speedvac Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Eppendorf AG speedvac
    Speedvac, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 694 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/Eppendorf AG
    Average 93 stars, based on 694 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Labconco speedvac
    Speedvac, supplied by Labconco, used in various techniques. Bioz Stars score: 93/100, based on 386 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/Labconco
    Average 93 stars, based on 386 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Martin Christ Gefriertrocknungsanlagen speedvac
    Speedvac, supplied by Martin Christ Gefriertrocknungsanlagen, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/Martin Christ Gefriertrocknungsanlagen
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Savant Instruments speedvac
    Speedvac, supplied by Savant Instruments, used in various techniques. Bioz Stars score: 92/100, based on 435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/Savant Instruments
    Average 92 stars, based on 435 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Thermo Fisher speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 4701 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/Thermo Fisher
    Average 94 stars, based on 4701 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    SP Industries speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac, supplied by SP Industries, used in various techniques. Bioz Stars score: 92/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/SP Industries
    Average 92 stars, based on 113 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    GMI Inc speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac, supplied by GMI Inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/GMI Inc
    Average 93 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Millipore speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/Millipore
    Average 92 stars, based on 603 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    UniEquip Laborger speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac, supplied by UniEquip Laborger, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/UniEquip Laborger
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Fisher Scientific speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/Fisher Scientific
    Average 93 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Eyela Co speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac, supplied by Eyela Co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/Eyela Co
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Tokyo Rikakikai speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac, supplied by Tokyo Rikakikai, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac/product/Tokyo Rikakikai
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    speedvac - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    SP Industries a speedvac concentrator
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    A Speedvac Concentrator, supplied by SP Industries, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a speedvac concentrator/product/SP Industries
    Average 92 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    a speedvac concentrator - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    90
    Biodirect savant speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Savant Speedvac, supplied by Biodirect, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/savant speedvac/product/Biodirect
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    savant speedvac - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

    88
    Thermo Fisher spd speedvac
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Spd Speedvac, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spd speedvac/product/Thermo Fisher
    Average 88 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    spd speedvac - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    94
    Labconco speedvac concentrator
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac Concentrator, supplied by Labconco, used in various techniques. Bioz Stars score: 94/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac concentrator/product/Labconco
    Average 94 stars, based on 264 article reviews
    Price from $9.99 to $1999.99
    speedvac concentrator - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    93
    Savant Instruments speedvac concentrator
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac Concentrator, supplied by Savant Instruments, used in various techniques. Bioz Stars score: 93/100, based on 220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac concentrator/product/Savant Instruments
    Average 93 stars, based on 220 article reviews
    Price from $9.99 to $1999.99
    speedvac concentrator - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    88
    Thermo Fisher speedvac evaporator
    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a <t>SpeedVac</t> (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.
    Speedvac Evaporator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/speedvac evaporator/product/Thermo Fisher
    Average 88 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    speedvac evaporator - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a SpeedVac (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.

    Journal: mSystems

    Article Title: Clostridium difficile Modulates the Gut Microbiota by Inducing the Production of Indole, an Interkingdom Signaling and Antimicrobial Molecule

    doi: 10.1128/mSystems.00346-18

    Figure Lengend Snippet: Comparative analysis of metabolites present in CDI-positive and CDI-negative stools by preparative high-performance liquid chromatography using a C 18 column. Stools (1 g each) from 20 diarrhea patients infected with C. difficile and 20 diarrhea patients without C. difficile were pooled, suspended in PBS, and acetate precipitated. The precipitated material was resuspended in PBS and filtered through a centrifugal filter with a 3-kDa-cutoff membrane, and the filtrate was concentrated in a SpeedVac (Thermo Fisher Scientific, Waltham, MA). The concentrated filtrate was injected into a preparative Econosil C 18 column (250 mm by 10 mm; Alltech) and purified using a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD). Each purification run was performed by injecting 1 ml of the sample and washing with buffer A (97.5% [vol/vol] acetic acid-H 2 O; pH 3.8), followed by gradient elution with buffer B (80%:20% [vol/vol] acetonitrile/H 2 O). The red arrow indicates the unique indole peak. *, peak contained the autoinducing quorum signaling peptide (TI signal) associated with C. difficile toxin regulation. A representative chromatogram from multiple runs is shown. mAU, milli-arbitrary units.

    Article Snippet: The filtrate was concentrated in a SpeedVac (Thermo Fisher Scientific, Waltham, MA) and purified by preparative high-performance liquid chromatography (HPLC) using an Econosil C18 column (250 mm by 10 mm; Alltech) with a Shimadzu Prominence HPLC system (Shimadzu Scientific Instruments, Columbia, MD).

    Techniques: High Performance Liquid Chromatography, Infection, Injection, Purification