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  • 99
    Millipore spe
    Data and flow illustrations representing the coupling of <t>SPE</t> and <t>PCR</t> sample preparation steps on the MGA device using elastomeric valves and flow control preset by channel design. ( a ) Flow control between SPE and PCR was accomplished by using differential
    Spe, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spe - by Bioz Stars, 2020-04
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    84
    Thermo Fisher spe plates
    Data and flow illustrations representing the coupling of <t>SPE</t> and <t>PCR</t> sample preparation steps on the MGA device using elastomeric valves and flow control preset by channel design. ( a ) Flow control between SPE and PCR was accomplished by using differential
    Spe Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    TaKaRa spe i
    PCR-RFLP patterns of COI region from JBS samples (D1–D11) digested with <t>Spe</t> I and Bst EII, DNA marker (M) in bp were indicated.
    Spe I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 271 article reviews
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    94
    Millipore spe tube
    PCR-RFLP patterns of COI region from JBS samples (D1–D11) digested with <t>Spe</t> I and Bst EII, DNA marker (M) in bp were indicated.
    Spe Tube, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Metrohm spes
    PCR-RFLP patterns of COI region from JBS samples (D1–D11) digested with <t>Spe</t> I and Bst EII, DNA marker (M) in bp were indicated.
    Spes, supplied by Metrohm, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spes - by Bioz Stars, 2020-04
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    99
    Millipore supelmip spe fqs
    PCR-RFLP patterns of COI region from JBS samples (D1–D11) digested with <t>Spe</t> I and Bst EII, DNA marker (M) in bp were indicated.
    Supelmip Spe Fqs, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore spe cartridge
    PCR-RFLP patterns of COI region from JBS samples (D1–D11) digested with <t>Spe</t> I and Bst EII, DNA marker (M) in bp were indicated.
    Spe Cartridge, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher spe
    PCR-RFLP patterns of COI region from JBS samples (D1–D11) digested with <t>Spe</t> I and Bst EII, DNA marker (M) in bp were indicated.
    Spe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Applied Separations Inc dio spe ed spe cartridges
    PCR-RFLP patterns of COI region from JBS samples (D1–D11) digested with <t>Spe</t> I and Bst EII, DNA marker (M) in bp were indicated.
    Dio Spe Ed Spe Cartridges, supplied by Applied Separations Inc, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Toxin Technology Inc spe a
    Histamine release and degranulation from HMC-1 cells stimulated with rSPE B/SCP. HMC-1 cells were incubated at 2 × 10 6 cells/ml in <t>Tyrode's</t> solution for 20 min at 37°C with rSPE B/SCP, SPE A, and LPS. After incubation, histamine in the supernatant was measured by enzyme-linked immunosorbent assay. (A) Data are expressed as a percentage of release of histamine in comparison to control. As a control, the HMC-1 cells were allowed to freeze and thaw for three cycles to release the remaining histamine from the cells. (B) Degranulation pattern from HMC-1 cells stimulated with rSPE B/SCP. Panel 1, control (nonstimulated HMC-1 cells); panel 2, rSPE B/SCP-stimulated HMC-1 cells.
    Spe A, supplied by Toxin Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore spe 7
