Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Localisation of SSTRs in the cortical layers of mice primary somatosensory cortex. SSTR1 showed the most intense signal in L4 and a weaker signal in L2/3 and L5/6. SSTR2 was localised mainly in deep cortical layers (L5-6). SSTR3, SSTR4, and SSTR5 were distributed evenly throughout the cortical layers (column A and C). Column a present representative coronal brain sections through somatosensory cortex immunofluorescently labelled with antibodies specific for SSTR1-SSTR5. Column b presents the validation of antibody specificity. Coronal brain sections were treated with primary antibodies (anti-SSTR1-5) preincubated with control blocking peptides, followed by specific secondary antibodies. The addition of control peptides resulted in the disappearance of the antibody-specific signal. Scale bar 100 μm

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques: Blocking Assay

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: SSTRs localisation in the somatosensory cortex of mice. High magnification confocal pictures of coronal sections presenting localisation of SSTR1-5 in the mouse somatosensory cortex. a SSTR1 labelled clearly the pyramidal-like cell bodies and apical dendrites. b Lack of co-localisation of SSTR2s and PV INs in the mouse primary somatosensory cortex. Yellow lines represent two orthogonal sections of a z-series showing the distribution of green and red fluorescence in the tissue. c SSTR3 immunoreactivity was found in cell bodies and neuropil. d, e Immunofluorescent signal for SSTR4 and SSTR5 was found mainly in the neuropil and much less visible in cell bodies. Scale bar for all pictures: 10 μm

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques: Fluorescence

Journal: Brain Structure & Function

Article Title: Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex

doi: 10.1007/s00429-019-02011-7

Figure Lengend Snippet: Two types of SSTRs distribution in the mouse barrel field. Tangential brain sections through L4 of the mouse barrel cortex. Type A distribution is characterised by a high concentration of immunoreactivity in the barrel walls (sides + septa). This type is represented by SSTR1 and SSTR2. Type B distribution is characterised by a homogeneous intensity of immunoreactivity in the barrel hollows. This type is represented by SSTR3, SSTR4, and SSTR5. Scale bar: 100 μm. Nuclear DAPI staining is visible in blue

Article Snippet: The primary antibodies used in this study were as follows: rabbit anti-SSTR1 polyclonal antibody (1:400, catalogue no. ASR-001, Alomone Labs), rabbit anti-SSTR2 monoclonal antibody (1:2000, catalogue no. ab134152, Abcam), rabbit anti-SSTR3 polyclonal antibody (1:300, catalogue no. ASR-003, Alomone Labs), rabbit anti-SSTR4 polyclonal antibody (1:1000, catalogue no. ab28578, Abcam), and rabbit anti-SSTR5 polyclonal antibody (1:1000, catalogue no. ASR-005, Alomone Labs).

Techniques: Concentration Assay, Staining

Journal: Frontiers in immunology

Article Title: Somatostatin-SSTR3-GSK3 modulates human T-cell responses by inhibiting OXPHOS.

doi: 10.3389/fimmu.2024.1322670

Figure Lengend Snippet: FIGURE 5 SST modulates T-cell responses through SSTR3. (A) The expression of SSTR3 on fresh isolated T cells were measured by flow cytometry (number on the graph represent mean fluorescence intensity, MFI). T cells express SSTR3 at protein level (A, n=3). (B, C) Human purified T cells were labelled with CFSE and were pre-treated with SSTR3 inhibitor (SSTRi, MK-4256, 2nM) for 30min. Cells were then incubated with SST (100nM) for 30min. After that, T-cell activation beads were used to stimulate T cells at 1:2 ratio (beads: cells) for 4 days. T-cell proliferation were measured by the dilution of CFSE and quantified by flow cytometry. IFNg from T cells was measured by ELISA. The blockade of SSTR3 inhibits the effect of SST on T-cell proliferation (B), n=6) and IFNg production (C), n=6). (D-F) CFSE labeled human primary T cells were transfected with either control siRNA or SSTR3 siRNA mix. On day 2, the expression of SSTR3 were measured by FACS (D) After transfection, cells were pre-treated with SST for 30min and then stimulated with T-cell activation beads (aCD2, aCD3 and aCD28) for 4 days. CFSE dilution (E) and IFNg expression (F) was measured by flow cytometry and ELISA respectively (n=3). (G-I) Human purified T cells were pre-treated with SSTR3 inhibitor (SSTRi, MK-4256, 2nM) for 30min. Cells were then incubated with SST (100nM) for 30min. After that, T-cell activation beads were used to stimulate T cells at 1:2 ratio (beads: cells) for 24hrs. XF Mitochondria stress test was used to detect T-cell mitochondrial respiration. The blockade of SSTR3 reverses the effect of SST on T-cell mitochondrial respiration (G-I), n=4). Vehicle 1: ddH2O. Vehicle 2: DMSO. Data is presented as individual dots or mean±SD. Repeat measure two- way ANOVA, Two-stage step-up method of Benjamini, Krieger and Yekutieli to correct for multiple comparison by controlling the False Discovery Rate. ns, not significant, *p<0.05; **p<0.01; and ***p<0.001.

