Journal:

Article Title: Immobilization of Arsenite and Ferric Iron by Acidithiobacillus ferrooxidans and Its Relevance to Acid Mine Drainage

doi: 10.1128/AEM.69.10.6165-6173.2003

Figure Lengend Snippet: Arsenic removal by different A. ferrooxidans strains in Fe(II)-As(III) medium. Strains: type strain ATCC 23270 (triangles); ATCC 33020 (squares); ATCC 19859 (diamonds); and BRGM1 (circles). The concentrations of soluble As (open symbols) and As(III) (solid symbols) were determined.

Article Snippet: Different A. ferrooxidans strains (ATCC 19859, ATCC 33020, ATCC 23270, and BRGM1) were grown on Fe(II)-As(III) buffered medium, and the profiles of dissolved As(III) and As were followed throughout growth.

Techniques:

Journal: Biochimica et biophysica acta

Article Title: The bc(1) complex of the iron-grown acidophilic chemolithotrophic bacterium Acidithiobacillus ferrooxidans functions in the reverse but not in the forward direction. Is there a second bc(1) complex?

doi: 10.1016/s0005-2728(02)00251-7

Figure Lengend Snippet: Fig. 1. Thermodynamic profile of the redox centers of the A. ferrooxidans bc1 complex when the value of the external pH is 7.4 (A) or 3.5 (B) compared with profiles of typical bc1 (continuous boxes) and b6f complexes (dashed boxes) (C). Based on the data obtained on the c type cytochromes, it was not possible to decide which of the two redox species at 150 and 485 mV (pH 7.4) and at 240 and 560 mV (pH 3.5) belongs to cytochrome c1 and which to cytochrome c 46 kDa (figure adapted from Ref. [18]).

Article Snippet: A search for the sequence of the three redox subunits of the A. ferrooxidans bc1 complex (strain ATCC 19859) in the complete genome sequence of the A. ferrooxidans ATCC 23270 strain showed the existence of two different bc1 complexes in A. ferrooxidans.

Techniques:

Journal: Biochimica et biophysica acta

Article Title: The bc(1) complex of the iron-grown acidophilic chemolithotrophic bacterium Acidithiobacillus ferrooxidans functions in the reverse but not in the forward direction. Is there a second bc(1) complex?

doi: 10.1016/s0005-2728(02)00251-7

Figure Lengend Snippet: Fig. 2. Sequence alignments of cytochrome b (A) and Rieske protein (B) of bc1 complexes. The -P-E272-W-Y- sequence of cytochrome b (A), the two boxes -C129-X-H-L- and -C148-P-C-H-, which contain the [2Fe–2S] cluster ligands, and the -P165-A-P- sequence of the Rieske protein (B) are shaded in gray. Stars indicate some of the totally conserved residues like the four histidine (H82, H96, H183, H197) ligands of the two cytochrome b hemes (A) and C134 involved in the disulfide bridge with C150 in the Rieske protein (B). Arrows indicate amino acids discussed in the text. A. ferro1: A. ferrooxidans locus 1; A. ferro2: A. ferrooxidans locus 2; S. cerev.: S. cerevisiae; N. europ.: Nitrosomonas europea. The alignment was performed using the program CLUSTAL W.

Article Snippet: A search for the sequence of the three redox subunits of the A. ferrooxidans bc1 complex (strain ATCC 19859) in the complete genome sequence of the A. ferrooxidans ATCC 23270 strain showed the existence of two different bc1 complexes in A. ferrooxidans.

Techniques: Sequencing

Journal:

Article Title: ISAfe1, an ISL3 Family Insertion Sequence from Acidithiobacillus ferrooxidans ATCC 19859

doi: 10.1128/JB.183.14.4323-4329.2001

Figure Lengend Snippet: Southern blot analysis of several strains of A. ferrooxidans (lanes 1 to 12) and A. thiooxidans (lanes 13 and 14). Genomic DNA was cut with BamHI and hybridized with 32P-labeled probe derived from an internal SphI fragment of ISAfe1. Lanes: 1, ATTC 23270; 2, ATCC 13661 isolate A; 3, ATCC 13661 isolate B; 4, DSH1; 5, Duval; 6, DSM; 7, DSH2; 8, ATCC 14119; 9, ATCC 19859; 10, Torma; 11, Pyrite; 12, Penn Coal; 13, ATCC 8085; 14, ATCC 15494.

Article Snippet: E. coli was grown in Luria-Bertani (LB) medium ( 30 ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Genotype and/or source a Strains Acidithiobacillus ferrooxidans 19859 ATCC 23270 ATCC 14119 ATCC 13361 isolate A ATCC (clonal isolate A) 13361 isolate B ATCC (clonal isolate B) Torma H. Ehrlich Duval H. Ehrlich DSM H. Ehrlich Pyrite H. Ehrlich Penn Coal H. Ehrlich DSH1 This study DSH2 This study Thiobacillus thiooxidans 8085 ATCC 15494 ATCC Leptospirillum ferrooxidans G. Karavieko Escherichia coli NK7379 recA Δ( lac ) MiniF′Tc r (48) MC4100 [ ara Δ 139 Δ ( lacIPOZYA-argF )U169 rpsL thi ] TGlacIq supE hsd Δ 5 thi Δ( lac-proAB ) F tra Δ 36 proAB + lacZ ΔM15 Plasmids pTf85 Bam HI fragment carrying ISAfe1 cloned into pBR322 (this study) pACYC184 11 pACYC184-ISAfe1 Bam HI fragment carrying ISAfe1 from pTF58 cloned into pACYC184 (this study) Open in a separate window a ATCC, American Type Culture Collection.

Techniques: Southern Blot, Labeling, Derivative Assay

Journal:

Article Title: Construction and Characterization of a recA Mutant of Thiobacillus ferrooxidans by Marker Exchange Mutagenesis

doi:

Figure Lengend Snippet: Bacteria and plasmids used in this study

Article Snippet: The IncQ plasmid pJRD215 was stable, especially in the two collection strains ATCC 19859 and ATCC 33020, with more than 70% retention after 40 generations without antibiotic selection (Fig. A).

Techniques: Plasmid Preparation, Isolation, Clone Assay

Journal:

Article Title: Construction and Characterization of a recA Mutant of Thiobacillus ferrooxidans by Marker Exchange Mutagenesis

doi:

Figure Lengend Snippet: Stability analysis of pJRD215 (IncQ) (A), pUFR034 (IncW) (B), and pJB3Km1 (IncP) (C) in T. ferrooxidans ATCC 33020, ATCC 19859, BRGM1, and Tf-49 strains.

Article Snippet: The IncQ plasmid pJRD215 was stable, especially in the two collection strains ATCC 19859 and ATCC 33020, with more than 70% retention after 40 generations without antibiotic selection (Fig. A).

Techniques: