Journal:

Article Title: A Photoresist with Low Fluorescence for Bioanalytical Applications

doi: 10.1021/ac071528q

Figure Lengend Snippet: Process Parameters for 1002F Photoresist

Article Snippet: Absorbance of 1002F Photoresist Many bioapplications using photoresists as a structural material employ absorbance measurements for analyte detection.

Techniques:

Journal:

Article Title: A Photoresist with Low Fluorescence for Bioanalytical Applications

doi: 10.1021/ac071528q

Figure Lengend Snippet: Fluorescence of blocks of SU-8 and 1002F resin and photoresist. The blocks were excited at 485 nm with a ±2 nm band pass and the fluorescence measured from 490 to 650 nm with a ±2 nm band pass. The upper solid and dashed lines are SU-8 photoresist and SU-8 resin, respectively. The dotted and dash-dotted line are 1002F photoresist and 1002F resin, respectively. The lower solid line is PDMS. For technical reasons, the photoresist blocks were not treated with UV exposure and post-exposure baking.

Article Snippet: Absorbance of 1002F Photoresist Many bioapplications using photoresists as a structural material employ absorbance measurements for analyte detection.

Techniques: Fluorescence

Journal:

Article Title: A Photoresist with Low Fluorescence for Bioanalytical Applications

doi: 10.1021/ac071528q

Figure Lengend Snippet: Images of 1002F pallets. (A) Transmitted light microscopy of an array of pallets. Each pallet was square in cross section with a 50 μm side and 75 μm height. The distance between each pallet was 50 μm. (B) SEM of the pallets shown in (A). (C) SEM of an array of pallets. Each pallet was circular in cross-section with a 25 μm diameter and 100 μm height. (D) Transmitted light microscopy of four pallet arrays fabricated on a single glass slide. The pallets on the left side of the image were fabricated from a mask with circular openings of diameter 10 μm (top array) or 5 μm (bottom array). The pallets on the right side of the image were square openings with a side of 10 μm (top array) or 5 μm (bottom array). The spacing between the 5-μm and 10-μm pallets was 5 μm and 10 μm, respectively.

Article Snippet: Absorbance of 1002F Photoresist Many bioapplications using photoresists as a structural material employ absorbance measurements for analyte detection.

Techniques: Light Microscopy

Journal:

Article Title: A Photoresist with Low Fluorescence for Bioanalytical Applications

doi: 10.1021/ac071528q

Figure Lengend Snippet: Spectral properties of SU-8 and 1002F photoresists after UV exposure and post-exposure bake. (A) Fluorescence of SU-8 (solid squares) and 1002F (open circles) of lines (100 μm wide) of varying heights. The fluorescence was collected with a filter set designed for fluorescein (excitation filter 450-490 nm, dichroic 500 nm long pass, emission 520 nm long pass). (B,C) Same as (A) but the fluorescence was collected using a filter set for tetramethyl rhodamine (excitation filter 528-553 nm dichroic 565 nm long pass, emission 590-650 nm) (B) or Cy5 (excitation filter 590-650 nm, dichroic 660 nm long pass, emission 665-740 nm) (C). The error bars represent the standard deviation of 5 measurements. (D) The absorbance of glass (dotted line), 1002F photoresist (dashed line), and SU-8 photoresist (solid line) is shown at varying wavelengths.

Article Snippet: Absorbance of 1002F Photoresist Many bioapplications using photoresists as a structural material employ absorbance measurements for analyte detection.

Techniques: Fluorescence, Standard Deviation

Journal:

Article Title: A Photoresist with Low Fluorescence for Bioanalytical Applications

doi: 10.1021/ac071528q

Figure Lengend Snippet: Measurement of metabolism by XTT assay of cells grown on uncoated photoresists. RBL (A) or A172 (B) cells were cultured on glass (solid triangles), 1002F photoresists (open circles), or SU-8 photoresist (solid squares) for varying times. Shown on the “y” axis is the absorbance of the orange formazon product produced by metabolically active cells. The error bars represent the standard deviation of 4 measurements.

Article Snippet: Absorbance of 1002F Photoresist Many bioapplications using photoresists as a structural material employ absorbance measurements for analyte detection.

Techniques: XTT Assay, Cell Culture, Produced, Metabolic Labelling, Standard Deviation

Journal:

Article Title: A Photoresist with Low Fluorescence for Bioanalytical Applications

doi: 10.1021/ac071528q

Figure Lengend Snippet: Properties of SU-8 and 1002F pallets fabricated in arrays. (A,B) Transmitted light images of HeLa cells grown on 1002F (A) or SU-8 (B) pallets on arrays with virtual walls. (C,D) Fluorescence images of HeLa cells loaded with Oregon Green on 1002F (C) or SU-8 (D) pallet arrays. (E) Measurement of the threshold energy for pallet release. Shown on the “y” axis is the fraction of pallets released at each energy tested. Ten pallets were released for each data point. The energy of each pulse sent from the laser was measured. The “x” coordinate represents the average energy at a given laser setting and the error bars represent the standard deviation. The dimensions of the pallets in (A)-(E) were 50 μm (side) and 100 μm (height). The distance between each pallet was 50 μm.

Article Snippet: Absorbance of 1002F Photoresist Many bioapplications using photoresists as a structural material employ absorbance measurements for analyte detection.

Techniques: Fluorescence, Standard Deviation