stimulation Search Results


standalone aversive electric shock 731 stimulator  (AutoMate Scientific Inc)
97
AutoMate Scientific Inc standalone aversive electric shock 731 stimulator
Standalone Aversive Electric Shock 731 Stimulator, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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granulocyte macrophage colony  (Cell Signaling Technology Inc)
92
Cell Signaling Technology Inc granulocyte macrophage colony
Granulocyte Macrophage Colony, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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electric stimulator  (Digitimer North America LLC)
96
Digitimer North America LLC electric stimulator
Electric Stimulator, supplied by Digitimer North America LLC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ds2a  (Digitimer North America LLC)
96
Digitimer North America LLC ds2a
Ds2a, supplied by Digitimer North America LLC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phosphorylated erk  (Proteintech)
96
Proteintech phosphorylated erk
Phosphorylated Erk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eia elisa kit  (Elabscience Biotechnology)
95
Elabscience Biotechnology eia elisa kit
Luteolysis is impaired in 6-month-old F2 ovaries. a FSH and LH secretion in serum samples from 6-month-old ovaries measured by <t>ELISA</t> ( n = 11 control (C) and n = 5 F2 ovaries). b PGF 2α and PGE 2 production in 6-month-old ovaries measured by ELISA ( n = 3 control (C) and n = 5 F2 ovaries). c Relative expression levels of the steroidogenic genes StAR, Cyp11a1, Hsd3β, Lhcgr and 20αHsd in 6-month-old F2 ovaries normalised to 18 S ; ** P < 0.01, **** P < 0.001. d Progesterone secretion was measured by LC/MS in 6-month-old control ( n = 6) and F2 ( n = 5) ovaries; ns not significant. e Proliferation in 2-month-old F1 and F2 and in 6-month-old F2 ovaries was assessed by immunofluorescence using anti-PCNA (green) antibody and Hoechst (HST, blue); CL = corpus luteum; white arrows highlight proliferating CLs and arrowheads indicated stromal cells. f , g Apoptotic cells in 2-month-old F1 and F2 and in 6-month-old F2 ovaries were visualised by TUNEL assay (green) and nuclear staining with Hoechst (HST, blue) ( f ) and apoptotic corpora lutea (CL) were counted ( g ); the data are presented as the percentage of apoptotic CLs. * P < 0.05 ( g ). White and blue arrows indicate apoptotic and non-apoptotic CLs, respectively, and arrowheads indicate growing follicles ( f ). Scale bars = 300 μm. h – j The AKT-PDK1 pathway is activated in 6-month-old F2 ovaries compared with controls. Relative expression levels of Pi3k , Pdk1 , Pten and Mtor normalised to 18 S , in control and F2 ovaries; ** P < 0.01, *** P < 0.005 ( h ). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin ( i ) show that the AKT pathway is activated in F2 ovaries ( n = 3), as indicated by the increased P-AKT/AKT ratio compared with control ( n = 4); *** P < 0.005 ( j )
Eia Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa kits e el m1153 tsh  (Elabscience Biotechnology)
94
Elabscience Biotechnology elisa kits e el m1153 tsh
Luteolysis is impaired in 6-month-old F2 ovaries. a FSH and LH secretion in serum samples from 6-month-old ovaries measured by <t>ELISA</t> ( n = 11 control (C) and n = 5 F2 ovaries). b PGF 2α and PGE 2 production in 6-month-old ovaries measured by ELISA ( n = 3 control (C) and n = 5 F2 ovaries). c Relative expression levels of the steroidogenic genes StAR, Cyp11a1, Hsd3β, Lhcgr and 20αHsd in 6-month-old F2 ovaries normalised to 18 S ; ** P < 0.01, **** P < 0.001. d Progesterone secretion was measured by LC/MS in 6-month-old control ( n = 6) and F2 ( n = 5) ovaries; ns not significant. e Proliferation in 2-month-old F1 and F2 and in 