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Image Search Results
Journal: bioRxiv
Article Title: Quantitative comparison of fluorescent reporters by FCS excitation scan
doi: 10.64898/2026.04.04.716477
Figure Lengend Snippet: a Usable brightness and b usable laser power from FCS excitation scans of SNAP-Cell 505-Star, Oregon Green HALO ligand, Janelia Fluor 646 HALO ligand, and SNAP-Cell 647-SiR in solution at 37° C. c Confocal images of HEK293T cells expressing HALOtag9 or fSNAPtag and labelled with the indicated ligand. d Usable brightness and e usable laser power from FCS excitation scans of NLS-HALOtag9 and NLS-fSNAPtag expressed in HEK293T cells and labelled with SNAP-Cell 505-Star, Oregon Green HALO ligand, Janelia Fluor 646 HALO ligand, and SNAP-Cell 647-SiR at 37° C. Every dot is representative of one experiment (average). Horizontal bars are mean values and error bars are standard deviations. Adjusted p-values were obtained from one-way ANOVA with Tukey’s correction for multiple comparisons.
Article Snippet: Cells were labelled in full medium with HaloTag® Oregon Green® Ligand (Promega, G2802), Janelia Fluor® 646 HaloTag® Ligand (Promega, #HT1060),
Techniques: Expressing