Article Snippet: Antibodies used for flow cytometry staining in this study include: CD3-PerCp Cy5.5 (SK7, BD Bioscience), CD4-APC-Cy7 (RPA-T4, BD Bioscience), CD8-PE-Cy7 (RPA-T8, BD Bioscience), SSTR3-Alexa 647 (FAB7018R, R&D system), CD25-PE (BC95, BD Bioscience), CD69-APC (FN50, BD Bioscience), GM-CSF-BV421 (BVD221C11, BD Bioscience), IL-17A-PE (N49-653, BD Bioscience), IL2-PE (5344.111, BD Bioscience) and IFNg-APC (B27, BD Bioscience).

Techniques: Expressing, Isolation, Cytometry, Incubation, Activation Assay, Enzyme-linked Immunosorbent Assay, Labeling, Transfection, Control, Comparison

Journal: Frontiers in immunology

Article Title: Somatostatin-SSTR3-GSK3 modulates human T-cell responses by inhibiting OXPHOS.

doi: 10.3389/fimmu.2024.1322670

Figure Lengend Snippet: FIGURE 6 SST modulates T-cell responses through GSK3. (A, B) Human purified T cells were pre-treated with SSTR3 inhibitor (SSTRi, MK-4256, 2nM) for 30min. Cells were then incubated with SST (100nM) for 30min. The phosphorylation of GSK3 (inactive form) and total GSK3 are measured using ELISA. SST decreases the phosphorylation of GSK3 (release the active form) (A), n=4) without change total GSK3 expression (B), n=4), which can be blocked by the inhibition of SSTR3 (A). (C, D) Human purified T cells were labelled with CFSE and were pre-treated with GSK3 inhibitor (GSK3i, CHIR 99021, 20nM) for 30min. Cells were then incubated with SST (100nM) for 30min. After that, T-cell activation beads were used to stimulate T cells at 1:2 ratio (beads: cells) for 4 days. T-cell proliferation were measured by the dilution of CFSE and quantified by flow cytometry. IFNg from T cells was measured by ELISA. The blockade of GSK3 inhibits the effect of SST on T-cell proliferation (C), n=7) and IFNg production (D), n=5). (E-G) CFSE labeled human primary T cells were transfected with either control siRNA or GSK3beta siRNA mix. On day 2, the expression of GSK3 were measured by ELISA (E). After transfection, cells were pre-treated with SST for 30min and were stimulated with T-cell activation beads (aCD2, aCD3 and aCD28) for 4 days. CFSE dilution and IFNg expression was measured by flow cytometry and ELISA respectively (n=3). (H-J) Human purified T cells were pre-treated with GSK3 inhibitor (GSK3i, CHIR 99021, 20nM) for 30min. Cells were then incubated with SST (100nM) for 30min. After that, T-cell activation beads were used to stimulate T cells at 1:2 ratio (beads: cells) for 24hrs. XF Mitochondria stress test was used to detect T-cell mitochondrial respiration. The blockade of GSK3 reverses the effect of SST on T-cell mitochondrial respiration (H-J), n=4). Vehicle 1: ddH2O. Vehicle 2: DMSO. Data is presented as individual dots or mean±SD. Repeat measure two-way ANOVA, Two-stage step-up method of Benjamini, Krieger and Yekutieli to correct for multiple comparison by controlling the False Discovery Rate. ns, not significant, *p<0.05 and **p<0.01.

Article Snippet: Antibodies used for flow cytometry staining in this study include: CD3-PerCp Cy5.5 (SK7, BD Bioscience), CD4-APC-Cy7 (RPA-T4, BD Bioscience), CD8-PE-Cy7 (RPA-T8, BD Bioscience), SSTR3-Alexa 647 (FAB7018R, R&D system), CD25-PE (BC95, BD Bioscience), CD69-APC (FN50, BD Bioscience), GM-CSF-BV421 (BVD221C11, BD Bioscience), IL-17A-PE (N49-653, BD Bioscience), IL2-PE (5344.111, BD Bioscience) and IFNg-APC (B27, BD Bioscience).

Techniques: Incubation, Phospho-proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Inhibition, Activation Assay, Cytometry, Labeling, Transfection, Control, Comparison

Journal: Cancers

Article Title: Somatostatin Receptors in Human Meningiomas—Clinicopathological Aspects

doi: 10.3390/cancers13225704

Figure Lengend Snippet: Antibodies.

Article Snippet: SSTR3 , Somatostatin R3/SSTR3 Antibody (polyclonal (rabbit), pH6, 1:1000 dilution, overnight incubation, somatostatin receptor 3, cat#NB100-74538, Novus Biologicals).

