Journal: Communications Biology

Article Title: Multiplexed proteomic biosensor platform for label-free real-time simultaneous kinetic screening of thousands of protein interactions

doi: 10.1038/s42003-025-07844-z

Figure Lengend Snippet: a Image of the SPOC sensor surface (10 k pattern) as viewed from the flowcell of the OpenPleX. The hockey-stick shaped orientation mark is visible in the center of the array. b Difference image showing the pattern of binding observed on the SPOC biosensor surface after injection of mouse anti-HaloTag antibody (133 nM). c Difference image showing the pattern of binding observed after the Mouse anti-Jun antibody injection (200 nM) which specifically detects two spots (yellow marks) where Jun-HaloTag was capture-purified as expected. d 1:1 fit (orange line) OpenPleX data of the anti-Jun antibody binding to Jun Spots #1 and #2 from c show similar binding profiles and equilibrium dissociation constants. e Difference image shows the pattern of binding observed after the mouse anti-p53 antibody injection (17.8 nM) which specifically detects four spots (yellow marks) where p53-HaloTag was capture-purified as expected. f 1:1 fit (orange lines) OpenPleX data of the anti-p53 antibody binding to the four p53 spots detected in e show similar binding profiles and equilibrium dissociation constants. g Iso-affinity plot of the kinetics measured for the Mouse anti-p53 and anti-Jun injections (p53 = 10 spots across 3 sensors; Jun = 4 spots across 2 sensors). The numerical source dataset for this iso-affinity plot is on Table .

Article Snippet: The SPOC technology is adaptable to any SPR instrument; however, for this publication, the custom Carterra LSA XT SPR and Horiba Scientific OpenPleX SPR imaging (SPRi) instruments were used for validation because both instruments are amenable to HTP screening and require minimal effort to integrate with our SPOC protein biosensor platform.

Techniques: Binding Assay, Injection, Purification

Journal: Communications Biology

Article Title: Multiplexed proteomic biosensor platform for label-free real-time simultaneous kinetic screening of thousands of protein interactions

doi: 10.1038/s42003-025-07844-z

Figure Lengend Snippet: 10 k SPOC array kinetic parameters derived from two protein specific antibody analytes binding to their respective target protein-ligands as measured on the OpenPleX instrument

Article Snippet: The SPOC technology is adaptable to any SPR instrument; however, for this publication, the custom Carterra LSA XT SPR and Horiba Scientific OpenPleX SPR imaging (SPRi) instruments were used for validation because both instruments are amenable to HTP screening and require minimal effort to integrate with our SPOC protein biosensor platform.

Techniques: Derivative Assay, Binding Assay

Journal: Biosensors

Article Title: An Overview of Artificial Olfaction Systems with a Focus on Surface Plasmon Resonance for the Analysis of Volatile Organic Compounds

doi: 10.3390/bios11080244

Figure Lengend Snippet: Schematic representation of the SPR-based olfactory biosensor with odorant binding proteins (OBPs) as sensing elements. The three rat OBP3 mutants were immobilized on the gold surface of a prism and their interaction with volatile organic compounds (VOCs) was monitored by SPR imaging.

Article Snippet: The SPR measurements were performed using a commercial SPR imaging apparatus (SRRiPlex from Horiba).

Techniques: Binding Assay, Imaging

Journal: Biosensors

Article Title: An Overview of Artificial Olfaction Systems with a Focus on Surface Plasmon Resonance for the Analysis of Volatile Organic Compounds

doi: 10.3390/bios11080244

Figure Lengend Snippet: Schematic presentation of the home-made SPR imaging setup.

Article Snippet: The SPR measurements were performed using a commercial SPR imaging apparatus (SRRiPlex from Horiba).

Techniques: Imaging

Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Plasmofluidics: Merging Light and Fluids at the Micro-/Nano-Scale

doi: 10.1002/smll.201500970

Figure Lengend Snippet: (a) Photo of SPR imaging integrated with a microfluidic system. (b)–(f) Consecutive photos of the SPR imaging chip that is carrying out immunoreactions in the microfluidic system. (b) Vertical reagent injection step: a reagent (yellow liquid) is injected into the flow channels and then delivered to the Au spots. (c) First rinse step: a washing buffer (clear liquid) is injected to rinse the Au spots. (d) Horizontal reagent injection step: a reagent (black liquid) is injected into the flow channels then delivered to the Au spots. (e) Second rinse step: a washing buffer (clear liquid) is injected to rinse the Au spots. (f) The channels in the microfluidic device are clear after the second rinse. Reprinted with permission from ref. 168. Copyright (2008) Royal Society of Chemistry.

Article Snippet: [ 166 ] Lee et al. demonstrated the simultaneous detection of multiple hybridization reactions based on SPR imaging integrated with microfluidic systems that include two perpendicular sets of fluidic channels.

Techniques: Imaging, Injection