = 3). B. Western blot analysis of HK2 protein levels in LX2 cells transfected with LDHA siRNA or control siRNA and treated with chloroquine (CQ, 20 μg/mL), MG132 (10 μM), alone or in combination for 24 h (n = 3). C. Schematic diagram illustrating regulators of HK2 ubiquitination. E. Co-immunoprecipitation (Co-IP) analysis of the interaction between HK2 and the E3 ubiquitin ligase HUWE1. F. Western blot analysis of HK2 protein levels in LX2 cells treated with MG149 (50 μM, 48 h), with or without MG132 (10 μM, 6 h), followed by densitometric quantification (n = 3). G. Western blot analysis of HK2 protein levels in LX2 cells expressing HK2-WT or HK2-K346A after treatment with MG132 (10 μM, 8 h) (n = 3). H. Co-IP analysis of HK2 ubiquitination in LX2 cells with or without HUWE1 siRNA transfection (24 h). I. Co-IP analysis of HK2 ubiquitination in LX2 cells transfected with or without KAT8 siRNA (24 h) and treated with Curcumol (30 μM). J. Co-IP analysis was used to examine the interaction between HK2 and HUWE1 in LX2 cells that were co-transfected with the indicated plasmids and treated with Curcumol (30 μM, 8 h). K. Co-IP analysis of HK2 ubiquitination in LX2 cells expressing HK2-WT or HK2-K346A. L. Immunofluorescence analysis of HK2 (green), KAT8 (red), and HUWE1 (yellow) colocalization in liver tissues from CCl₄-induced fibrotic mice and Curcumol-treated mice (n = 3; scale bar: 50 μm). M. Co-IP analysis was performed on the co-transfected plasmids for 24 hours, and then the interactions between HK2, KAT8, and HUWE1 in LX2 cells treated with different concentrations of Curcumol (0, 20, 30, 45 μM) were examined. N, Q. Western blot analysis of HK2 degradation kinetics in LX2 cells expressing HK2-WT or HK2-K346A following HUWE1 siRNA transfection (24 h) and cycloheximide (CHX, 20 μg/mL) treatment for the indicated times (n = 3). O, P. Western blot analysis of HK2 protein levels in LX2 cells transfected with HUWE1 siRNA (24 h), with densitometric quantification (n = 3). Data are shown as mean ± SD, and statistical differences were analyzed by one-way ANOVA. ns indicates no significance; *p < 0.05, **p < 0.01, ***p < 0.001.
Proteintech 12693 1 Ap Ift172, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rna cap analog  (New England Biolabs)
95
New England Biolabs rna cap analog
Curcumol promotes <t>HUWE1-dependent</t> ubiquitin-mediated degradation of HK2 by inhibiting KAT8-mediated lactylation at the K346 site. A, D. Western blot analysis of HK2 protein half-life in LX2 cells treated with or without protein synthesis inhibitors in the presence of sodium lactate (10 mM, 24 h), with quantification of band intensity (n = 3). B. Western blot analysis of HK2 protein levels in LX2 cells transfected with LDHA siRNA or control siRNA and treated with chloroquine (CQ, 20 μg/mL), MG132 (10 μM), alone or in combination for 24 h (n = 3). C. Schematic diagram illustrating regulators of HK2 ubiquitination. E. Co-immunoprecipitation (Co-IP) analysis of the interaction between HK2 and the E3 ubiquitin ligase HUWE1. F. Western blot analysis of HK2 protein levels in LX2 cells treated with MG149 (50 μM, 48 h), with or without MG132 (10 μM, 6 h), followed by densitometric quantification (n = 3). G. Western blot analysis of HK2 protein levels in LX2 cells expressing HK2-WT or HK2-K346A after treatment with MG132 (10 μM, 8 h) (n = 3). H. Co-IP analysis of HK2 ubiquitination in LX2 cells with or without HUWE1 siRNA transfection (24 h). I. Co-IP analysis of HK2 ubiquitination in LX2 cells transfected with or without KAT8 siRNA (24 h) and treated with Curcumol (30 μM). J. Co-IP analysis was used to examine the interaction between HK2 and HUWE1 in LX2 cells that were co-transfected with the indicated plasmids and treated with Curcumol (30 μM, 8 h). K. Co-IP analysis of HK2 ubiquitination in LX2 cells expressing HK2-WT or HK2-K346A. L. Immunofluorescence analysis of HK2 (green), KAT8 (red), and HUWE1 (yellow) colocalization in liver tissues from CCl₄-induced fibrotic mice and Curcumol-treated mice (n = 3; scale bar: 50 μm). M. Co-IP analysis was performed on the co-transfected plasmids for 24 hours, and then the interactions between HK2, KAT8, and HUWE1 in LX2 cells treated with different concentrations of Curcumol (0, 20, 30, 45 μM) were examined. N, Q. Western blot analysis of HK2 degradation kinetics in LX2 cells expressing HK2-WT or HK2-K346A following HUWE1 siRNA transfection (24 h) and cycloheximide (CHX, 20 μg/mL) treatment for the indicated times (n = 3). O, P. Western blot analysis of HK2 protein levels in LX2 cells transfected with HUWE1 siRNA (24 h), with densitometric quantification (n = 3). Data are shown as mean ± SD, and statistical differences were analyzed by one-way ANOVA. ns indicates no significance; *p < 0.05, **p < 0.01, ***p < 0.001.