6-month-old F2 ovaries was assessed by immunofluorescence using anti-PCNA (green) antibody and Hoechst (HST, blue); CL = corpus luteum; white arrows highlight proliferating CLs and arrowheads indicated stromal cells. f , g Apoptotic cells in 2-month-old F1 and F2 and in 6-month-old F2 ovaries were visualised by TUNEL assay (green) and nuclear staining with Hoechst (HST, blue) ( f ) and apoptotic corpora lutea (CL) were counted ( g ); the data are presented as the percentage of apoptotic CLs. * P < 0.05 ( g ). White and blue arrows indicate apoptotic and non-apoptotic CLs, respectively, and arrowheads indicate growing follicles ( f ). Scale bars = 300 μm. h – j The AKT-PDK1 pathway is activated in 6-month-old F2 ovaries compared with controls. Relative expression levels of Pi3k , Pdk1 , Pten and Mtor normalised to 18 S , in control and F2 ovaries; ** P < 0.01, *** P < 0.005 ( h ). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin ( i ) show that the AKT pathway is activated in F2 ovaries ( n = 3), as indicated by the increased P-AKT/AKT ratio compared with control ( n = 4); *** P < 0.005 ( j )
Elisa Kits E El M1153 Tsh, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mladdf30 stimulating bar electrode  (ADInstruments)
94
ADInstruments mladdf30 stimulating bar electrode
Luteolysis is impaired in 6-month-old F2 ovaries. a FSH and LH secretion in serum samples from 6-month-old ovaries measured by <t>ELISA</t> ( n = 11 control (C) and n = 5 F2 ovaries). b PGF 2α and PGE 2 production in 6-month-old ovaries measured by ELISA ( n = 3 control (C) and n = 5 F2 ovaries). c Relative expression levels of the steroidogenic genes StAR, Cyp11a1, Hsd3β, Lhcgr and 20αHsd in 6-month-old F2 ovaries normalised to 18 S ; ** P < 0.01, **** P < 0.001. d Progesterone secretion was measured by LC/MS in 6-month-old control ( n = 6) and F2 ( n = 5) ovaries; ns not significant. e Proliferation in 2-month-old F1 and F2 and in 6-month-old F2 ovaries was assessed by immunofluorescence using anti-PCNA (green) antibody and Hoechst (HST, blue); CL = corpus luteum; white arrows highlight proliferating CLs and arrowheads indicated stromal cells. f , g Apoptotic cells in 2-month-old F1 and F2 and in 6-month-old F2 ovaries were visualised by TUNEL assay (green) and nuclear staining with Hoechst (HST, blue) ( f ) and apoptotic corpora lutea (CL) were counted ( g ); the data are presented as the percentage of apoptotic CLs. * P < 0.05 ( g ). White and blue arrows indicate apoptotic and non-apoptotic CLs, respectively, and arrowheads indicate growing follicles ( f ). Scale bars = 300 μm. h – j The AKT-PDK1 pathway is activated in 6-month-old F2 ovaries compared with controls. Relative expression levels of Pi3k , Pdk1 , Pten and Mtor normalised to 18 S , in control and F2 ovaries; ** P < 0.01, *** P < 0.005 ( h ). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin ( i ) show that the AKT pathway is activated in F2 ovaries ( n = 3), as indicated by the increased P-AKT/AKT ratio compared with control ( n = 4); *** P < 0.005 ( j )
Mladdf30 Stimulating Bar Electrode, supplied by ADInstruments, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stimulator fe155 stimulator hc adinstrument oxford uk  (ADInstruments)
95
ADInstruments stimulator fe155 stimulator hc adinstrument oxford uk