    Characterization of Pak1 −/− bone marrow–derived mast cells . (A) Bone marrow–derived mast cell (BMMC) receptor expression. BMMCs were maintained in culture for 5 weeks, and expression of c-kit and FcϵRI was measured by incubation with antimouse CD 117 (c-kit) PE-conjugated antibody, and anti-DNP monoclonal antibody IgE clone <t>SPE-7</t> followed by incubation with FITC-conjugated anti–mouse IgE secondary antibody. Double-positive cells (top right quadrant) are mature mast cells, expressing both c-kit and FcϵRI. Data shown are representative of 6 independent lines from each genotype. (Mean WT = 96.1 + 2.3 SEM % vs Pak1 −/− = 95.3 + 1.7 SEM % double-positive cells, n = 6.) (B) IgE-mediated Pak1 activation in BMMCs (representative of 3 independent experiments). IgE-primed BMMCs were stimulated with antigen (DNP) for the indicated times, lysates were precipitated with anti-Pak1 antibody, and Pak1 activity was assayed. (C) Pak1 activation of pS298-MEK1 in Wt and Pak1 −/− BMMCs. IgE-primed BMMCs were stimulated with antigen (DNP) for the indicated times. Cell lysates (“Western blotting”) were subjected to immunoblotting with anti–phospho-S298 MEK1 (top blot) or anti–total MEK1/2 (bottom blot). (D) Effect of IPA-3 treatment in Wt BMMCs. The length of activation and the addition of inhibitor are indicated.
    Spe 7, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Phenomenex spe cartridge
    Outline of the high resolution nano-LC-MS/MS method for the analysis of <t>NNAL,</t> NNAL O-glucuronide and NNAL N-glucuronide in smoker’s urine. a <t>SPE</t> = solid phase extraction.
    Spe Cartridge, supplied by Phenomenex, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Waters Corporation spe cartridge
    Comparison of methods used to extract marine toxins tetrodotoxin (TTX), saxitoxin (STX), and neosaxitoxin (NEO): A) All analytes extracted from urine matrix using Oasis <t>MCX</t> online <t>SPE</t> cartridge and Halo Penta-HILIC LC column. B) All analytes extracted from urine matrix using Oasis WCX online SPE cartridge and Atlantis Silica HILIC LC column.
    Spe Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    BIOTAGE spe column
    Comparison of methods used to extract marine toxins tetrodotoxin (TTX), saxitoxin (STX), and neosaxitoxin (NEO): A) All analytes extracted from urine matrix using Oasis <t>MCX</t> online <t>SPE</t> cartridge and Halo Penta-HILIC LC column. B) All analytes extracted from urine matrix using Oasis WCX online SPE cartridge and Atlantis Silica HILIC LC column.
    Spe Column, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher spe column
    Comparison of methods used to extract marine toxins tetrodotoxin (TTX), saxitoxin (STX), and neosaxitoxin (NEO): A) All analytes extracted from urine matrix using Oasis <t>MCX</t> online <t>SPE</t> cartridge and Halo Penta-HILIC LC column. B) All analytes extracted from urine matrix using Oasis WCX online SPE cartridge and Atlantis Silica HILIC LC column.
    Spe Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Phenomenex spe columns
    Comparison of methods used to extract marine toxins tetrodotoxin (TTX), saxitoxin (STX), and neosaxitoxin (NEO): A) All analytes extracted from urine matrix using Oasis <t>MCX</t> online <t>SPE</t> cartridge and Halo Penta-HILIC LC column. B) All analytes extracted from urine matrix using Oasis WCX online SPE cartridge and Atlantis Silica HILIC LC column.
    Spe Columns, supplied by Phenomenex, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Waters Corporation spe columns
    Evaluation of 2DLC configuration. NAc-linker-DSEA, spiked into a dilute <t>trastuzumab</t> sample, was successfully transferred from ( A ) the <t>SPE</t> column (1 st dimension) to the ( B ) RP column (2 nd dimension) using the 2DLC configuration illustrated in Figure 4 A as proof-of-principle.