Rna Cap Analog, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnastar lasergene12 software  (DNASTAR)
91
DNASTAR dnastar lasergene12 software
Curcumol promotes <t>HUWE1-dependent</t> ubiquitin-mediated degradation of HK2 by inhibiting KAT8-mediated lactylation at the K346 site. A, D. Western blot analysis of HK2 protein half-life in LX2 cells treated with or without protein synthesis inhibitors in the presence of sodium lactate (10 mM, 24 h), with quantification of band intensity (n = 3). B. Western blot analysis of HK2 protein levels in LX2 cells transfected with LDHA siRNA or control siRNA and treated with chloroquine (CQ, 20 μg/mL), MG132 (10 μM), alone or in combination for 24 h (n = 3). C. Schematic diagram illustrating regulators of HK2 ubiquitination. E. Co-immunoprecipitation (Co-IP) analysis of the interaction between HK2 and the E3 ubiquitin ligase HUWE1. F. Western blot analysis of HK2 protein levels in LX2 cells treated with MG149 (50 μM, 48 h), with or without MG132 (10 μM, 6 h), followed by densitometric quantification (n = 3). G. Western blot analysis of HK2 protein levels in LX2 cells expressing HK2-WT or HK2-K346A after treatment with MG132 (10 μM, 8 h) (n = 3). H. Co-IP analysis of HK2 ubiquitination in LX2 cells with or without HUWE1 siRNA transfection (24 h). I. Co-IP analysis of HK2 ubiquitination in LX2 cells transfected with or without KAT8 siRNA (24 h) and treated with Curcumol (30 μM). J. Co-IP analysis was used to examine the interaction between HK2 and HUWE1 in LX2 cells that were co-transfected with the indicated plasmids and treated with Curcumol (30 μM, 8 h). K. Co-IP analysis of HK2 ubiquitination in LX2 cells expressing HK2-WT or HK2-K346A. L. Immunofluorescence analysis of HK2 (green), KAT8 (red), and HUWE1 (yellow) colocalization in liver tissues from CCl₄-induced fibrotic mice and Curcumol-treated mice (n = 3; scale bar: 50 μm). M. Co-IP analysis was performed on the co-transfected plasmids for 24 hours, and then the interactions between HK2, KAT8, and HUWE1 in LX2 cells treated with different concentrations of Curcumol (0, 20, 30, 45 μM) were examined. N, Q. Western blot analysis of HK2 degradation kinetics in LX2 cells expressing HK2-WT or HK2-K346A following HUWE1 siRNA transfection (24 h) and cycloheximide (CHX, 20 μg/mL) treatment for the indicated times (n = 3). O, P. Western blot analysis of HK2 protein levels in LX2 cells transfected with HUWE1 siRNA (24 h), with densitometric quantification (n = 3). Data are shown as mean ± SD, and statistical differences were analyzed by one-way ANOVA. ns indicates no significance; *p < 0.05, **p < 0.01, ***p < 0.001.