Luteolysis is impaired in 6-month-old F2 ovaries. a FSH and LH secretion in serum samples from 6-month-old ovaries measured by <t>ELISA</t> ( n = 11 control (C) and n = 5 F2 ovaries). b PGF 2α and PGE 2 production in 6-month-old ovaries measured by ELISA ( n = 3 control (C) and n = 5 F2 ovaries). c Relative expression levels of the steroidogenic genes StAR, Cyp11a1, Hsd3β, Lhcgr and 20αHsd in 6-month-old F2 ovaries normalised to 18 S ; ** P < 0.01, **** P < 0.001. d Progesterone secretion was measured by LC/MS in 6-month-old control ( n = 6) and F2 ( n = 5) ovaries; ns not significant. e Proliferation in 2-month-old F1 and F2 and in 6-month-old F2 ovaries was assessed by immunofluorescence using anti-PCNA (green) antibody and Hoechst (HST, blue); CL = corpus luteum; white arrows highlight proliferating CLs and arrowheads indicated stromal cells. f , g Apoptotic cells in 2-month-old F1 and F2 and in 6-month-old F2 ovaries were visualised by TUNEL assay (green) and nuclear staining with Hoechst (HST, blue) ( f ) and apoptotic corpora lutea (CL) were counted ( g ); the data are presented as the percentage of apoptotic CLs. * P < 0.05 ( g ). White and blue arrows indicate apoptotic and non-apoptotic CLs, respectively, and arrowheads indicate growing follicles ( f ). Scale bars = 300 μm. h – j The AKT-PDK1 pathway is activated in 6-month-old F2 ovaries compared with controls. Relative expression levels of Pi3k , Pdk1 , Pten and Mtor normalised to 18 S , in control and F2 ovaries; ** P < 0.01, *** P < 0.005 ( h ). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin ( i ) show that the AKT pathway is activated in F2 ovaries ( n = 3), as indicated by the increased P-AKT/AKT ratio compared with control ( n = 4); *** P < 0.005 ( j )
Stimulator Fe155 Stimulator Hc Adinstrument Oxford Uk, supplied by ADInstruments, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell lysate mixture  (Proteintech)
96
Proteintech cell lysate mixture
Luteolysis is impaired in 6-month-old F2 ovaries. a FSH and LH secretion in serum samples from 6-month-old ovaries measured by <t>ELISA</t> ( n = 11 control (C) and n = 5 F2 ovaries). b PGF 2α and PGE 2 production in 6-month-old ovaries measured by ELISA ( n = 3 control (C) and n = 5 F2 ovaries). c Relative expression levels of the steroidogenic genes StAR, Cyp11a1, Hsd3β, Lhcgr and 20αHsd in 6-month-old F2 ovaries normalised to 18 S ; ** P < 0.01, **** P < 0.001. d Progesterone secretion was measured by LC/MS in 6-month-old control ( n = 6) and F2 ( n = 5) ovaries; ns not significant. e Proliferation in 2-month-old F1 and F2 and in 6-month-old F2 ovaries was assessed by immunofluorescence using anti-PCNA (green) antibody and Hoechst (HST, blue); CL = corpus luteum; white arrows highlight proliferating CLs and arrowheads indicated stromal cells. f , g Apoptotic cells in 2-month-old F1 and F2 and in 6-month-old F2 ovaries were visualised by TUNEL assay (green) and nuclear staining with Hoechst (HST, blue) ( f ) and apoptotic corpora lutea (CL) were counted ( g ); the data are presented as the percentage of apoptotic CLs. * P < 0.05 ( g ). White and blue arrows indicate apoptotic and non-apoptotic CLs, respectively, and arrowheads indicate growing follicles ( f ). Scale bars = 300 μm. h – j The AKT-PDK1 pathway is activated in 6-month-old F2 ovaries compared with controls. Relative expression levels of Pi3k , Pdk1 , Pten and Mtor normalised to 18 S , in control and F2 ovaries; ** P < 0.01, *** P < 0.005 ( h ). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin ( i ) show that the AKT pathway is activated in F2 ovaries ( n = 3), as indicated by the increased P-AKT/AKT ratio compared with control ( n = 4); *** P < 0.005 ( j )
Cell Lysate Mixture, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cxcl12  (Bio X Cell)
93
Bio X Cell anti cxcl12