    Spe Columns, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher spe i
    PFGE fingerprints of 45 E. aerogenes isolates (1995) after digestion with the <t>Xba</t> I restriction enzyme (A) and the <t>Spe</t> I restriction enzyme (B). Lane FH54 to lane BG64, profile IA and profile IB (broadly clone I); lane FH51 to lane FH90, profiles IIA, IIB, and IIC (broadly, clone II); lane FH49 to lane FH77, unrelated strains (nonclonal); lanes M, molecular size markers (PFGE marker I, λ ladder).
    Spe I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1336 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Waters Corporation spe plates
    PFGE fingerprints of 45 E. aerogenes isolates (1995) after digestion with the <t>Xba</t> I restriction enzyme (A) and the <t>Spe</t> I restriction enzyme (B). Lane FH54 to lane BG64, profile IA and profile IB (broadly clone I); lane FH51 to lane FH90, profiles IIA, IIB, and IIC (broadly, clone II); lane FH49 to lane FH77, unrelated strains (nonclonal); lanes M, molecular size markers (PFGE marker I, λ ladder).
    Spe Plates, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Supelco spe tube
    PFGE fingerprints of 45 E. aerogenes isolates (1995) after digestion with the <t>Xba</t> I restriction enzyme (A) and the <t>Spe</t> I restriction enzyme (B). Lane FH54 to lane BG64, profile IA and profile IB (broadly clone I); lane FH51 to lane FH90, profiles IIA, IIB, and IIC (broadly, clone II); lane FH49 to lane FH77, unrelated strains (nonclonal); lanes M, molecular size markers (PFGE marker I, λ ladder).
    Spe Tube, supplied by Supelco, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Supelco lc18 spe
    PFGE fingerprints of 45 E. aerogenes isolates (1995) after digestion with the <t>Xba</t> I restriction enzyme (A) and the <t>Spe</t> I restriction enzyme (B). Lane FH54 to lane BG64, profile IA and profile IB (broadly clone I); lane FH51 to lane FH90, profiles IIA, IIB, and IIC (broadly, clone II); lane FH49 to lane FH77, unrelated strains (nonclonal); lanes M, molecular size markers (PFGE marker I, λ ladder).
    Lc18 Spe, supplied by Supelco, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    MACHEREY NAGEL spe cartridge
    PFGE fingerprints of 45 E. aerogenes isolates (1995) after digestion with the <t>Xba</t> I restriction enzyme (A) and the <t>Spe</t> I restriction enzyme (B). Lane FH54 to lane BG64, profile IA and profile IB (broadly clone I); lane FH51 to lane FH90, profiles IIA, IIB, and IIC (broadly, clone II); lane FH49 to lane FH77, unrelated strains (nonclonal); lanes M, molecular size markers (PFGE marker I, λ ladder).
    Spe Cartridge, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 95/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    MACHEREY NAGEL spe column
    PFGE fingerprints of 45 E. aerogenes isolates (1995) after digestion with the <t>Xba</t> I restriction enzyme (A) and the <t>Spe</t> I restriction enzyme (B). Lane FH54 to lane BG64, profile IA and profile IB (broadly clone I); lane FH51 to lane FH90, profiles IIA, IIB, and IIC (broadly, clone II); lane FH49 to lane FH77, unrelated strains (nonclonal); lanes M, molecular size markers (PFGE marker I, λ ladder).
    Spe Column, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Avantor spe columns
    PFGE fingerprints of 45 E. aerogenes isolates (1995) after digestion with the <t>Xba</t> I restriction enzyme (A) and the <t>Spe</t> I restriction enzyme (B). Lane FH54 to lane BG64, profile IA and profile IB (broadly clone I); lane FH51 to lane FH90, profiles IIA, IIB, and IIC (broadly, clone II); lane FH49 to lane FH77, unrelated strains (nonclonal); lanes M, molecular size markers (PFGE marker I, λ ladder).
