Journal: Frontiers in Microbiology

Article Title: Detection of Cronobacter Genus in Powdered Infant Formula by Enzyme-linked Immunosorbent Assay Using Anti- Cronobacter Antibody

doi: 10.3389/fmicb.2016.01124

Figure Lengend Snippet: Cross-reactivity of the developed indirect non-competitive enzyme-linked immunosorbent assay with non- Cronobacter species in pure culture.

Article Snippet: B. cereus (KCCM 40935), Buttiauxella noackiae (ATCC 51713), C. condimenti (LMG 26250), C. dublinensis (LMG 23823), C. malonaticus (LMG 23826), C. muytjensii (CDC 3523-75), C. turicensis (LMG 23827), C. muytjensii (ATCC 51329), C. sakazakii (ATCC 29544), C. sakazakii (ATCC 29004), C. universalis (LMG 26249), C. freundi (ATCC 8090), Escherichia coli (ATCC 39418), Franconibacter helveticus (LMG 23732), Franconibacter pulveris (LMG 24057), and Salmonella Typhimurium (ATCC 13311) were used in this study.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Experimental & Molecular Medicine

Article Title: Flightless-1 inhibits ER stress-induced apoptosis in colorectal cancer cells by regulating Ca 2+ homeostasis

doi: 10.1038/s12276-020-0448-3

Figure Lengend Snippet: a Sorcin expression levels were analyzed by western blot and quantitative real-time PCR. Data are shown as the mean ± SEM ( n = 3). *** p < 0.001, shRNA-Ctrl cells vs. shRNA-FliI cells. b After cotransfection of vector or sorcin with D1ER, FRET signals were measured. Data are shown as the mean ± SEM. *** p < 0.001, shRNA-Ctrl cells vs. shRNA-FliI cells. c Ctrl- and FliI-KD cells were treated with 50 nM TG for 6 h following expression of vector or sorcin. Cell lysates were analyzed by western blotting for phospho-PERK, phospho-IREα, GRP78/BiP, CHOP, sorcin, FliI, and tubulin.

Article Snippet: Mouse sorcin expression plasmids were purchased from Sino Biological (BDA, Beijing, China).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, shRNA, Cotransfection, Plasmid Preparation

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A , B The expression of ANXA7 and SRI in different cancers or specific cancer subtypes was analyzed through TIMER2. The expression of ANXA7 and SRI in HCC are higher ( n = 371) than normal liver tissues ( n = 50) (*** p < 0.001). C , D The expression of ANXA7 is higher in HCC ( n = 369) than in normal liver tissues ( n = 160) from the TCGA database. The expression of SRI is also higher in HCC ( n = 369) than in normal liver tissues ( n = 160) from the TCGA database ( * p < 0.05). E – G The expression of ANXA7 and SRI were higher in Huh-7/Hep3B cells than in normal human hepatocytes HL-7702 by qRT-PCR and western blotting. H Representative images of ANXA7 and SRI expression in normal human liver tissues and HCC tissues. I Quantification of the average means optical density for ANXA7 and SRI in normal liver tissues and HCC tissues (** p < 0.01, * p < 0.05). J ANXA7 expression level p ositively correlated with SRI expression in HCC cells ( r 2 = 0.882, p < 0.01). K ANXA7 expression level positively correlated with SRI expression in HCC tissues ( r 2 = 0.707, p < 0.01).

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A There may be a potential interaction between ANXA7 and SRI, which was analyzed by the STRING database. B co-IP assay showed the interaction between ANXA7 and SRI in Huh-7/Hep3B cells. C Cellular immunofluorescence proved that ANXA7 and SRI are colocalized in the cytoplasm and cell membrane in Huh-7/Hep3B cells.

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Co-Immunoprecipitation Assay, Immunofluorescence, Membrane

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A – D Upregulated ANXA7 increased SRI expression and downregulated ANXA7 decreased SRI expression at the mRNA level in Huh-7 and Hep3B cells (** p < 0.01,* p < 0.05). E – H Upregulated ANXA7 increased SRI expression at the protein level and promoted EMT, whereas downregulated ANXA7 decreased SRI expression at the protein level and inhibited EMT in Huh-7 and Hep3B cells (** p < 0.01,* p < 0.05). I – J IF was used to detect E-cadherin and cytokeratin. K , L IF assay was used to detect the N-cadherin and Vimentin in Huh-7/Hep3B cells.

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A , B Images of tumor xenografts and tumor weight (** p < 0.01). C , D The qRT-PCR analysis of ANXA7 and SRI in tumor xenografts (** p < 0.01). E , F Western blotting to detect ANXA7, SRI, E-cadherin, Cytokeratin, N-cadherin, and Vimentin in tumor xenografts (** p < 0.01, * p < 0.05). G , H Immunohistochemical analysis of ANXA7, SRI and EMT indicators in tumor xenografts (** p < 0.01, * p < 0.05).

