statistical analyses version 12.2.43f Search Results


plox gfp irestk  (Addgene inc)
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maize inbred varieties ph1v69  (ATCC)
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drebrin a  (Cell Signaling Technology Inc)
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bluo gal qiafilter plasmid midi kit qiagen dmrie c 10459014  (Qiagen)
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qiaquick pcr purification kit qiagen  (Qiagen)
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tgf b1  (Addgene inc)
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anti lcat polyclonal antibody  (Proteintech)
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Proteintech anti lcat polyclonal antibody
Primers used for real-time PCR analysis
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midiprep scale dna purification  (Qiagen)
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Qiagen midiprep scale dna purification
Primers used for real-time PCR analysis
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qiafilter plasmid midi kit  (Qiagen)
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Primers used for real-time PCR analysis
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qiafilter plasmid maxi kit  (Qiagen)
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Qiagen qiafilter plasmid maxi kit
Primers used for real-time PCR analysis
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methylorubrum zatmanii comb. nov  (NCIMB Ltd)
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NCIMB Ltd methylorubrum zatmanii comb. nov
Primers used for real-time PCR analysis
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Image Search Results


Primers used for real-time PCR analysis

Journal: Lipids in Health and Disease

Article Title: Chronic unpredictable mild stress combined with a high-fat diets aggravates atherosclerosis in rats

doi: 10.1186/1476-511X-13-77

Figure Lengend Snippet: Primers used for real-time PCR analysis

Article Snippet: LCAT, CYP7A1, LXR α, SR-BI, ABCG5 and ABCG8 proteins were detected using an anti-LCAT polyclonal antibody (1:1500 dilution, Proteintech, Wuhan), anti-CYP7A1 polyclonal antibody (1:500 dilution, Bioss, Beijing, China), anti- LXR α polyclonal antibody (1:300 dilution, Proteintech, Wuhan, China), anti- SR-BImonoclonal antibody (1:500 dilution, Abcam, UK), anti-ABCG5 polyclonal antibody (1:500 dilution, Bioss, Beijing, China) and anti-ABCG8 polyclonal antibody (1:500 dilution, Bioss, Beijing, China).

Techniques: Real-time Polymerase Chain Reaction, Sequencing

The results of qPCR showed that high fat diet (HD) significantly decreased the expression of LXRα, ABCG5, ABCG8, SR-BI, CYP7A1 and LCAT,and CMS alone significantly elevated the expression of LXRα, ABCG5, ABCG8, and SR-BI, while CMS + HD significantly decreased the expression of ABCG5, ABCG8, CYP7A1 and LCAT. The data of mRNA expression were presented as mean ± SEM. * P < 0.05 and ** P < 0.01: compared to Control; ◊◊ P < 0.01: compared to HD group; ## P < 0.01: compared to CMS group, all with one-way ANOVA. The results of Western blot showed similar trends. The blots shown on gels respectively represented the group of Control, CMS, CMS + HD, HD.

Journal: Lipids in Health and Disease

Article Title: Chronic unpredictable mild stress combined with a high-fat diets aggravates atherosclerosis in rats

doi: 10.1186/1476-511X-13-77

Figure Lengend Snippet: The results of qPCR showed that high fat diet (HD) significantly decreased the expression of LXRα, ABCG5, ABCG8, SR-BI, CYP7A1 and LCAT,and CMS alone significantly elevated the expression of LXRα, ABCG5, ABCG8, and SR-BI, while CMS + HD significantly decreased the expression of ABCG5, ABCG8, CYP7A1 and LCAT. The data of mRNA expression were presented as mean ± SEM. * P < 0.05 and ** P < 0.01: compared to Control; ◊◊ P < 0.01: compared to HD group; ## P < 0.01: compared to CMS group, all with one-way ANOVA. The results of Western blot showed similar trends. The blots shown on gels respectively represented the group of Control, CMS, CMS + HD, HD.

Article Snippet: LCAT, CYP7A1, LXR α, SR-BI, ABCG5 and ABCG8 proteins were detected using an anti-LCAT polyclonal antibody (1:1500 dilution, Proteintech, Wuhan), anti-CYP7A1 polyclonal antibody (1:500 dilution, Bioss, Beijing, China), anti- LXR α polyclonal antibody (1:300 dilution, Proteintech, Wuhan, China), anti- SR-BImonoclonal antibody (1:500 dilution, Abcam, UK), anti-ABCG5 polyclonal antibody (1:500 dilution, Bioss, Beijing, China) and anti-ABCG8 polyclonal antibody (1:500 dilution, Bioss, Beijing, China).

Techniques: Expressing, Control, Western Blot