Dnastar Lasergene12 Software, supplied by DNASTAR, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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m7gpppa  (New England Biolabs)
95
New England Biolabs m7gpppa
Curcumol promotes <t>HUWE1-dependent</t> ubiquitin-mediated degradation of HK2 by inhibiting KAT8-mediated lactylation at the K346 site. A, D. Western blot analysis of HK2 protein half-life in LX2 cells treated with or without protein synthesis inhibitors in the presence of sodium lactate (10 mM, 24 h), with quantification of band intensity (n = 3). B. Western blot analysis of HK2 protein levels in LX2 cells transfected with LDHA siRNA or control siRNA and treated with chloroquine (CQ, 20 μg/mL), MG132 (10 μM), alone or in combination for 24 h (n = 3). C. Schematic diagram illustrating regulators of HK2 ubiquitination. E. Co-immunoprecipitation (Co-IP) analysis of the interaction between HK2 and the E3 ubiquitin ligase HUWE1. F. Western blot analysis of HK2 protein levels in LX2 cells treated with MG149 (50 μM, 48 h), with or without MG132 (10 μM, 6 h), followed by densitometric quantification (n = 3). G. Western blot analysis of HK2 protein levels in LX2 cells expressing HK2-WT or HK2-K346A after treatment with MG132 (10 μM, 8 h) (n = 3). H. Co-IP analysis of HK2 ubiquitination in LX2 cells with or without HUWE1 siRNA transfection (24 h). I. Co-IP analysis of HK2 ubiquitination in LX2 cells transfected with or without KAT8 siRNA (24 h) and treated with Curcumol (30 μM). J. Co-IP analysis was used to examine the interaction between HK2 and HUWE1 in LX2 cells that were co-transfected with the indicated plasmids and treated with Curcumol (30 μM, 8 h). K. Co-IP analysis of HK2 ubiquitination in LX2 cells expressing HK2-WT or HK2-K346A. L. Immunofluorescence analysis of HK2 (green), KAT8 (red), and HUWE1 (yellow) colocalization in liver tissues from CCl₄-induced fibrotic mice and Curcumol-treated mice (n = 3; scale bar: 50 μm). M. Co-IP analysis was performed on the co-transfected plasmids for 24 hours, and then the interactions between HK2, KAT8, and HUWE1 in LX2 cells treated with different concentrations of Curcumol (0, 20, 30, 45 μM) were examined. N, Q. Western blot analysis of HK2 degradation kinetics in LX2 cells expressing HK2-WT or HK2-K346A following HUWE1 siRNA transfection (24 h) and cycloheximide (CHX, 20 μg/mL) treatment for the indicated times (n = 3). O, P. Western blot analysis of HK2 protein levels in LX2 cells transfected with HUWE1 siRNA (24 h), with densitometric quantification (n = 3). Data are shown as mean ± SD, and statistical differences were analyzed by one-way ANOVA. ns indicates no significance; *p < 0.05, **p < 0.01, ***p < 0.001.
M7gpppa, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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arca  (New England Biolabs)
95
New England Biolabs arca
Curcumol promotes <t>HUWE1-dependent</t> ubiquitin-mediated degradation of HK2 by inhibiting KAT8-mediated lactylation at the K346 site. A, D. Western blot analysis of HK2 protein half-life in LX2 cells treated with or without protein synthesis inhibitors in the presence of sodium lactate (10 mM, 24 h), with quantification of band intensity (n = 3). B. Western blot analysis of HK2 protein levels in LX2 cells transfected with LDHA siRNA or control siRNA and treated with chloroquine (CQ, 20 μg/mL), MG132 (10 μM), alone or in combination for 24 h (n = 3). C. Schematic diagram illustrating regulators of HK2 ubiquitination. E. Co-immunoprecipitation (Co-IP) analysis of the interaction between HK2 and the E3 ubiquitin ligase HUWE1. F. Western blot analysis of HK2 protein levels in LX2 cells treated with MG149 (50 μM, 48 h), with or without MG132 (10 μM, 6 h), followed by densitometric quantification (n = 3). G. Western blot analysis of HK2 protein levels in LX2 cells expressing HK2-WT or HK2-K346A after treatment with MG132 (10 μM, 8 h) (n = 3). H. Co-IP analysis of HK2 