    Spe Columns, supplied by Avantor, used in various techniques. Bioz Stars score: 87/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    spe i  (Roche)
    95
    Roche spe i
    PFGE patterns of genomic DNAs of B. lactis strains after <t>Spe</t> I digestion. Lanes: 1, B. lactis NCC 383; 2, B. lactis NCC 363; 3, B. lactis DSM 10140; 4, B. lactis NCC 402; 5, B. lactis DSM 10140; 6, B. lactis NCC 239; 7, B. lactis NCC 387; 8, B. lactis NCC 311; 9, B. lactis DSM 10140; M, λ DNA ladder (Bio-Rad).
    Spe I, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    BIOTAGE additional spe
    PFGE patterns of genomic DNAs of B. lactis strains after <t>Spe</t> I digestion. Lanes: 1, B. lactis NCC 383; 2, B. lactis NCC 363; 3, B. lactis DSM 10140; 4, B. lactis NCC 402; 5, B. lactis DSM 10140; 6, B. lactis NCC 239; 7, B. lactis NCC 387; 8, B. lactis NCC 311; 9, B. lactis DSM 10140; M, λ DNA ladder (Bio-Rad).
    Additional Spe, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Hichrom Limited spe carbo
    PFGE patterns of genomic DNAs of B. lactis strains after <t>Spe</t> I digestion. Lanes: 1, B. lactis NCC 383; 2, B. lactis NCC 363; 3, B. lactis DSM 10140; 4, B. lactis NCC 402; 5, B. lactis DSM 10140; 6, B. lactis NCC 239; 7, B. lactis NCC 387; 8, B. lactis NCC 311; 9, B. lactis DSM 10140; M, λ DNA ladder (Bio-Rad).
    Spe Carbo, supplied by Hichrom Limited, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Merck & Co spe cartridges
    Electropherograms obtained during the electrophoretic separation of human urine samples containing eight steroid hormones after <t>SPE</t> using C 18 cartridges and elution of steroids with: methanol ( A ), and dichloromethane ( B ), next SPE using <t>HLB</t> cartridges and elution of steroids with methanol ( C ), dichloromethane ( D ). Conditions: UV detection at 254 nm, unmodified silica capillary (57 cm × 50 μm I.D.), temp. 22 °C, running buffer composed of (10:90, v/v) methanol and mixture of 10 mM Na 2 B 4 O 7 and 50 mM SDS.
    Spe Cartridges, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    3M Co spe column
    Electropherograms obtained during the electrophoretic separation of human urine samples containing eight steroid hormones after <t>SPE</t> using C 18 cartridges and elution of steroids with: methanol ( A ), and dichloromethane ( B ), next SPE using <t>HLB</t> cartridges and elution of steroids with methanol ( C ), dichloromethane ( D ). Conditions: UV detection at 254 nm, unmodified silica capillary (57 cm × 50 μm I.D.), temp. 22 °C, running buffer composed of (10:90, v/v) methanol and mixture of 10 mM Na 2 B 4 O 7 and 50 mM SDS.
    Spe Column, supplied by 3M Co, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies dispersive spe
    Electropherograms obtained during the electrophoretic separation of human urine samples containing eight steroid hormones after <t>SPE</t> using C 18 cartridges and elution of steroids with: methanol ( A ), and dichloromethane ( B ), next SPE using <t>HLB</t> cartridges and elution of steroids with methanol ( C ), dichloromethane ( D ). Conditions: UV detection at 254 nm, unmodified silica capillary (57 cm × 50 μm I.D.), temp. 22 °C, running buffer composed of (10:90, v/v) methanol and mixture of 10 mM Na 2 B 4 O 7 and 50 mM SDS.
    Dispersive Spe, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Data and flow illustrations representing the coupling of SPE and PCR sample preparation steps on the MGA device using elastomeric valves and flow control preset by channel design. ( a ) Flow control between SPE and PCR was accomplished by using differential

