Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Compound 1 eradicates MRSA (ATCC BAA 44) in the presence of light. A: Graph, showing a dose-dependent effect of 1 with and without a 2-minute irradiation with white light. B. Drip-streak plates utilized in the experiment illustrating the CFU counts. The data are derived from a single experiment performed in 3 replicates and serve as an example of 3 independent experiments producing similar results. The stars represent no observable colonies.

Article Snippet: To investigate whether the bactericidal effect of 1 was not limited to HA-MRSA (ATCC BAA-44), we performed experiments with other Gram-positive bacterial strains.

Techniques: Irradiation, Derivative Assay

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Bacterial patterning with light. Left: MRSA (ATCC BAA-44) cells pre-treated with 1 (1 μM), spread over an agar plate and irradiated with light through a mask for 2 minutes, and then incubated at 37 °C. NMT: New Mexico Tech. Right: Schematic of the bacterial patterning experiment.

Article Snippet: To investigate whether the bactericidal effect of 1 was not limited to HA-MRSA (ATCC BAA-44), we performed experiments with other Gram-positive bacterial strains.

Techniques: Irradiation, Incubation

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Compound 1 is effective against a variety of drug resistant Gram-positive bacterial strains in the presence of light. A. MRSA (ATCC BAA1717). B. S. aureus (ATCC 29213). C. E. faecalis (ATCC 51299). D. S. pyogenes (ATCC 8133). E. S. mutans (Ward’s 85W 2357). The data are derived from a single experiment performed in 3 replicates and serve as an example of 3 independent experiments producing similar results. The stars represent no observable colonies.

Article Snippet: To investigate whether the bactericidal effect of 1 was not limited to HA-MRSA (ATCC BAA-44), we performed experiments with other Gram-positive bacterial strains.

Techniques: Derivative Assay

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Eradication of MRSA (ATCC BAA44) by analogues of 1. The data are derived from a single experiment performed in 3 replicates and serve as an example of 3 independent experiments producing similar results. The stars represent no observable colonies.

Article Snippet: To investigate whether the bactericidal effect of 1 was not limited to HA-MRSA (ATCC BAA-44), we performed experiments with other Gram-positive bacterial strains.

Techniques: Derivative Assay

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: SEM images of MRSA (ATCC BAA44) cells treated with 1.

Article Snippet: To investigate whether the bactericidal effect of 1 was not limited to HA-MRSA (ATCC BAA-44), we performed experiments with other Gram-positive bacterial strains.

Techniques:

Journal: Bioorganic & medicinal chemistry letters

Article Title: Photoactivated 2,3-Distyrylindoles Kill Multi-Drug Resistant Bacteria

doi: 10.1016/j.bmcl.2018.04.001

Figure Lengend Snippet: Permeability of MRSA (ATCC BAA44) cell membrane probed by PI. Green fluorescence is due to 1. Red fluorescence is indicative of the disruption of cell membrane.

Article Snippet: To investigate whether the bactericidal effect of 1 was not limited to HA-MRSA (ATCC BAA-44), we performed experiments with other Gram-positive bacterial strains.

Techniques: Permeability, Fluorescence