Journal: bioRxiv

Article Title: Profiling of microglia nodules in multiple sclerosis reveals propensity for lesion formation

doi: 10.1101/2023.06.11.544204

Figure Lengend Snippet: STED microscopy of (a) a microglia nodule in MS containing lysosomal (Lamp1) oxidized phospholipids (E06) and (b) a microglia nodule in stroke that does not show oxidized phospholipids (E06) in the lysosomes (Lamp1). (c) Microglia nodules in MS have more often phagocytosed oxidized phospholipids compared to microglia nodules in stroke. Bar plot shows mean value ± standard deviation. Significance was tested with a quasibinomial generalized linear model, p value <0.001 is indicated with ***. (d) In MS NAWM in the optic nerve, partially demyelinated axons (PLP - ) encapsulated by microglial nodules were found.

Article Snippet: The SMI312, PLP, and HLA triple staining, the LAMP1, E06, and HLA triple staining and the Tomm20 and HLA double staining were visualized at 63x using stimulated emission depletion (STED) microscopy (STEDYCON; Abberior Instruments, Göttingen, Germany) for z-stacks.

Techniques: Microscopy, Standard Deviation

Journal: bioRxiv

Article Title: Profiling of microglia nodules in multiple sclerosis reveals propensity for lesion formation

doi: 10.1101/2023.06.11.544204

Figure Lengend Snippet: STED microscopy of (a) an HLA + microglia nodule in MS with a fu ed and tubular TOM20 mitochondrial network and (b) an HLA + microglia nodule in stroke with a fragmented TOM20 mitochondrial network. (c) Microglia nodules in MS more often showed a tubular and fused mitochondrial network compared to microglia nodules in stroke. Bar plot showing mean percentage per type of mitochondrial network in MS and in stroke. Significance was tested with a quasibinomial linear model, p value <0.01 is indicated with **.

Article Snippet: The SMI312, PLP, and HLA triple staining, the LAMP1, E06, and HLA triple staining and the Tomm20 and HLA double staining were visualized at 63x using stimulated emission depletion (STED) microscopy (STEDYCON; Abberior Instruments, Göttingen, Germany) for z-stacks.

Techniques: Microscopy

Journal: International Journal of Molecular Sciences

Article Title: Differential Mitochondrial, Oxidative Stress and Inflammatory Responses to SARS-CoV-2 Spike Protein Receptor Binding Domain in Human Lung Microvascular, Coronary Artery Endothelial and Bronchial Epithelial Cells

doi: 10.3390/ijms25063188

Figure Lengend Snippet: The effect of SCoV2-RBD on the mitochondrial network morphology of HLMVECs. ( a ) Representative images of the mitochondrial network of untreated (Control) and SCoV2-RBD-treated HLMVECs (Spike). Live Mito Orange and images stained the cells were taken by an Olympus IX83 confocal microscope equipped with a STEDYCON STED nanoscope. The scale bar was 5 μm for the mitochondrial network of entire cells and 1 μm for the ROIs. ( b ) The quantitative results of the mitochondrial network parameters calculated by the MiNa plugin in the Fiji software. Results were normalised to untreated cells (Control) and represented as fold changes. C—Control, S—Spike, which means SCoV2-RBD treatment. * p < 0.05, ** p < 0.01; two-tailed Student’s t -test.

Article Snippet: Cristae were observed by an Olympus IX83 microscope (Olympus Corporation, Tokyo, Japan) with a STEDYCON STED nanoscope (Abberior, Goettingen, Germany) and UPLXAPO100xO NA1.45 objective.

Techniques: Control, Staining, Microscopy, Software, Two Tailed Test

Journal: International Journal of Molecular Sciences

Article Title: Differential Mitochondrial, Oxidative Stress and Inflammatory Responses to SARS-CoV-2 Spike Protein Receptor Binding Domain in Human Lung Microvascular, Coronary Artery Endothelial and Bronchial Epithelial Cells

doi: 10.3390/ijms25063188

Figure Lengend Snippet: Mitochondrial network analysis steps and explanation of parameters. A representative STED microscopy image of a live untreated (control) human lung microvascular endothelial cell (HLMVEC) after Live Mito Orange staining. The scale bar was 2 μm. ROI—region of interest.

Article Snippet: Cristae were observed by an Olympus IX83 microscope (Olympus Corporation, Tokyo, Japan) with a STEDYCON STED nanoscope (Abberior, Goettingen, Germany) and UPLXAPO100xO NA1.45 objective.

Techniques: Microscopy, Control, Staining