    Journal:

    Article Title: A fully integrated microfluidic genetic analysis system with sample-in-answer-out capability

    doi: 10.1073/pnas.0604663103

    Figure Lengend Snippet: Data and flow illustrations representing the coupling of SPE and PCR sample preparation steps on the MGA device using elastomeric valves and flow control preset by channel design. ( a ) Flow control between SPE and PCR was accomplished by using differential

    Article Snippet: The SPE and PCR domains, along with the syringe used to deliver master mix, were silanized by using Sigmacote (Sigma-Aldrich, St. Louis, MO).

    Techniques: Flow Cytometry, Polymerase Chain Reaction, Sample Prep

    PFGE banding patterns after Spe I digestion. Lanes 1 to 3, VIM-1-producing P. putida isolates from the general ICU (lane 1, isolate VA-304/99; lane 2, isolate VA-523/99; lane 3, isolate VA-420/00); lane 4, multidrug-resistant P. putida isolate from the nephrology department; lanes 5 and 6, two antibiotic-susceptible P. putida isolates obtained from the general ICU at the same hospital in 2000; lane L, λ phage DNA concatemers as a size marker.

    Journal: Journal of Clinical Microbiology

    Article Title: Nosocomial Infections Caused by Multidrug-Resistant Isolates of Pseudomonas putida Producing VIM-1 Metallo-?-Lactamase

    doi: 10.1128/JCM.40.11.4051-4055.2002

    Figure Lengend Snippet: PFGE banding patterns after Spe I digestion. Lanes 1 to 3, VIM-1-producing P. putida isolates from the general ICU (lane 1, isolate VA-304/99; lane 2, isolate VA-523/99; lane 3, isolate VA-420/00); lane 4, multidrug-resistant P. putida isolate from the nephrology department; lanes 5 and 6, two antibiotic-susceptible P. putida isolates obtained from the general ICU at the same hospital in 2000; lane L, λ phage DNA concatemers as a size marker.

    Article Snippet: Restrictions were carried out overnight at 37°C with 10 U of Spe I (Sigma, Milan, Italy).

    Techniques: Marker

    Ability of antibodies against either wild-type SPE A or mutants to neutralize the superantigenicity of SPE A. Dilutions of antibodies were mixed with wild-type SPE A (1 μg/well) 1 h prior to addition of rabbit splenocytes. Cultures were incubated for 3 days at 37°C in a 7% CO 2 incubator, and then 1 μCi of [ 3 H]thymidine was added per well. DNA was harvested after an additional day of incubation. Error bars indicate standard deviations.

    Journal: Infection and Immunity

    Article Title: Toxoids of Streptococcal Pyrogenic Exotoxin A Are Protective in Rabbit Models of Streptococcal Toxic Shock Syndrome

    doi:

    Figure Lengend Snippet: Ability of antibodies against either wild-type SPE A or mutants to neutralize the superantigenicity of SPE A. Dilutions of antibodies were mixed with wild-type SPE A (1 μg/well) 1 h prior to addition of rabbit splenocytes. Cultures were incubated for 3 days at 37°C in a 7% CO 2 incubator, and then 1 μCi of [ 3 H]thymidine was added per well. DNA was harvested after an additional day of incubation. Error bars indicate standard deviations.

    Article Snippet: Bound anti-SPE A was detected by goat anti-rabbit immunoglobulin G conjugated with peroxidase (Sigma).

    Techniques: Incubation

    Minichromosome DNA is cut at only one site by SpeI or SwaI, which have seven and two cutting sites, respectively. ( A ) Circular minichromosome DNA showing SpeI, SwaI and PacI sites; SpeI fragment lengths are not shown for clarity. ( B ) Minichromosome DNA from permeabilized cells incubated with SpeI or ( C ) with SwaI, and deproteinized. Lane DNA, SwaI fragments produced in deproteinized cells. ( D ) The SwaI cleavage site mapped by gel hybridization. DNA linearized by SwaI (200 U/ml, 2 h) was isolated from a PFGE gel, cut by PacI (100 U/ml, 18 h), and the products were separated by PFGE. Lanes M, oligomers with HindIII fragments of λ DNA. The four fragments produced (∼140, 100, 72 and 29 kb) represent a mixture of the pairs produced after SwaI had cut at only one of its two sites in different minichromosomes. ( E ) The SwaI cleavage site mapped by fibre-FISH. The positions of the SwaI sites and hybridization probes (green) are shown above; the biotin-labelled probes were detected with anti-biotin antibodies (green) and BrdU-labelled DNA with anti-BrdU antibodies (red). Images show representative linear molecules from the two classes observed, which had been cut by SwaI (shown by the extremities of the molecule) at either the left (upper panel) or the right (lower panel) site on the map. The probe positions were aligned approximately considering the variable stretching during combing ( 22 , 23 ). Below, linear molecules cleaved at the single PacI site for comparison.

