Journal: Frontiers in Genetics

Article Title: An investigation into anti-proliferative effects of microRNAs encoded by the miR-106a-363 cluster on human carcinoma cells and keratinocytes using microarray profiling of miRNA transcriptomes

doi: 10.3389/fgene.2014.00246

Figure Lengend Snippet: MicroRNAs differentially expressed in cultured human squamous carcinoma (E10) cells transfected with scrambled control, miR-19a -, miR-20b -, miR-92a, or miR-363-5p- mimic . The microRNA transcriptome of E10 cells transfected with miR-19a -, miR-20b -, mir92a -, or miR-363-5p mimic were individually compared against transcriptome from scrambled control transfectants. ANOVA ( P ≤ 0.05) was used for the isolation of the 53 mirNA differentially expressed in transfectants with miR-19a mimic (A) , 43 miRNAs in miR-20b transfectants (B) , 61 miRNAs in miR-92a transfectants (C) and 29 miRNAs in transfectants with miR-363-5p mimic (D) . Each cluster was separately subjected to hierarchical clustering yielding the heat diagrams shown in the figure. The heat-map was computed using Spotfire DecisionSite for Microarray analysis as described in Materials and Methods. The color scale indicates fluorescence intensities. The results were obtained by microarray analysis of mirNA transcriptomes using four separate transfections for each mirNA mimic and four scrambled control transfections as described in Materials and Methods.

Article Snippet: The statistical analysis was carried out using SPOTFIRE v. 9.0 DecisionSite for Microarray Analysis software (TIBCO Software, Palo Alto, CA, USA).

Techniques: Cell Culture, Transfection, Isolation, Microarray, Fluorescence