Journal: RSC Advances

Article Title: Beneficial roles of the AhR ligand FICZ on the regenerative potentials of BMSCs and primed cartilage templates †

doi: 10.1039/d2ra00622g

Figure Lengend Snippet: Effects of FICZ or SR1 on proliferation, osteogenic differentiation, cell migration and inflammatory responses of the cultured BMSCs. (a) The absorbance at 450 nm of the CCK8 assay of the BMSCs. The number of viable BMSCs increased in all groups and there were no significant differences among the three groups on days 0, 1, 3 and 5 ( p < 0.05). (b) ALP staining (upper) and ARS staining (lower) showed a stimulative effect of FICZ and inhibitory effect of SR1 on the osteogenic differentiation of BMSCs. (c) RT-PCR demonstrated that FICZ or SR1 prevented or exacerbated LPS-induced IL-1β, 6 and TNF-α, respectively (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: BMSCs were seeded in 96-well plate (1 × 10 3 cells per well) and incubated in a culture medium containing 500 nM FICZ (CAS No. 172922-91-7) (HY-12451, MCE), 1 μM SR1 (CAS No. 1227633-49-9) (HY-15001, MCE) or dimethyl sulphoxide (DMSO) as a control.

Techniques: Migration, Cell Culture, CCK-8 Assay, Staining, Reverse Transcription Polymerase Chain Reaction

Journal: Current oncology (Toronto, Ont.)

Article Title: TM7SF2 as a Potential Biomarker in Colorectal Cancer: Implications for Metastasis.

doi: 10.3390/curroncol32020114

Figure Lengend Snippet: Figure 1. TM7SF2 overexpression and its association with poor prognosis in CRC patients. (A) Im- munohistochemistry assays demonstrate higher TM7SF2 expression levels in tumor tissues compared to normal (adjacent tumor) tissues. (B) A correlation between TM7SF2 overexpression and higher clinical stages in CRC patients (n = 234, *** p < 0.001). (C) Association of TM7SF2 expression with the overall survival of CRC patients, as illustrated by the Kaplan–Meier curve.

Article Snippet: The membrane was blocked with 5% BSA solution for 1 h and incubated with antihuman TM7SF2 polyclonal rabbit antibody (Proteintech, Rosemont, IL, USA, 1:1000) overnight at 4 ◦C.

Techniques: Over Expression, Expressing

Journal: Current oncology (Toronto, Ont.)

Article Title: TM7SF2 as a Potential Biomarker in Colorectal Cancer: Implications for Metastasis.

doi: 10.3390/curroncol32020114

Figure Lengend Snippet: Figure 2. Confirmation of TM7SF2 expression after siRNA transfection through RT-PCR and Western blot. (A) mRNA expression levels of TM7SF2 in CRC cells (SW480 and SW620) post-TM7SF2 knockdown, as confirmed by RT-PCR. (B) Comparison of TM7SF2 expression in the knockdown group relative to the negative control, normalized using GAPDH. (C) Protein expression levels of TM7SF2 in CRC cells (SW480 and SW620) after siRNA transfection, evaluated by Western blot. (D) Comparison of TM7SF2 expression in the knockdown group to the negative control, quantified using β-actin (*** p < 0.001). The uncropped blots are shown in the Supplemental Materials.

Article Snippet: The membrane was blocked with 5% BSA solution for 1 h and incubated with antihuman TM7SF2 polyclonal rabbit antibody (Proteintech, Rosemont, IL, USA, 1:1000) overnight at 4 ◦C.

Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Knockdown, Comparison, Negative Control

Journal: Current oncology (Toronto, Ont.)

Article Title: TM7SF2 as a Potential Biomarker in Colorectal Cancer: Implications for Metastasis.

doi: 10.3390/curroncol32020114

Figure Lengend Snippet: Figure 3. TM7SF2 knockdown reduces the proliferation of CRC cells, as evidenced by the WST-1 assay. (A) Measurement of absorbance at 450 nm over time in SW480 cells. (B) Comparison of the proliferation rate in the TM7SF2 knockdown group to the SW480 negative control. (C) Measurement of absorbance in SW620 cells following WST-1 treatment. (D) Comparison of the proliferation rate in the TM7SF2 knockdown group to the SW620 negative control (*** p < 0.001).

