Journal: Stem Cell Research & Therapy

Article Title: Translational models of 3-D organoids and cancer stem cells in gastric cancer research

doi: 10.1186/s13287-021-02521-4

Figure Lengend Snippet: Effect of ROCK inhibitor in gastric organoid culture. The rock inhibitor supplied in culture medium maintained the growth and morphologies of the organoids well and helped to avoid the culture-damages caused by the culture-environments. ( a ) The cultivation in the absence of the ROCK inhibitor (left lane) caused the growth of the organoids slowly. ( b ) Addition of ROCK inhibitor (10 µM; right panel) resulted in the growth of the organoids 1.5–2.0-fold faster compared with the control organoids (left panel). Human adenocarcinoma of stomach organoid lines, HCM-BROD-0208-C16 cancer model primary adenocarcinoma of stomach was purchased from American Type Culture Collection (PDM-146™; ATCC, VA, USA) and cultured as its recommended protocol (bright view, scale bar = 100 μm). ( c ) The stomach organoids were generated by seeding in 96-well plates and grown 2 days ROCK inhibitor (n = 6): Th organoids were incubated with CellTiter-Glo®3D reagent (G9681, Promega, Madison, WL, USA) prior to culture medium addition, and results were acquired to assay (** P < 0.01).

Article Snippet: Human adenocarcinoma of stomach organoid lines, HCM-BROD-0208-C16 cancer model primary adenocarcinoma of stomach was purchased from American Type Culture Collection (PDM-146TM; ATCC, USA) and cultured as its recommended protocol [ ] (bright view images, scale bar = 100 μm). ( c ) The stomach organoids were generated by seeding in 96-well plates and grown 2 days GSK inhibitor (n = 6): Th organoids were incubated with CellTiter-Glo®3D reagent (G9681, Promega, Madison, WL, USA) prior to culture medium addition, and results were acquired to assay (*** P < 0.001) Fig. 6 Effect of niche factors in gastric organoid culture.

Techniques: Control, Cell Culture, Generated, Incubation

Journal: Stem Cell Research & Therapy

Article Title: Translational models of 3-D organoids and cancer stem cells in gastric cancer research

doi: 10.1186/s13287-021-02521-4

Figure Lengend Snippet: Effect of niche factors in gastric organoid culture. The concentration of R-SPONDIN 1 in the gastric cancer organoids culture was 10%, and the concentration of Wnt3a in the cancer organoids was 20% (panel a ) and 10% (panel b ). Human adenocarcinoma of stomach organoid lines, HCM-BROD-0208-C16 cancer model primary adenocarcinoma of stomach was purchased from American Type Culture Collection (PDM-146™; ATCC, USA) and cultured as its recommended protocol (bright view images scale bar = 50 μm). ( c ) The stomach organoids were generated by seeding in 96-well plates and grown 2 days at different percentages of Wnt (n = 6). Th organoids were incubated with CellTiter-Glo®3D reagent (G9681, Promega, Madison, WL,USA) prior to culture medium addition, and results were acquired to assay (*** P < 0.001),

Article Snippet: Human adenocarcinoma of stomach organoid lines, HCM-BROD-0208-C16 cancer model primary adenocarcinoma of stomach was purchased from American Type Culture Collection (PDM-146TM; ATCC, USA) and cultured as its recommended protocol [ ] (bright view images, scale bar = 100 μm). ( c ) The stomach organoids were generated by seeding in 96-well plates and grown 2 days GSK inhibitor (n = 6): Th organoids were incubated with CellTiter-Glo®3D reagent (G9681, Promega, Madison, WL, USA) prior to culture medium addition, and results were acquired to assay (*** P < 0.001) Fig. 6 Effect of niche factors in gastric organoid culture.

Techniques: Concentration Assay, Cell Culture, Generated, Incubation

Journal: Genome biology

Article Title: Highly recurrent CBS epimutations in gastric cancer CpG island methylator phenotypes and inflammation.

doi: 10.1186/s13059-021-02375-2

Figure Lengend Snippet: Fig. 2 CBS epimutations associate with CIMP in primary GCs. a Overlap of genes that are promoter hypermethylated and downregulated at the RNA level in the discovery GC cell line panel, TCGA-stomach adenocarcinoma [STAD] and Singapore [SG] cohorts (β-value difference ≥0.3 and q-value < 0.05) [left panel]. Average promoter methylation β-values and gene expression of CBS gene in STAD according to CIMP subtypes (*P < 0.001, two-tailed Wilcoxon rank sum test, each CIMP group vs. non-CIMP group) [right panel]. b Immunohistochemistry of CBS in a normal human stomach with black arrow indicating cytoplasmic staining in epithelial cells. Control sections were not treated with the primary antibody. c Summary of CBS staining in 66 cases of matched normal and gastric adenocarcinomas (*P < 0.05, two- tailed Fisher’s exact test) [left panel] and an example of a matched normal vs. tumor case with a negative score [right panel]. d Summary of somatic and germline genetic alterations at CBS in STAD. PALP, pyridoxal- phosphate dependent enzyme domain; CBS, cystathionine beta-synthase domain; aa, amino acid

Article Snippet: Normal human stomach antrum and fundus tissue slides were purchased from Novus Biologicals (NBP2-30203, NBP230204).

Techniques: Methylation, Gene Expression, Two Tailed Test, Immunohistochemistry, Staining, Control