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Image Search Results
Journal: The Journal of General Virology
Article Title: Optimized cell systems for the investigation of hepatitis C virus E1E2 glycoproteins
doi: 10.1099/jgv.0.001512
Figure Lengend Snippet: ELISA analysis of antibody and CD81 binding to sE2 form susp-293T, susp-293T CD81KO and 293-F cells. sE2 was immobilized to Strep-TactinXT-coated microplates and incubated with a serial dilution of the indicated mAb (a) or CD81-LEL-hFc (b). Each point represents a single value from a lone experiment. Data were fitted using a sigmoidal curve in GraphPad Prism.
Article Snippet: The supernatant containing sE2 was harvested after 6 days and clarified using vacuum-filtration (0.2 µm) and sE2 was purified using
Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay, Incubation, Serial Dilution