Techniques: Incubation

Journal: The EMBO Journal

Article Title: Somatostatin triggers local cAMP and Ca 2+ signaling in primary cilia to modulate pancreatic β-cell function

doi: 10.1038/s44318-025-00383-7

Figure Lengend Snippet: Reagents and tools table

Article Snippet: Rabbit anti-SSTR3 (1:200) , Elabscience , E-AB-16077.

Techniques: Recombinant, Plasmid Preparation, Sequencing, Virus, Cloning, Modification, Software, Microscopy, Real-time Polymerase Chain Reaction, SYBR Green Assay, Ligation

Journal: Scientific Reports

Article Title: Somatostatin receptor expression on von Hippel-Lindau-associated hemangioblastomas offers novel therapeutic target

doi: 10.1038/srep40822

Figure Lengend Snippet: Patients with von Hippel Lindau disease (VHL) undergoing surgical resection of posterior fossa hemangioblastoma provide confirmation of DOTATATE avidity of these lesions. The post-operative T1 weighted MRI image ( A ) and DOTATATE PET image ( B ) confirm complete resection of a DOTATATE avid posterior fossa hemangioblastoma ( C , D ). Representative images ( E ) of anti-SSTR IHC staining classified as negative (i), positive/negative (ii), positive (iii), double positive (iv) or triple positive (v). Scale bar = 50 μm. Average staining intensity for each SSTR subtype is shown in panel F. Number of tumors with SSTR expression are categorized by receptor subtype ( G ). Positive staining patterns were found in 8/9 tumors against SSTR1, in 8/9 tumors against SSTR2a, in 2/9 tumors against SSTR3, in 9/9 tumors against SSTR4, and in 8/9 tumors in SSTR5.

Article Snippet: Antibodies used were anti-Somatostatin Receptor 1 (SSTR1) (1:125, LS-C332380, Lifespan Bio, Seattle, WA), anti-Somatostatin Receptor 2a (SSTR2a) (1:500, PA3-109, Thermo Fischer Scientific Inc., Rockford, IL), anti-Somatostatin Receptor 3 (SSTR3) (1:100, TA323276, Origene, Rockville, MD), anti-Somatostatin Receptor 4 (SSTR4) (1:500, GTX70677, Genetex, Irvine, CA) anti-Somatostatin Receptor 5 (1:50, ab109495, Abcam, Cambridge, MA).

Techniques: Immunohistochemistry, Staining, Expressing

Journal: Scientific Reports

Article Title: Somatostatin receptor expression on von Hippel-Lindau-associated hemangioblastomas offers novel therapeutic target

doi: 10.1038/srep40822

Figure Lengend Snippet: A total of 9 VHL-HBs were stained against somatostatin receptor subtypes 1, 2a, 3, 4, and 5.

Article Snippet: Antibodies used were anti-Somatostatin Receptor 1 (SSTR1) (1:125, LS-C332380, Lifespan Bio, Seattle, WA), anti-Somatostatin Receptor 2a (SSTR2a) (1:500, PA3-109, Thermo Fischer Scientific Inc., Rockford, IL), anti-Somatostatin Receptor 3 (SSTR3) (1:100, TA323276, Origene, Rockville, MD), anti-Somatostatin Receptor 4 (SSTR4) (1:500, GTX70677, Genetex, Irvine, CA) anti-Somatostatin Receptor 5 (1:50, ab109495, Abcam, Cambridge, MA).

Techniques: Staining

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of the quantitative SST and SST1-5 mRNA expression in CRC patients and in histologically normal colorectal mucosa from the same patients (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between reciprocal expression of SST and SST1-5 mRNA in colorectal cancer samples (CRC) and non-tumor samples (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Incidence of somatostatin (SST) and SST receptor (SST1-5) expression in colorectal cancer (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Detection of IHC expression pattern of SST receptors (SST1-5) in colorectal cancer (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of the quantitative SST and SST1-5 immunoexpression in colorectal cancer (CRC), lymph node metastasis (LNM), and control mucosa (C) (A%).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between reciprocal immunoexpression of SST and SST1-5 in colorectal cancer samples (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST1-5 expression in relation to different IHC patterns of cellular localization.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between SST and or SST1-5 (mRNA and peptide) in colorectal cancer (CRC) and control mucosa samples (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST1-5 immunoexpression in colorectal cancer (CRC) and control samples (C) in relation to different anatomical sites of the large intestine.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST and SST1-5 mRNA expression in colorectal cancer (CRC) in relation to mucinous vs. nonmucinous subtypes of CRC.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST and SST1-5 immunoexpression in colorectal cancer (CRC) in relation to mucinous vs. nonmucinous CRC subtypes.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between SST and SST1-5 mRNA in colorectal cancer (CRC), control colorectal mucosa (C), age of the patients, and basic laboratory tests.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control