Journal: Molecular and cellular endocrinology

Article Title: Role of cAMP/PKA pathway and T-type calcium channels in the mechanism of action of serotonin in human adrenocortical cells

doi: 10.1016/j.mce.2016.10.008

Figure Lengend Snippet: Coupling of 5-HT receptors to T-type Ca2+ currents in human cultured adrenocortical cells. (A) Superimposed Ca2+ currents elicited by increasing depolarizing pulses (from −60 mV to −30 mV) from a holding potential of −80 mV (upper panel). Current-voltage relationships of the peak Ca2+ currents (lower panel). (B) Superimposed Ca2+ currents elicited by depolarizing pulses from −80 mV to −50 mV and −40 mV recorded in the absence (control) or presence of 5-HT (10−5 M). (C) Effects of graded concentrations of 5-HT on cortisol secretion by adrenocortical cells cultured in the absence or presence of the T-type Ca2+ channel blockers mibefradil (10−6 M; ■) and NiCl2 (8 × 10−5 M; ●). (D) Effects of the L-type channel blocker nifedipine (10−5 M), N-type channel blocker ω-conotoxin (10−6 M) and P-type channel blocker sFTX-3.3 (10−6 M) on basal and 5-HT-induced cortisol production. BL, Basal level. *, p < 0.05; ***, p < 0.001 versus basal production in the absence of test substance. ###, p < 0.001 versus production in the presence of 5-HT.

Article Snippet: Serotonin, dibutyryladenosine 3′,5′-cyclic monophosphate (dbcAMP), 1-methyl-3-isobutylxanthine (IBMX), chelerythrine, H-89, GR113808, zacopride, EGTA, nifedipine, mibefradil, NiCl 2 were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). ω-conotoxin and sFTX-3.3 were obtained from Alomone Labs (Jerusalem, Israel).

Techniques: Cell Culture

Journal:

Article Title: Functional properties and pharmacological inhibition of ASIC channels in the human SJ-RH30 skeletal muscle cell line

doi: 10.1113/jphysiol.2004.075069

Figure Lengend Snippet: In each case, a representative experiment is shown in the left panel. Summary graphs on the right confirm the significant reduction of current amplitudes by amiloride and Psalmopoeus venom. A, 30 mm amiloride inhibits ASIC responses by 84.4 ± 1.2%; they recover to 93.3 ± 3.4% of control upon wash-out. B, in response to Psalmopeus venom (1 : 1000), currents are reduced by 90.8 ± 3.0% and return to 80.9 ± 1.4% after wash-out. By contrast, APETx2 had no significant effect on acid-evoked responses. C, at a concentration of 1 μm, the toxin reduced currents to 95.0 ± 3.8% of control (91.4 ± 5.4% of control on wash-out response).

Article Snippet: Amiloride and lactic acid were obtained from Sigma; Psalmopoeus cambridgei venom was obtained from Spider Pharm Inc. (Yarnell, AZ, USA) and APETx2 was custom synthesized.

Techniques: Control, Concentration Assay