Article Snippet: The membrane was blocked with 5% BSA solution for 1 h and incubated with antihuman TM7SF2 polyclonal rabbit antibody (Proteintech, Rosemont, IL, USA, 1:1000) overnight at 4 ◦C.

Techniques: Knockdown, WST-1 Assay, Comparison, Negative Control

Journal: Current oncology (Toronto, Ont.)

Article Title: TM7SF2 as a Potential Biomarker in Colorectal Cancer: Implications for Metastasis.

doi: 10.3390/curroncol32020114

Figure Lengend Snippet: Figure 4. The Transwell analysis demonstrates reduced migration and invasion of CRC cells following TM7SF2 downregulation. (A,B) Migration of CRC cells to the bottom chamber was inhibited in the TM7SF2 knockdown group compared to the negative controls; (A) SW480 and (B) SW620. (C,D) In- vasion into the bottom chamber was similarly inhibited in the TM7SF2 knockdown group over the negative controls; (C) SW480 and (D) SW620. (*** p < 0.001, scale bar: 100 µm at 100× magnification).

Article Snippet: The membrane was blocked with 5% BSA solution for 1 h and incubated with antihuman TM7SF2 polyclonal rabbit antibody (Proteintech, Rosemont, IL, USA, 1:1000) overnight at 4 ◦C.

Techniques: Migration, Knockdown

Journal: Current oncology (Toronto, Ont.)

Article Title: TM7SF2 as a Potential Biomarker in Colorectal Cancer: Implications for Metastasis.

doi: 10.3390/curroncol32020114

Figure Lengend Snippet: Figure 5. TM7SF2 downregulation decreases colony-formation in CRC cells as demonstrated by the soft agar colony forming assay. (A,B) Colony formation was inhibited in CRC cells with TM7SF2 knockdown compared to the negative control; (A) SW480 and (B) SW620. (** p < 0.01, Scale bar: 100 µm (40× magnification)).

Article Snippet: The membrane was blocked with 5% BSA solution for 1 h and incubated with antihuman TM7SF2 polyclonal rabbit antibody (Proteintech, Rosemont, IL, USA, 1:1000) overnight at 4 ◦C.

Techniques: Knockdown, Negative Control

Journal: Scientific Reports

Article Title: RETRACTED ARTICLE: Sorcin Predicts Poor Prognosis and Promotes Metastasis by Facilitating Epithelial-mesenchymal Transition in Hepatocellular Carcinoma

doi: 10.1038/s41598-017-10365-3

Figure Lengend Snippet: Sorcin promotes HCC cell invasion and metastasis in vitro and in vivo . (A&B) Sorcin promoted HCC cell migration and invasion in vitro . Transwell migration ( A ) and invasion ( B ) assays of HepG2 cells with Sorcin overexpression (HepG2 Sorcin ) or control (HepG2 Vector ), and HCCLM3 cells with Sorcin knockdown (HCCLM3 shSorcin ) or control (HCCLM3 shcontrol ). ( C , D ) Sorcin promoted HCC cell growth in vivo . ( C ) Left column: Linear graphs were used to analyze the rate of tumor growth in each group. Middle column: Representative subcutaneous tumors from HepG2 Sorcin and HCCLM3 shSorcin cells and their control cells were shown in the middle panel. Right column: Tumor volumes of each group were shown and compared in the bar charts. ( D ) Left and middle column: Representative pictures of orthotopic liver exnograft tumors from each indicated groups were shown. Right column: Tumor volumes of each group were shown and compared in the bar charts. ( E ) Representative pictures of intrahepatic ( E1 ) and lung metastasis ( E2) . original magnification: left, 100×; right, 400×. ( F ) The number of metastatic nodules in each mice liver ( F1 ) or lung ( F2 ) was calculated and compared. *** P < 0.001. Error bars, SD.

Article Snippet: The lentiviral vectors (LV) encoding short hairpin RNAs (shRNAs) for Sorcin knockdown, and LV encoding Sorcin gene were purchased from OriGene Technologies Incorporation (Rockville, MD).