    Journal: Nucleic Acids Research

    Article Title: DNA of a circular minichromosome linearized by restriction enzymes or other reagents is resistant to further cleavage: an influence of chromatin topology on the accessibility of DNA

    doi: 10.1093/nar/gks723

    Figure Lengend Snippet: Minichromosome DNA is cut at only one site by SpeI or SwaI, which have seven and two cutting sites, respectively. ( A ) Circular minichromosome DNA showing SpeI, SwaI and PacI sites; SpeI fragment lengths are not shown for clarity. ( B ) Minichromosome DNA from permeabilized cells incubated with SpeI or ( C ) with SwaI, and deproteinized. Lane DNA, SwaI fragments produced in deproteinized cells. ( D ) The SwaI cleavage site mapped by gel hybridization. DNA linearized by SwaI (200 U/ml, 2 h) was isolated from a PFGE gel, cut by PacI (100 U/ml, 18 h), and the products were separated by PFGE. Lanes M, oligomers with HindIII fragments of λ DNA. The four fragments produced (∼140, 100, 72 and 29 kb) represent a mixture of the pairs produced after SwaI had cut at only one of its two sites in different minichromosomes. ( E ) The SwaI cleavage site mapped by fibre-FISH. The positions of the SwaI sites and hybridization probes (green) are shown above; the biotin-labelled probes were detected with anti-biotin antibodies (green) and BrdU-labelled DNA with anti-BrdU antibodies (red). Images show representative linear molecules from the two classes observed, which had been cut by SwaI (shown by the extremities of the molecule) at either the left (upper panel) or the right (lower panel) site on the map. The probe positions were aligned approximately considering the variable stretching during combing ( 22 , 23 ). Below, linear molecules cleaved at the single PacI site for comparison.

    Article Snippet: Restriction fragments of this DNA cut with SpeI or SwaI (100 U/ml) were separated by PFGE in 1% LMP agarose at 190 V/cm for 7 or 20 h and switch time ramped linearly from 0.4 to 6 or 0.3 to 3 s, respectively, excised from gels and purified on Ultrafree columns (Millipore).

    Techniques: Incubation, Produced, Hybridization, Isolation, Fluorescence In Situ Hybridization

    Sites of breakage of minichromosome DNA in γ-irradiated cells. ( A ) SpeI and SwaI EBV DNA fragments used as probes for PFGE gels. ( B ) Hybridization of these probes to gels of DNA from control cells ( C ) or cells irradiated with 100 Gy (Irrad) restricted by the same enzyme; the PFGE conditions differed according to the length of fragments to be detected. Arrows show fragments predicted from the minichromosome DNA sequence; the origin of the weakly-hybridizing fragments is discussed in the text. (C) Fibre-FISH; the hybridization probes and procedure were as described in Figure 2 E. Images show representative linear molecules from irradiated cells (100 Gy); probes are green and DNA is red, and the extremities of the molecule represent the site of breakage. ( D ) Distribution of the breakage site expressed as the % of 55 circular DNA molecules in which the break occurred in one of four quadrants.

    Journal: Nucleic Acids Research

    Article Title: DNA of a circular minichromosome linearized by restriction enzymes or other reagents is resistant to further cleavage: an influence of chromatin topology on the accessibility of DNA

    doi: 10.1093/nar/gks723

    Figure Lengend Snippet: Sites of breakage of minichromosome DNA in γ-irradiated cells. ( A ) SpeI and SwaI EBV DNA fragments used as probes for PFGE gels. ( B ) Hybridization of these probes to gels of DNA from control cells ( C ) or cells irradiated with 100 Gy (Irrad) restricted by the same enzyme; the PFGE conditions differed according to the length of fragments to be detected. Arrows show fragments predicted from the minichromosome DNA sequence; the origin of the weakly-hybridizing fragments is discussed in the text. (C) Fibre-FISH; the hybridization probes and procedure were as described in Figure 2 E. Images show representative linear molecules from irradiated cells (100 Gy); probes are green and DNA is red, and the extremities of the molecule represent the site of breakage. ( D ) Distribution of the breakage site expressed as the % of 55 circular DNA molecules in which the break occurred in one of four quadrants.

