Journal: Nature Communications

Article Title: Reversing insufficient photothermal therapy-induced tumor relapse and metastasis by regulating cancer-associated fibroblasts

doi: 10.1038/s41467-022-30306-7

Figure Lengend Snippet: a Schematic schedule for CD8 + T-cell tumor infiltration analysis in stroma-rich H22 tumor-bearing mice after intravenous injection of PBS, ICG, Cal/ICG, ICG@MPs, Cal@MPs, or Cal/ICG@MPs derived from H22 cells at the ICG dosage of 8 mg kg −1 and Cal dosage of 120 μg kg −1 , followed with or without 808 nm laser irradiation (1.5 W cm −2 , 10 min) at 2 h after the last injection. b – e Numbers of CD11c + CD80 + CD86 + cells ( b ), CD8 + CD69 + T cells ( c ), CD8 + IFN-γ + T cells ( d ), and CD8 + IL-2 + T cells ( e ) in tumor tissues of stroma-rich H22 tumor-bearing mice after treatment indicated in a . Data are presented as means ± s.d. ( n = 6 mice per group; one-way ANOVA followed by Tukey’s HSD post hoc test). f Immunofluorescence images of CD8 + T cells (labeling with Cy3-conjugated CD8 antibody, green) and CAFs (labeling with Cy5-conjugated α-SMA antibody, red) in tumor tissues of stroma-rich H22 tumor-bearing mice after intravenous injection of PBS, ICG, Cal/ICG, ICG@MPs or Cal/ICG@MPs at the ICG dosage of 8 mg kg −1 and Cal dosage of 120 μg kg −1 , followed with 808 nm laser irradiation indicated in a . The tumor margin was marked with a white dotted line. Images are representative of three biologically independent mice. Scale bar: 100 μm. g CD8 + T-cell numbers in tumor tissues of stroma-rich H22 tumor-bearing mice after treatment indicated in f analyzed by StrataQuest software. Data are presented as means ± s.d. ( n = 3 fields in total from two mice; one-way ANOVA followed by Tukey’s HSD post hoc test). h CD8 + T-cell numbers within 50 μm distance around CAFs in tumor tissues of stroma-rich H22 tumor-bearing mice after treatment indicated in f . Data are presented as means ± s.d. ( n = 3 fields in total from two mice; one-way ANOVA followed by Tukey’s HSD post hoc test). i CD8 + T-cell numbers from the margin to center areas in tumor tissues of stroma-rich H22 tumor-bearing mice after treatment indicated in f . Data are presented as means ± s.d. ( n = 6 fields in total from three mice; two-way ANOVA followed by Tukey’s multiple comparisons post test). Source data and exact P values for b , c , i are provided as a Source Data file.

Article Snippet: Immunofluorescence images of CD8 + T cells (labeling with Cy3-conjugated CD8 antibody, green) and CAFs (labeling with Cy5-conjugated α-SMA antibody, red) in tumor tissues of stroma-rich H22 tumor-bearing mice were acquired using Pannoramic Scanner (3DHISTECH, Hungary) and analyzed using CaseViewer (3DHISTECH, Hungary) and StrataQuest tissue analysis software (TissueGnostics, Asia Pacific limited, China) as described previously .

Techniques: Injection, Derivative Assay, Irradiation, Immunofluorescence, Labeling, Software

Journal: Cancers

Article Title: The Prognostic and Predictive Value of SOX2 + Cell Densities in Patients Treated for Colorectal Cancer

doi: 10.3390/cancers12051110

Figure Lengend Snippet: SOX2 (sex-determining region-Y homeobox-2) staining and quantification. Representative images of ( A ) SOX2 low and ( B ) high immunostaining and ( C ) digital analysis of the SOX2 high sample using StrataQuest software. Scale bars 100 μm.

Article Snippet: SOX2 expression was analyzed using StrataQuest v. 6 software (TissueGnostics, Vienna, Austria).

Techniques: Staining, Immunostaining, Software