Techniques: In Vitro, In Vivo, Migration, Over Expression, Control, Plasmid Preparation, Knockdown

Journal: Scientific Reports

Article Title: RETRACTED ARTICLE: Sorcin Predicts Poor Prognosis and Promotes Metastasis by Facilitating Epithelial-mesenchymal Transition in Hepatocellular Carcinoma

doi: 10.1038/s41598-017-10365-3

Figure Lengend Snippet: Sorcin Enhances Metastasis by Activating ERK Signaling via Facilitating EMT. ( A ) Representative phase contrast images for cell morphology showed that Sorcin affected the HCC cell morphology ( A1 ) and F-actin immunofluorescence staining was used to analyze the cytoskeleton in HepG2 Sorcin , HCCLM3 shSorcin and their control cells ( A2 ). ( B ) Immunofluorescent (IF) showed the relative expression of E-cadherin (red), Vimentin (green) in HepG2 Sorcin with Sorcin overexpression or HCCLM3 shSorcin cells with Sorcin knockdown, their corresponding control cells. ( C ) Western blot analysis of E-cadherin and vimentin expressions in HepG2 Sorcin , HCCLM3 shSorcin and their control cells with/without U0126 treatment. ( D ) Representative IHC images of Sorcin, p-ERK, E-cadherin and Vimentin expression in HCC tissues. Magnification: 100×.

Article Snippet: The lentiviral vectors (LV) encoding short hairpin RNAs (shRNAs) for Sorcin knockdown, and LV encoding Sorcin gene were purchased from OriGene Technologies Incorporation (Rockville, MD).

Techniques: Immunofluorescence, Staining, Control, Expressing, Over Expression, Knockdown, Western Blot

Journal: Neuropharmacology

Article Title: Support for 5-HT2C receptor functional selectivity in vivo utilizing structurally diverse, selective 5-HT2C receptor ligands and the 2,5-dimethoxy-4-iodoamphetamine elicited head-twitch response model.

doi: 10.1016/j.neuropharm.2013.01.007

Figure Lengend Snippet: Fig. 1. The effects of commercially available 5-HT2C agonists (A) 4-phenyl-2- aminotetralin 5-HT2C agonists (B) and selective 5-HT2 antagonists (C) on the HTR elicited by DOI (1 mg/kg). The dose (mg/kg) is indicated in parentheses. “2nd” refers to test sessions in which the antagonist was administered after DOI. *Indicates a statis- tically significant difference from DOI alone. þIndicates a statistically significant dif- ference from the other 5-HT2C agonists. #Indicates a statistically significant difference from lower doses.

Article Snippet: During the verification process, it was observed that the mouse htr2C cDNA from Origene coded for the VNV edited isoform of this receptor. cDNA coding for the human 5- HT2A or 5-HT2C-INI receptors were from previously transformed competent cells, as previously reported (Canal et al., 2011).

Techniques:

Journal: Neuropharmacology

Article Title: Support for 5-HT2C receptor functional selectivity in vivo utilizing structurally diverse, selective 5-HT2C receptor ligands and the 2,5-dimethoxy-4-iodoamphetamine elicited head-twitch response model.

doi: 10.1016/j.neuropharm.2013.01.007

Figure Lengend Snippet: Fig. 2. The effects of commercially available 5-HT2C agonists (A), 4-phenyl-2- aminotetralin 5-HT2C agonists (B), and selective 5-HT2 antagonists (C) on locomo- tion following administration of saline, DOI (1 mg/kg), or DOI in combination with the various compounds. The dose (mg/kg) is indicated in parentheses. “2nd” refers to test sessions in which the antagonist was administered after DOI. *Indicates a statistically significant difference from DOI alone. þIndicates a statistically significant difference from vehicle alone.

Article Snippet: During the verification process, it was observed that the mouse htr2C cDNA from Origene coded for the VNV edited isoform of this receptor. cDNA coding for the human 5- HT2A or 5-HT2C-INI receptors were from previously transformed competent cells, as previously reported (Canal et al., 2011).

Techniques: Saline