Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: The summary results inflammation cytokines level by ELISA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis reduced the expression of IgE in the serum of allergic asthma mice. ( A ) ELISA was used to measure the total IgE content (ng/ml) in the serum of mice. ( B ) ELISA was used to detect the content of OVA-specific IgE (ng/ml) in the serum of mice. All experiments were repeated in triplicate to average (n=10). ## p <0.001 vs. control; ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Bifidobacterium infantis Relieves Allergic Asthma in Mice by Regulating Th1/Th2

doi: 10.12659/MSM.920583

Figure Lengend Snippet: B. infantis regulated the balance of Th1/Th2-related cytokines in BALF and lung tissues. ( A–E ) ELISA was used to detect the contents of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in BALF. ( F–J ) QRT-PCR was used to detect the expression of IFN-γ, IL-2, IL-4, IL-5, and IL-13 in lung tissues. β-actin served as a reference gene. All experiments were repeated in triplicate to average (n=10). # p <0.05, ## p <0.001 vs. control; * p <0.05, ** p <0.001 vs. OVA.

Article Snippet: Total IgE and OVA-specific IgE in the serum of each group of mice were measured using a Mouse IgE ELISA Kit (CSB-E07983m, Cusabio, China) and a Mouse OVA sIgE ELISA Kit (CSB-E08914m, Cusabio, China).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Control

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Promotor Hypomethylation Mediated Upregulation of VCAN Targets Twist1 to Promote EndMT in Hypoxia‐Induced Pulmonary Hypertension

doi: 10.1161/JAHA.124.036969

Figure Lengend Snippet: A , RT‐qPCR analysis of the mRNA level of VCAN from HPAECs (n=3). ** P <0.01. ( B ) CpG sites of the promoter of VCAN gene. ( C and D ) DNA methylation levels of the VCAN promotor region in HPAECs and corresponding densitometric quantification as detected by MSP assay (n=3). *** P <0.001. ( E and F ) The protein levels and densitometric quantification of DNMT3a and DNMT3b were determined by Western blot analysis after the hypoxia treatment (n=3). * P <0.05, *** P <0.001. ( G–J ) Representative images of immunohistochemical staining confirmed that DNMT3a and DNMT3b were significantly decreased in HPH mice compared with the normal control group (n=3). Positive staining was indicated by a brown color and pointed out with black arrow. ×400, scale bar: 50 μm. *** P <0.001. ( K and L ) The protein levels and densitometric quantification of DNMTs and VCAN levels in SGI‐1027‐treated HPAECs (n=3). ** P <0.01, *** P <0.001. ( M and N ) DNA methylation levels of the VCAN promotor region in 5 μM SGI‐1027‐treated HPAECs and corresponding densitometric quantification as detected by MSP assay (n=3). *** P <0.001. An unpaired 2‐tailed Student t test was performed for comparisons between 2 groups. CpG indicates Cytosine‐phosphate‐Guanine; DNMT, DNA methyltransferase; HPAECs, human pulmonary artery endothelial cells; HPH, hypoxia‐induced pulmonary hypertension; MSP, methylation‐specific polymerase chain reaction; RT‐qPCR, reverse transcription‐quantitative polymerase chain reaction; and VCAN, versican.

Article Snippet: The modified DNA was then subjected to real‐time PCR using the methylation‐specific PCR kit (Tiangen, China) to specifically amplify the bisulfite‐converted DNA of the promoter region of versican.

Techniques: Quantitative RT-PCR, DNA Methylation Assay, MSP Assay, Western Blot, Immunohistochemical staining, Staining, Control, Methylation, Polymerase Chain Reaction, Reverse Transcription, Real-time Polymerase Chain Reaction