Luteolysis is impaired in 6-month-old F2 ovaries. a FSH and LH secretion in serum samples from 6-month-old ovaries measured by <t>ELISA</t> ( n = 11 control (C) and n = 5 F2 ovaries). b PGF 2α and PGE 2 production in 6-month-old ovaries measured by ELISA ( n = 3 control (C) and n = 5 F2 ovaries). c Relative expression levels of the steroidogenic genes StAR, Cyp11a1, Hsd3β, Lhcgr and 20αHsd in 6-month-old F2 ovaries normalised to 18 S ; ** P < 0.01, **** P < 0.001. d Progesterone secretion was measured by LC/MS in 6-month-old control ( n = 6) and F2 ( n = 5) ovaries; ns not significant. e Proliferation in 2-month-old F1 and F2 and in 6-month-old F2 ovaries was assessed by immunofluorescence using anti-PCNA (green) antibody and Hoechst (HST, blue); CL = corpus luteum; white arrows highlight proliferating CLs and arrowheads indicated stromal cells. f , g Apoptotic cells in 2-month-old F1 and F2 and in 6-month-old F2 ovaries were visualised by TUNEL assay (green) and nuclear staining with Hoechst (HST, blue) ( f ) and apoptotic corpora lutea (CL) were counted ( g ); the data are presented as the percentage of apoptotic CLs. * P < 0.05 ( g ). White and blue arrows indicate apoptotic and non-apoptotic CLs, respectively, and arrowheads indicate growing follicles ( f ). Scale bars = 300 μm. h – j The AKT-PDK1 pathway is activated in 6-month-old F2 ovaries compared with controls. Relative expression levels of Pi3k , Pdk1 , Pten and Mtor normalised to 18 S , in control and F2 ovaries; ** P < 0.01, *** P < 0.005 ( h ). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin ( i ) show that the AKT pathway is activated in F2 ovaries ( n = 3), as indicated by the increased P-AKT/AKT ratio compared with control ( n = 4); *** P < 0.005 ( j )
Anti Cxcl12, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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exogenous stimulations  (ADInstruments)
93
ADInstruments exogenous stimulations
Luteolysis is impaired in 6-month-old F2 ovaries. a FSH and LH secretion in serum samples from 6-month-old ovaries measured by <t>ELISA</t> ( n = 11 control (C) and n = 5 F2 ovaries). b PGF 2α and PGE 2 production in 6-month-old ovaries measured by ELISA ( n = 3 control (C) and n = 5 F2 ovaries). c Relative expression levels of the steroidogenic genes StAR, Cyp11a1, Hsd3β, Lhcgr and 20αHsd in 6-month-old F2 ovaries normalised to 18 S ; ** P < 0.01, **** P < 0.001. d Progesterone secretion was measured by LC/MS in 6-month-old control ( n = 6) and F2 ( n = 5) ovaries; ns not significant. e Proliferation in 2-month-old F1 and F2 and in 6-month-old F2 ovaries was assessed by immunofluorescence using anti-PCNA (green) antibody and Hoechst (HST, blue); CL = corpus luteum; white arrows highlight proliferating CLs and arrowheads indicated stromal cells. f , g Apoptotic cells in 2-month-old F1 and F2 and in 6-month-old F2 ovaries were visualised by TUNEL assay (green) and nuclear staining with Hoechst (HST, blue) ( f ) and apoptotic corpora lutea (CL) were counted ( g ); the data are presented as the percentage of apoptotic CLs. * P < 0.05 ( g ). White and blue arrows indicate apoptotic and non-apoptotic CLs, respectively, and arrowheads indicate growing follicles ( f ). Scale bars = 300 μm. h – j The AKT-PDK1 pathway is activated in 6-month-old F2 ovaries compared with controls. Relative expression levels of Pi3k , Pdk1 , Pten and Mtor normalised to 18 S , in control and F2 ovaries; ** P < 0.01, *** P < 0.005 ( h ). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin ( i ) show that the AKT pathway is activated in F2 ovaries ( n = 3), as indicated by the increased P-AKT/AKT ratio compared with control ( n = 4); *** P < 0.005 ( j )