    Article Snippet: Restriction fragments of this DNA cut with SpeI or SwaI (100 U/ml) were separated by PFGE in 1% LMP agarose at 190 V/cm for 7 or 20 h and switch time ramped linearly from 0.4 to 6 or 0.3 to 3 s, respectively, excised from gels and purified on Ultrafree columns (Millipore).

    Techniques: Irradiation, Hybridization, Sequencing, Fluorescence In Situ Hybridization

    PCR-RFLP patterns of COI region from JBS samples (D1–D11) digested with Spe I and Bst EII, DNA marker (M) in bp were indicated.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: DNA Barcode-Based PCR-RFLP and Diagnostic PCR for Authentication of Jinqian Baihua She (Bungarus Parvus)

    doi: 10.1155/2015/402820

    Figure Lengend Snippet: PCR-RFLP patterns of COI region from JBS samples (D1–D11) digested with Spe I and Bst EII, DNA marker (M) in bp were indicated.

    Article Snippet: Digestions with Spe I and Bst EII (Takara) were performed in a total volume of 20 μ L containing 2 μ L PCR products, 0.8 μ L enzymes, 14.4 μ L ddH2 O, and 2 μ L 10 × digestion buffer at 37°C for 1 h as recommended by the manufacturer.

    Techniques: Polymerase Chain Reaction, Marker

    PCR-RFLP patterns of COI region digested with Spe I and Bst EII. BM1 (1), BM2 (2), BM3 (3), BM4 (4), BF (5), NJ (6), DR1 (7), DR2 (8), OM (9), EP (10), SA (11), DA (12), DF (13), ZY (14), XF (15), negative control (16) (water was used as sample), and DNA marker (M) in bp were indicated.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: DNA Barcode-Based PCR-RFLP and Diagnostic PCR for Authentication of Jinqian Baihua She (Bungarus Parvus)

    doi: 10.1155/2015/402820

    Figure Lengend Snippet: PCR-RFLP patterns of COI region digested with Spe I and Bst EII. BM1 (1), BM2 (2), BM3 (3), BM4 (4), BF (5), NJ (6), DR1 (7), DR2 (8), OM (9), EP (10), SA (11), DA (12), DF (13), ZY (14), XF (15), negative control (16) (water was used as sample), and DNA marker (M) in bp were indicated.

    Article Snippet: Digestions with Spe I and Bst EII (Takara) were performed in a total volume of 20 μ L containing 2 μ L PCR products, 0.8 μ L enzymes, 14.4 μ L ddH2 O, and 2 μ L 10 × digestion buffer at 37°C for 1 h as recommended by the manufacturer.

    Techniques: Polymerase Chain Reaction, Negative Control, Marker

    Blind evaluation of MSP-1 PCR-RFLP method for Korean P. vivax isolates. The MSP-1 polymorphic region (block 5–6) was amplified from genomic DNA purified from 50 P. vivax Korean isolates by PCR, respectively. Each PCR product was digested with Pvu II , Spe I, and Sty I and analyzed on a 3% agarose gel. The nucleotide sequence of each amplicon was also confirmed to evaluate the accuracy of the method. Only 32 representative results were presented.

    Journal: The Korean Journal of Parasitology

    Article Title: PCR-RFLP for Rapid Subtyping of Plasmodium vivax Korean Isolates

    doi: 10.3347/kjp.2017.55.2.159

    Figure Lengend Snippet: Blind evaluation of MSP-1 PCR-RFLP method for Korean P. vivax isolates. The MSP-1 polymorphic region (block 5–6) was amplified from genomic DNA purified from 50 P. vivax Korean isolates by PCR, respectively. Each PCR product was digested with Pvu II , Spe I, and Sty I and analyzed on a 3% agarose gel. The nucleotide sequence of each amplicon was also confirmed to evaluate the accuracy of the method. Only 32 representative results were presented.

