Journal: PLoS ONE

Article Title: Steviol Reduces MDCK Cyst Formation and Growth by Inhibiting CFTR Channel Activity and Promoting Proteasome-Mediated CFTR Degradation

doi: 10.1371/journal.pone.0058871

Figure Lengend Snippet: ( A ) Inhibitory effect of steviol and its derivatives on MDCK cyst formation. MDCK cysts were incubated with 100 µM of steviol and its derivatives in media containing forskolin (10 µM) after cell seeding on day 0 onward. The graph represents percent of cyst colonies at day 6 after MDCK cell seeding in the absence (control) and presence of all compounds (mean±SE; n = 4 wells/condition; **P <0.01 compared with control). ( B ) Inhibitory effect of steviol and its derivatives on MDCK cyst growth. The graph shows the outer cyst diameter at day 12 (mean±SE; n = 32−77 cysts; **P <0.01 compared with control). ( C ) Dose-response of effect of steviol on MDCK cyst growth. After cell seeding in 3D collagen gel for 6 days, media containing forskolin and steviol at doses of 50, 100, and 200 µM were added to the MDCK cells from day 6 onward. Results were shown as mean value of cyst diameter at days 6, 8, 10, and 12 ( n = 43−77 cysts; **P <0.01 compared with control). ( D ) Representative light micrographs show MDCK cyst growth in 3D collagen gel after seeding of MDCK cells for 6 days. Three independent experiments were performed. Forskolin (10 µM) without ( 2D, top ) or with steviol (100 µM) ( 2D, middle, bottom ) was added to the culture medium at day 6. To test for reversibility, steviol was removed at day 9 ( 2D, bottom ). Scale bar = 100 µm; magnification = ×10.

Article Snippet: Prolonged treatment (24 h) with steviol (100 microM) also strongly inhibited forskolin-stimulated apical chloride current, in part by reducing CFTR protein expression in MDCK cells.

Techniques: Incubation, Control

Journal: PLoS ONE

Article Title: Steviol Reduces MDCK Cyst Formation and Growth by Inhibiting CFTR Channel Activity and Promoting Proteasome-Mediated CFTR Degradation

doi: 10.1371/journal.pone.0058871

Figure Lengend Snippet: Under permeabilization conditions, MDCK cell monolayers were mounted in hemichambers filled with a chloride gradient buffer. ( A ) Representative currents of acute effect. Steviol at all doses was added to both apical and basolateral hemichambers after stimulation of the chloride current by forskolin (post-treatment) and the currents were recorded at 50, 100, and 200 µM of steviol. At the end of experiment, 50 µM of GlyH-101 was added. ( B ) Summary of the data for the acute effect of steviol on apical chloride current in basolaterally permeabilized MDCK cell monolayers. (4 separate experiments, mean±SE; n = 4; **P <0.01 compared with controls)

Article Snippet: Prolonged treatment (24 h) with steviol (100 microM) also strongly inhibited forskolin-stimulated apical chloride current, in part by reducing CFTR protein expression in MDCK cells.

Techniques:

Journal: PLoS ONE

Article Title: Steviol Reduces MDCK Cyst Formation and Growth by Inhibiting CFTR Channel Activity and Promoting Proteasome-Mediated CFTR Degradation

doi: 10.1371/journal.pone.0058871

Figure Lengend Snippet: MDCK cell monolayer treated with steviol at the indicated concentrations or vehicle for 24 h were mounted in hemichambers filled with Krebs' buffer solution. After stimulation of current by forskolin, steviol inhibition was seen to have inhibited I sc in a dose-dependent fashion. Representative tracings ( A ) and the summary of the data of 3–10 separate experiments ( B ) are shown (mean±SE; n = 3−10; **P <0.01 compared with controls). ( C ) Representative time-response tracings of short-circuit current measurements. Monolayers were pretreated with 100 µM of steviol for 5 minutes and 2, 6, 12, and 24 h. ( D ) Summary of the data from time-response experiments. (mean±SE; n = 7−16 separate experiments; **P <0.01 compared with controls)

Article Snippet: Prolonged treatment (24 h) with steviol (100 microM) also strongly inhibited forskolin-stimulated apical chloride current, in part by reducing CFTR protein expression in MDCK cells.

Techniques: Inhibition

Journal: PLoS ONE

Article Title: Steviol Reduces MDCK Cyst Formation and Growth by Inhibiting CFTR Channel Activity and Promoting Proteasome-Mediated CFTR Degradation

doi: 10.1371/journal.pone.0058871

Figure Lengend Snippet: After permeabilization of the basolateral membrane with amphotericin B, MDCK cell monolayers were mounted in hemichambers filled with a chloride gradient buffer. 20 µM of forskolin was added to stimulate apical chloride current. ( A ) Representative tracings of chronic effect (pretreatment with steviol for 5 minutes, 2, 6, 12, and 24 h) were shown. At the end of the experiment, 50 µM of Gly-H101 was added. ( B ) Summary of the data for the chronic effect of steviol on apical chloride current in permeabilized MDCK cell monolayers (mean±SE; n = 5−11 separate experiments; **P <0.01 compared with controls)

Article Snippet: Prolonged treatment (24 h) with steviol (100 microM) also strongly inhibited forskolin-stimulated apical chloride current, in part by reducing CFTR protein expression in MDCK cells.

Techniques: Membrane

Journal: Foods

Article Title: Effect of Sugar Substitution with Steviol Glycosides on Sensory Quality and Physicochemical Composition of Low-Sugar Apple Preserves

doi: 10.3390/foods9030293

Figure Lengend Snippet: The composition of evaluated low-sugar (L) apple preserves in 3 stages of experiments for 100 g of apple pulp.

Article Snippet: For sugar substitution, high purity (95.1%, HPLC) powdered steviol glycosides (Stevia Natura SAS firm, Riom, Auvergne, France) of steviol equivalent—363 (mg/g) was used.

Techniques: Variant Assay

Journal: Molecules

Article Title: Structures of Some Novel α-Glucosyl Diterpene Glycosides from the Glycosylation of Steviol Glycosides

doi: 10.3390/molecules191220280

Figure Lengend Snippet: Structures of the new steviol glycosides 1 – 4 .

Article Snippet: Purification of a glucosylated steviol glycoside mixture obtained from PureCircle (Enstek, Malaysia) resulted in the isolation of four new additional minor diterpene glycosides – .

Techniques:

Journal: Molecules

Article Title: Structures of Some Novel α-Glucosyl Diterpene Glycosides from the Glycosylation of Steviol Glycosides

doi: 10.3390/molecules191220280

Figure Lengend Snippet: RP-HPLC methods for the isolation and purification of steviol glycosides 1 – 4 .

Article Snippet: Purification of a glucosylated steviol glycoside mixture obtained from PureCircle (Enstek, Malaysia) resulted in the isolation of four new additional minor diterpene glycosides – .

Techniques: Isolation, Purification