ubiquitination in LX2 cells with or without HUWE1 siRNA transfection (24 h). I. Co-IP analysis of HK2 ubiquitination in LX2 cells transfected with or without KAT8 siRNA (24 h) and treated with Curcumol (30 μM). J. Co-IP analysis was used to examine the interaction between HK2 and HUWE1 in LX2 cells that were co-transfected with the indicated plasmids and treated with Curcumol (30 μM, 8 h). K. Co-IP analysis of HK2 ubiquitination in LX2 cells expressing HK2-WT or HK2-K346A. L. Immunofluorescence analysis of HK2 (green), KAT8 (red), and HUWE1 (yellow) colocalization in liver tissues from CCl₄-induced fibrotic mice and Curcumol-treated mice (n = 3; scale bar: 50 μm). M. Co-IP analysis was performed on the co-transfected plasmids for 24 hours, and then the interactions between HK2, KAT8, and HUWE1 in LX2 cells treated with different concentrations of Curcumol (0, 20, 30, 45 μM) were examined. N, Q. Western blot analysis of HK2 degradation kinetics in LX2 cells expressing HK2-WT or HK2-K346A following HUWE1 siRNA transfection (24 h) and cycloheximide (CHX, 20 μg/mL) treatment for the indicated times (n = 3). O, P. Western blot analysis of HK2 protein levels in LX2 cells transfected with HUWE1 siRNA (24 h), with densitometric quantification (n = 3). Data are shown as mean ± SD, and statistical differences were analyzed by one-way ANOVA. ns indicates no significance; *p < 0.05, **p < 0.01, ***p < 0.001.
Arca, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Curcumol promotes HUWE1-dependent ubiquitin-mediated degradation of HK2 by inhibiting KAT8-mediated lactylation at the K346 site. A, D. Western blot analysis of HK2 protein half-life in LX2 cells treated with or without protein synthesis inhibitors in the presence of sodium lactate (10 mM, 24 h), with quantification of band intensity (n = 3). B. Western blot analysis of HK2 protein levels in LX2 cells transfected with LDHA siRNA or control siRNA and treated with chloroquine (CQ, 20 μg/mL), MG132 (10 μM), alone or in combination for 24 h (n = 3). C. Schematic diagram illustrating regulators of HK2 ubiquitination. E. Co-immunoprecipitation (Co-IP) analysis of the interaction between HK2 and the E3 ubiquitin ligase HUWE1. F. Western blot analysis of HK2 protein levels in LX2 cells treated with MG149 (50 μM, 48 h), with or without MG132 (10 μM, 6 h), followed by densitometric quantification (n = 3). G. Western blot analysis of HK2 protein levels in LX2 cells expressing HK2-WT or HK2-K346A after treatment with MG132 (10 μM, 8 h) (n = 3). H. Co-IP analysis of HK2 ubiquitination in LX2 cells with or without HUWE1 siRNA transfection (24 h). I. Co-IP analysis of HK2 ubiquitination in LX2 cells transfected with or without KAT8 siRNA (24 h) and treated with Curcumol (30 μM). J. Co-IP analysis was used to examine the interaction between HK2 and HUWE1 in LX2 cells that were co-transfected with the indicated plasmids and treated with Curcumol (30 μM, 8 h). K. Co-IP analysis of HK2 ubiquitination in LX2 cells expressing HK2-WT or HK2-K346A. L. Immunofluorescence analysis of HK2 (green), KAT8 (red), and HUWE1 (yellow) colocalization in liver tissues from CCl₄-induced fibrotic mice and Curcumol-treated mice (n = 3; scale bar: 50 μm). M. Co-IP analysis was performed on the co-transfected plasmids for 24 hours, and then the interactions between HK2, KAT8, and HUWE1 in LX2 cells treated with different concentrations of Curcumol (0, 20, 30, 45 μM) were examined. N, Q. Western blot analysis of HK2 degradation kinetics in LX2 cells expressing HK2-WT or HK2-K346A following HUWE1 siRNA transfection (24 h) and cycloheximide (CHX, 20 μg/mL) treatment for the indicated times (n = 3). O, P. Western blot analysis of HK2 protein levels in LX2 cells transfected with HUWE1 siRNA (24 h), with densitometric quantification (n = 3). Data are shown as mean ± SD, and statistical differences were analyzed by one-way ANOVA. ns indicates no significance; *p < 0.05, **p < 0.01, ***p < 0.001.

