srii Search Results


usp xxx paddle apparatus hanson sr ii 6 flask dissolution test station  (Hanson Research Corporation)
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Hanson Research Corporation usp xxx paddle apparatus hanson sr ii 6 flask dissolution test station
Usp Xxx Paddle Apparatus Hanson Sr Ii 6 Flask Dissolution Test Station, supplied by Hanson Research Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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srii flow cytometer  (Becton Dickinson)
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Becton Dickinson srii flow cytometer
Srii Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flask dissolution test station sr8 srii  (Hanson Research Corporation)
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Hanson Research Corporation flask dissolution test station sr8 srii
Flask Dissolution Test Station Sr8 Srii, supplied by Hanson Research Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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photoisomerization of retinal in the buried photoactive site of srii  (BlueLight analytics inc)
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BlueLight analytics inc photoisomerization of retinal in the buried photoactive site of srii
Photoisomerization Of Retinal In The Buried Photoactive Site Of Srii, supplied by BlueLight analytics inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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srii flow cytometry  (Becton Dickinson)
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Becton Dickinson srii flow cytometry
Robust RFP1 loss by transient expression of AID. ( A ) 293FT/RFP1 cells transiently transfected with plasmids encoding AID mutants, selected with puromycin for 3 days and analyzed for somatic hypermutation by RFP fluorescence loss. Results show mean values of three experiments ± SD. ( B ) Representative flow <t>cytometry</t> results and ( C ) analysis of accumulative RFP loss after one and two rounds of transient AID expression. Results show the mean values of three replicates ± SD. Significant differences between RFP loss after the first and the second AID transfections are indicated; **P ≤ 0.01, ***P ≤ 0.001.
Srii Flow Cytometry, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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elisa based on the tgf-h srii capture reagent  (Grainger Industrial)
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Grainger Industrial elisa based on the tgf-h srii capture reagent
Robust RFP1 loss by transient expression of AID. ( A ) 293FT/RFP1 cells transiently transfected with plasmids encoding AID mutants, selected with puromycin for 3 days and analyzed for somatic hypermutation by RFP fluorescence loss. Results show mean values of three experiments ± SD. ( B ) Representative flow <t>cytometry</t> results and ( C ) analysis of accumulative RFP loss after one and two rounds of transient AID expression. Results show the mean values of three replicates ± SD. Significant differences between RFP loss after the first and the second AID transfections are indicated; **P ≤ 0.01, ***P ≤ 0.001.
Elisa Based On The Tgf H Srii Capture Reagent, supplied by Grainger Industrial, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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the his-tagged n.p.srii  (Qiagen)
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Qiagen the his-tagged n.p.srii
Robust RFP1 loss by transient expression of AID. ( A ) 293FT/RFP1 cells transiently transfected with plasmids encoding AID mutants, selected with puromycin for 3 days and analyzed for somatic hypermutation by RFP fluorescence loss. Results show mean values of three experiments ± SD. ( B ) Representative flow <t>cytometry</t> results and ( C ) analysis of accumulative RFP loss after one and two rounds of transient AID expression. Results show the mean values of three replicates ± SD. Significant differences between RFP loss after the first and the second AID transfections are indicated; **P ≤ 0.01, ***P ≤ 0.001.
The His Tagged N.P.Srii, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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srii instrument  (Becton Dickinson)
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Becton Dickinson srii instrument
Robust RFP1 loss by transient expression of AID. ( A ) 293FT/RFP1 cells transiently transfected with plasmids encoding AID mutants, selected with puromycin for 3 days and analyzed for somatic hypermutation by RFP fluorescence loss. Results show mean values of three experiments ± SD. ( B ) Representative flow <t>cytometry</t> results and ( C ) analysis of accumulative RFP loss after one and two rounds of transient AID expression. Results show the mean values of three replicates ± SD. Significant differences between RFP loss after the first and the second AID transfections are indicated; **P ≤ 0.01, ***P ≤ 0.001.
Srii Instrument, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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srii facs machine  (Becton Dickinson)
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Becton Dickinson srii facs machine
Robust RFP1 loss by transient expression of AID. ( A ) 293FT/RFP1 cells transiently transfected with plasmids encoding AID mutants, selected with puromycin for 3 days and analyzed for somatic hypermutation by RFP fluorescence loss. Results show mean values of three experiments ± SD. ( B ) Representative flow <t>cytometry</t> results and ( C ) analysis of accumulative RFP loss after one and two rounds of transient AID expression. Results show the mean values of three replicates ± SD. Significant differences between RFP loss after the first and the second AID transfections are indicated; **P ≤ 0.01, ***P ≤ 0.001.
Srii Facs Machine, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pbii niii srii go(o)1 go(o)2 go(oh)1 go(oh)2  (Verlag GmbH)
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Verlag GmbH pbii niii srii go(o)1 go(o)2 go(oh)1 go(oh)2
Robust RFP1 loss by transient expression of AID. ( A ) 293FT/RFP1 cells transiently transfected with plasmids encoding AID mutants, selected with puromycin for 3 days and analyzed for somatic hypermutation by RFP fluorescence loss. Results show mean values of three experiments ± SD. ( B ) Representative flow <t>cytometry</t> results and ( C ) analysis of accumulative RFP loss after one and two rounds of transient AID expression. Results show the mean values of three replicates ± SD. Significant differences between RFP loss after the first and the second AID transfections are indicated; **P ≤ 0.01, ***P ≤ 0.001.
Pbii Niii Srii Go(O)1 Go(O)2 Go(Oh)1 Go(Oh)2, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Robust RFP1 loss by transient expression of AID. ( A ) 293FT/RFP1 cells transiently transfected with plasmids encoding AID mutants, selected with puromycin for 3 days and analyzed for somatic hypermutation by RFP fluorescence loss. Results show mean values of three experiments ± SD. ( B ) Representative flow cytometry results and ( C ) analysis of accumulative RFP loss after one and two rounds of transient AID expression. Results show the mean values of three replicates ± SD. Significant differences between RFP loss after the first and the second AID transfections are indicated; **P ≤ 0.01, ***P ≤ 0.001.

Journal: Scientific Reports

Article Title: Transient AID expression for in situ mutagenesis with improved cellular fitness

doi: 10.1038/s41598-018-27717-2

Figure Lengend Snippet: Robust RFP1 loss by transient expression of AID. ( A ) 293FT/RFP1 cells transiently transfected with plasmids encoding AID mutants, selected with puromycin for 3 days and analyzed for somatic hypermutation by RFP fluorescence loss. Results show mean values of three experiments ± SD. ( B ) Representative flow cytometry results and ( C ) analysis of accumulative RFP loss after one and two rounds of transient AID expression. Results show the mean values of three replicates ± SD. Significant differences between RFP loss after the first and the second AID transfections are indicated; **P ≤ 0.01, ***P ≤ 0.001.

Article Snippet: Cells were washed with phosphate buffered saline (PBS) and then incubated with 1 μM Sytox Blue viability dye (Thermo Fisher Scientific) in PBS containing 0.05% bovine serum albumin (BSA) at room temperature for 30 minutes before measurement on a SRII flow cytometry (Becton Dickinson, Mountain View, CA, USA), with analysis by FlowJo software (Tree Star, San Carlos, CA, USA).

Techniques: Expressing, Transfection, Fluorescence, Flow Cytometry