Exogenous Stimulations, supplied by ADInstruments, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Luteolysis is impaired in 6-month-old F2 ovaries. a FSH and LH secretion in serum samples from 6-month-old ovaries measured by ELISA ( n = 11 control (C) and n = 5 F2 ovaries). b PGF 2α and PGE 2 production in 6-month-old ovaries measured by ELISA ( n = 3 control (C) and n = 5 F2 ovaries). c Relative expression levels of the steroidogenic genes StAR, Cyp11a1, Hsd3β, Lhcgr and 20αHsd in 6-month-old F2 ovaries normalised to 18 S ; ** P < 0.01, **** P < 0.001. d Progesterone secretion was measured by LC/MS in 6-month-old control ( n = 6) and F2 ( n = 5) ovaries; ns not significant. e Proliferation in 2-month-old F1 and F2 and in 6-month-old F2 ovaries was assessed by immunofluorescence using anti-PCNA (green) antibody and Hoechst (HST, blue); CL = corpus luteum; white arrows highlight proliferating CLs and arrowheads indicated stromal cells. f , g Apoptotic cells in 2-month-old F1 and F2 and in 6-month-old F2 ovaries were visualised by TUNEL assay (green) and nuclear staining with Hoechst (HST, blue) ( f ) and apoptotic corpora lutea (CL) were counted ( g ); the data are presented as the percentage of apoptotic CLs. * P < 0.05 ( g ). White and blue arrows indicate apoptotic and non-apoptotic CLs, respectively, and arrowheads indicate growing follicles ( f ). Scale bars = 300 μm. h – j The AKT-PDK1 pathway is activated in 6-month-old F2 ovaries compared with controls. Relative expression levels of Pi3k , Pdk1 , Pten and Mtor normalised to 18 S , in control and F2 ovaries; ** P < 0.01, *** P < 0.005 ( h ). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin ( i ) show that the AKT pathway is activated in F2 ovaries ( n = 3), as indicated by the increased P-AKT/AKT ratio compared with control ( n = 4); *** P < 0.005 ( j )

Journal: Communications Biology

Article Title: In utero exposure to acetaminophen and ibuprofen leads to intergenerational accelerated reproductive aging in female mice

doi: 10.1038/s42003-019-0552-x

Figure Lengend Snippet: Luteolysis is impaired in 6-month-old F2 ovaries. a FSH and LH secretion in serum samples from 6-month-old ovaries measured by ELISA ( n = 11 control (C) and n = 5 F2 ovaries). b PGF 2α and PGE 2 production in 6-month-old ovaries measured by ELISA ( n = 3 control (C) and n = 5 F2 ovaries). c Relative expression levels of the steroidogenic genes StAR, Cyp11a1, Hsd3β, Lhcgr and 20αHsd in 6-month-old F2 ovaries normalised to 18 S ; ** P < 0.01, **** P < 0.001. d Progesterone secretion was measured by LC/MS in 6-month-old control ( n = 6) and F2 ( n = 5) ovaries; ns not significant. e Proliferation in 2-month-old F1 and F2 and in 6-month-old F2 ovaries was assessed by immunofluorescence using anti-PCNA (green) antibody and Hoechst (HST, blue); CL = corpus luteum; white arrows highlight proliferating CLs and arrowheads indicated stromal cells. f , g Apoptotic cells in 2-month-old F1 and F2 and in 6-month-old F2 ovaries were visualised by TUNEL assay (green) and nuclear staining with Hoechst (HST, blue) ( f ) and apoptotic corpora lutea (CL) were counted ( g ); the data are presented as the percentage of apoptotic CLs. * P < 0.05 ( g ). White and blue arrows indicate apoptotic and non-apoptotic CLs, respectively, and arrowheads indicate growing follicles ( f ). Scale bars = 300 μm. h – j The AKT-PDK1 pathway is activated in 6-month-old F2 ovaries compared with controls. Relative expression levels of Pi3k , Pdk1 , Pten and Mtor normalised to 18 S , in control and F2 ovaries; ** P < 0.01, *** P < 0.005 ( h ). Representative immunoblots of total AKT, phosphorylated AKT (P-AKT) and tubulin ( i ) show that the AKT pathway is activated in F2 ovaries ( n = 3), as indicated by the increased P-AKT/AKT ratio compared with control ( n = 4); *** P < 0.005 ( j )

Article Snippet: The concentration of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in serum was measured with specific EIA ELISA kit (ElabScience Biotech, E-EL-M0511 and E-EL-M0057 respectively, CliniSciences France).

Techniques: Enzyme-linked Immunosorbent Assay, Control, Expressing, Liquid Chromatography with Mass Spectroscopy, Immunofluorescence, TUNEL Assay, Staining, Western Blot