Journal: International Journal of Biological Sciences

Article Title: Curcumol Induces Necroptosis of Hepatic Stellate Cells by Targeting KAT8 to Suppress HK2 Lactylation and Promote HUWE1-Dependent Ubiquitination

doi: 10.7150/ijbs.125009

Figure Lengend Snippet: Curcumol promotes HUWE1-dependent ubiquitin-mediated degradation of HK2 by inhibiting KAT8-mediated lactylation at the K346 site. A, D. Western blot analysis of HK2 protein half-life in LX2 cells treated with or without protein synthesis inhibitors in the presence of sodium lactate (10 mM, 24 h), with quantification of band intensity (n = 3). B. Western blot analysis of HK2 protein levels in LX2 cells transfected with LDHA siRNA or control siRNA and treated with chloroquine (CQ, 20 μg/mL), MG132 (10 μM), alone or in combination for 24 h (n = 3). C. Schematic diagram illustrating regulators of HK2 ubiquitination. E. Co-immunoprecipitation (Co-IP) analysis of the interaction between HK2 and the E3 ubiquitin ligase HUWE1. F. Western blot analysis of HK2 protein levels in LX2 cells treated with MG149 (50 μM, 48 h), with or without MG132 (10 μM, 6 h), followed by densitometric quantification (n = 3). G. Western blot analysis of HK2 protein levels in LX2 cells expressing HK2-WT or HK2-K346A after treatment with MG132 (10 μM, 8 h) (n = 3). H. Co-IP analysis of HK2 ubiquitination in LX2 cells with or without HUWE1 siRNA transfection (24 h). I. Co-IP analysis of HK2 ubiquitination in LX2 cells transfected with or without KAT8 siRNA (24 h) and treated with Curcumol (30 μM). J. Co-IP analysis was used to examine the interaction between HK2 and HUWE1 in LX2 cells that were co-transfected with the indicated plasmids and treated with Curcumol (30 μM, 8 h). K. Co-IP analysis of HK2 ubiquitination in LX2 cells expressing HK2-WT or HK2-K346A. L. Immunofluorescence analysis of HK2 (green), KAT8 (red), and HUWE1 (yellow) colocalization in liver tissues from CCl₄-induced fibrotic mice and Curcumol-treated mice (n = 3; scale bar: 50 μm). M. Co-IP analysis was performed on the co-transfected plasmids for 24 hours, and then the interactions between HK2, KAT8, and HUWE1 in LX2 cells treated with different concentrations of Curcumol (0, 20, 30, 45 μM) were examined. N, Q. Western blot analysis of HK2 degradation kinetics in LX2 cells expressing HK2-WT or HK2-K346A following HUWE1 siRNA transfection (24 h) and cycloheximide (CHX, 20 μg/mL) treatment for the indicated times (n = 3). O, P. Western blot analysis of HK2 protein levels in LX2 cells transfected with HUWE1 siRNA (24 h), with densitometric quantification (n = 3). Data are shown as mean ± SD, and statistical differences were analyzed by one-way ANOVA. ns indicates no significance; *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The antibodies employed in this study include: RIPK1 (17519-1-AP), RIPK3 (17563-1-AP), P-RIPK1 (66854-1-Ig), MLKL (21066-1-AP), P-MLKL (82090-2-RR), HK2 (22029-1-AP), PKM2 (15822-1-AP), LDHA (19987-1-AP), PFKM (55028-1-AP), HUWE1 (19430-1-AP), Ubiquitin (10201-2-AP), HA (51064-2-AP), His (66005-1-Ig), EP300 (20695-1-AP) and P62 (18420-1-AP) from proteintech; KAT8 (sc-271691) from Santa Cruz Biotechnology; KAT6A from Bioswamp; Collagen I (ab26003), β-actin (ab8226), Tubulin (Ab721), anti-mouse IgG (ab190475), anti-rabbit IgG (ab288151) and LC3B (ab192890) from Abcam; Pan-Kla (AB_2868521) from PTM-Bio laboratory; KAT6B(A17116), P-RIPK3(AP1260), AARS(A15017), and AARS2 (A7826) from Abclonal.

Techniques: Ubiquitin Proteomics, Western Blot, Transfection, Control, Immunoprecipitation, Co-Immunoprecipitation Assay, Expressing, Immunofluorescence