stresnil Search Results


avetlabs n a azaperone chiron 13849 19 optixcare eye lube aventix animal health opx 4242 lidocaine hci injection with preservative  (ZeptoMetrix corporation)
93
ZeptoMetrix corporation avetlabs n a azaperone chiron 13849 19 optixcare eye lube aventix animal health opx 4242 lidocaine hci injection with preservative
Avetlabs N A Azaperone Chiron 13849 19 Optixcare Eye Lube Aventix Animal Health Opx 4242 Lidocaine Hci Injection With Preservative, supplied by ZeptoMetrix corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/avetlabs n a azaperone chiron 13849 19 optixcare eye lube aventix animal health opx 4242 lidocaine hci injection with preservative/product/ZeptoMetrix corporation
Average 93 stars, based on 1 article reviews
avetlabs n a azaperone chiron 13849 19 optixcare eye lube aventix animal health opx 4242 lidocaine hci injection with preservative - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier
azaperone  (Selleck Chemicals)
90
Selleck Chemicals azaperone
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Azaperone, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/azaperone/product/Selleck Chemicals
Average 90 stars, based on 1 article reviews
azaperone - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
azaperone stresniltm  (Janssen)
90
Janssen azaperone stresniltm
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Azaperone Stresniltm, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/azaperone stresniltm/product/Janssen
Average 90 stars, based on 1 article reviews
azaperone stresniltm - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
azaperone stresnil  (Elanco Inc)
90
Elanco Inc azaperone stresnil
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Azaperone Stresnil, supplied by Elanco Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/azaperone stresnil/product/Elanco Inc
Average 90 stars, based on 1 article reviews
azaperone stresnil - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
azaperon stresnil  (Janssen)
90
Janssen azaperon stresnil
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Azaperon Stresnil, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/azaperon stresnil/product/Janssen
Average 90 stars, based on 1 article reviews
azaperon stresnil - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
stresnil 1 mg/kg of body weight  (Janssen)
90
Janssen stresnil 1 mg/kg of body weight
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Stresnil 1 Mg/Kg Of Body Weight, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stresnil 1 mg/kg of body weight/product/Janssen
Average 90 stars, based on 1 article reviews
stresnil 1 mg/kg of body weight - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
stresnil  (Janssen)
90
Janssen stresnil
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Stresnil, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stresnil/product/Janssen
Average 90 stars, based on 1 article reviews
stresnil - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
stresnil neuroleptic injection for pigs  (Ausrichter Pty Ltd)
90
Ausrichter Pty Ltd stresnil neuroleptic injection for pigs
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Stresnil Neuroleptic Injection For Pigs, supplied by Ausrichter Pty Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stresnil neuroleptic injection for pigs/product/Ausrichter Pty Ltd
Average 90 stars, based on 1 article reviews
stresnil neuroleptic injection for pigs - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
azaperone stresnil  (Eli Lilly)
90
Eli Lilly azaperone stresnil
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Azaperone Stresnil, supplied by Eli Lilly, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/azaperone stresnil/product/Eli Lilly
Average 90 stars, based on 1 article reviews
azaperone stresnil - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
azaperone and metomidate stresnil and hypnodil  (Janssen)
90
Janssen azaperone and metomidate stresnil and hypnodil
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Azaperone And Metomidate Stresnil And Hypnodil, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/azaperone and metomidate stresnil and hypnodil/product/Janssen
Average 90 stars, based on 1 article reviews
azaperone and metomidate stresnil and hypnodil - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
azaperone stresnil  (Ecuphar GmbH)
90
Ecuphar GmbH azaperone stresnil
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Azaperone Stresnil, supplied by Ecuphar GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/azaperone stresnil/product/Ecuphar GmbH
Average 90 stars, based on 1 article reviews
azaperone stresnil - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
azoperon stresnil  (Janssen)
90
Janssen azoperon stresnil
Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, <t>azaperone,</t> or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.
Azoperon Stresnil, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/azoperon stresnil/product/Janssen
Average 90 stars, based on 1 article reviews
azoperon stresnil - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, azaperone, or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.

Journal: Molecular pharmacology

Article Title: A High-Throughput Screening Assay to Identify Drugs that Can Treat Long QT Syndrome Caused by Trafficking-Deficient K V 11.1 (hERG) Variants.

doi: 10.1124/molpharm.121.000421

Figure Lengend Snippet: Fig. 8. Optimized Tl1 flux assay detected increased KV11.1-G601S-G965*X trafficking with three known KV11.1 inhibitors. (A) Bar graph show- ing Tl1 flux Z scores after 24-hour treatment with E-4031. 56 wells were selected from central rows of the plate as representative of control. Dot- ted black line indicates threshold for “hit” selection set to Z score $ 3. (B) Western blot showing increased fully glycosylated protein (mature), representative of increased KV11.1 protein trafficking after 24-hour treatment with 10 mM azelastine, azaperone, or ibutilide. All wells loaded with 20 mg of protein. (C) Western blot showing increased KV11.1 protein trafficking after 24 hours of treatment with 10 mM azelastine, azaper- one, or ibutilide in HEK-293 cells expressing KV11.1-N470D and KV11.1-G601S. All wells loaded with 20 mg of protein. (D) Whole cell current recordings at 120 mV step. Representative current traces (left) and bar graphs (right) depicting KV11.1-G601S-G965*X current after 24-hour incubation with 10 mM drug and washout before experiments. Inset shows enlarged current traces at 120 mV voltage step. N 5 8 for E-4031 treatment and n 5 7 for all other treatments. Treated samples compared with vehicle control (0.1% DMSO) by one-way ANOVA with post hoc Dunnett’s t test. Bars on scatter plots represent mean of each group. * 5 P < 0.05. Veh. 5 0.1% DMSO, E-4. 5 E-4031, Azel. 5 azelastine, Azap. 5 azaperone, and Ibu. 5 ibutilide.

Article Snippet: Ibutilide, gabapentin, and azaperone were purchased from SelleckChem, Azelastine and VU0405601 were purchased from Millipore Sigma, and Cesium gluconate was purchased from HelloBio, all as dry powder.

Techniques: Flux Assay, Control, Selection, Western Blot, Expressing, Incubation