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Quantitative RT-PCR, Western Blot, Immunohistochemical staining

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: A – D When SRI is upregulated, the expression of ANXA7 increased significantly, while when SRI is downregulated, the expression of ANXA7 also decreased in Huh-7 and Hep3B cells by qRT-PCR (** p < 0.01, * p < 0.05). E – H When SRI is upregulated, the expression of ANXA7 increased, and promoted EMT, whereas when SRI is downregulated, the expression of ANXA7 also decreased, and inhibited EMT in Huh-7 and Hep3B cells by western blotting (** p < 0.01, * p < 0.05). I , J IF was used to detect the E-cadherin and Cytokeratin after upregulating and downregulating SRI. K , L IF was used to detect the N-cadherin and Vimentin in Huh-7/Hep3B cells after upregulating and downregulating SRI.

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques: Expressing, Quantitative RT-PCR, Western Blot

Journal: Cell Death & Disease

Article Title: AnnexinA7 promotes epithelial–mesenchymal transition by interacting with Sorcin and contributes to aggressiveness in hepatocellular carcinoma

doi: 10.1038/s41419-021-04287-2

Figure Lengend Snippet: Interaction between ANXA7 and SRI affects EMT progress and contributes to the aggressiveness in HCC.

Article Snippet: The specific antibodies including ANXA7 (Abcam Cambridge, MA, USA), 1:500; SRI (Proteintech, Wuhan, China), 1:500; E-cadherin (Proteintech, Wuhan, China), 1:500; Cytokeratin (Proteintech, Wuhan, China), 1:500; N-cadherin (Proteintech, Wuhan, China), 1:500; Vimentin (Proteintech, Wuhan, China), 1:500; GAPDH (Proteintech, Wuhan, China) 1:1000.

Techniques:

Journal: Scientific Reports

Article Title: RETRACTED ARTICLE: Sorcin Predicts Poor Prognosis and Promotes Metastasis by Facilitating Epithelial-mesenchymal Transition in Hepatocellular Carcinoma

doi: 10.1038/s41598-017-10365-3

Figure Lengend Snippet: Sorcin promotes HCC cell invasion and metastasis in vitro and in vivo . (A&B) Sorcin promoted HCC cell migration and invasion in vitro . Transwell migration ( A ) and invasion ( B ) assays of HepG2 cells with Sorcin overexpression (HepG2 Sorcin ) or control (HepG2 Vector ), and HCCLM3 cells with Sorcin knockdown (HCCLM3 shSorcin ) or control (HCCLM3 shcontrol ). ( C , D ) Sorcin promoted HCC cell growth in vivo . ( C ) Left column: Linear graphs were used to analyze the rate of tumor growth in each group. Middle column: Representative subcutaneous tumors from HepG2 Sorcin and HCCLM3 shSorcin cells and their control cells were shown in the middle panel. Right column: Tumor volumes of each group were shown and compared in the bar charts. ( D ) Left and middle column: Representative pictures of orthotopic liver exnograft tumors from each indicated groups were shown. Right column: Tumor volumes of each group were shown and compared in the bar charts. ( E ) Representative pictures of intrahepatic ( E1 ) and lung metastasis ( E2) . original magnification: left, 100×; right, 400×. ( F ) The number of metastatic nodules in each mice liver ( F1 ) or lung ( F2 ) was calculated and compared. *** P < 0.001. Error bars, SD.

Article Snippet: The lentiviral vectors (LV) encoding short hairpin RNAs (shRNAs) for Sorcin knockdown, and LV encoding Sorcin gene were purchased from OriGene Technologies Incorporation (Rockville, MD).

Techniques: In Vitro, In Vivo, Migration, Over Expression, Control, Plasmid Preparation, Knockdown

Journal: Scientific Reports

Article Title: RETRACTED ARTICLE: Sorcin Predicts Poor Prognosis and Promotes Metastasis by Facilitating Epithelial-mesenchymal Transition in Hepatocellular Carcinoma

doi: 10.1038/s41598-017-10365-3

Figure Lengend Snippet: Sorcin Enhances Metastasis by Activating ERK Signaling via Facilitating EMT. ( A ) Representative phase contrast images for cell morphology showed that Sorcin affected the HCC cell morphology ( A1 ) and F-actin immunofluorescence staining was used to analyze the cytoskeleton in HepG2 Sorcin , HCCLM3 shSorcin and their control cells ( A2 ). ( B ) Immunofluorescent (IF) showed the relative expression of E-cadherin (red), Vimentin (green) in HepG2 Sorcin with Sorcin overexpression or HCCLM3 shSorcin cells with Sorcin knockdown, their corresponding control cells. ( C ) Western blot analysis of E-cadherin and vimentin expressions in HepG2 Sorcin , HCCLM3 shSorcin and their control cells with/without U0126 treatment. ( D ) Representative IHC images of Sorcin, p-ERK, E-cadherin and Vimentin expression in HCC tissues. Magnification: 100×.

Article Snippet: The lentiviral vectors (LV) encoding short hairpin RNAs (shRNAs) for Sorcin knockdown, and LV encoding Sorcin gene were purchased from OriGene Technologies Incorporation (Rockville, MD).

Techniques: Immunofluorescence, Staining, Control, Expressing, Over Expression, Knockdown, Western Blot