    Article Snippet: PCR products of each subtype MSP-1 were digested with Pvu II, Spe I, and Sty I (each 3 U; Takara) in a total volume of 30 μL at 37°C for 2 hr.

    Techniques: Polymerase Chain Reaction, Blocking Assay, Amplification, Purification, Agarose Gel Electrophoresis, Sequencing

    PCR-RFLP subtyping method for MSP-1 of Korean P. vivax isolates. (A) Schematic view for predicted restriction positions and expected sizes of restriction fragments of the 7 subtypes of Korean P. vivax MSP-1 by 3 restriction enzymes, Pvu II , Spe I, and Sty I. Subtypes A–B, Sal I types; subtypes C–E, recombinant types; subtypes F–G, Belem types. Predicted restriction positions for MSP-1 sequences of Sal I (AF435593) and Belem (AF435594) were also presented. (B) Amplification and PCR-RFLP analysis of the 7 subtypes of Korean P. vivax MSP-1. (Upper panel) PCR amplifications of each subtype MSP-1. Polymorphic region (block 5–6) of MSP-1 was amplified from each subtype and analyzed. (Lower panel) RFLP patterns for each MSP-1 subtype amplicon generated by digestion with Pvu II , Spe I, and Sty I. Different patterns of digested fragments for each subtype were identified. Lane M, 100 bp DNA ladder; lanes A–G, subtypes A to G, respectively.

    Journal: The Korean Journal of Parasitology

    Article Title: PCR-RFLP for Rapid Subtyping of Plasmodium vivax Korean Isolates

    doi: 10.3347/kjp.2017.55.2.159

    Figure Lengend Snippet: PCR-RFLP subtyping method for MSP-1 of Korean P. vivax isolates. (A) Schematic view for predicted restriction positions and expected sizes of restriction fragments of the 7 subtypes of Korean P. vivax MSP-1 by 3 restriction enzymes, Pvu II , Spe I, and Sty I. Subtypes A–B, Sal I types; subtypes C–E, recombinant types; subtypes F–G, Belem types. Predicted restriction positions for MSP-1 sequences of Sal I (AF435593) and Belem (AF435594) were also presented. (B) Amplification and PCR-RFLP analysis of the 7 subtypes of Korean P. vivax MSP-1. (Upper panel) PCR amplifications of each subtype MSP-1. Polymorphic region (block 5–6) of MSP-1 was amplified from each subtype and analyzed. (Lower panel) RFLP patterns for each MSP-1 subtype amplicon generated by digestion with Pvu II , Spe I, and Sty I. Different patterns of digested fragments for each subtype were identified. Lane M, 100 bp DNA ladder; lanes A–G, subtypes A to G, respectively.

    Article Snippet: PCR products of each subtype MSP-1 were digested with Pvu II, Spe I, and Sty I (each 3 U; Takara) in a total volume of 30 μL at 37°C for 2 hr.

    Techniques: Polymerase Chain Reaction, Recombinant, Amplification, Blocking Assay, Generated

    (A) PFGE of SpeI-, XbaI-, and HpaI-digested genomic DNA from multidrug-resistant P. aeruginosa IMCJ2.S1. (B) Southern blotting of the same gels with an aac(6′)-Iae probe. Lanes M, HindIII-digested λ phage DNA as a size marker.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Multidrug-Resistant Pseudomonas aeruginosa Strain That Caused an Outbreak in a Neurosurgery Ward and Its aac(6′)-Iae Gene Cassette Encoding a Novel Aminoglycoside Acetyltransferase

    doi: 10.1128/AAC.49.9.3734-3742.2005

    Figure Lengend Snippet: (A) PFGE of SpeI-, XbaI-, and HpaI-digested genomic DNA from multidrug-resistant P. aeruginosa IMCJ2.S1. (B) Southern blotting of the same gels with an aac(6′)-Iae probe. Lanes M, HindIII-digested λ phage DNA as a size marker.

    Article Snippet: Genomic DNA from P. aeruginosa was prepared by the procedure of Grundmann et al. ( ) and digested overnight with 10 U of SpeI, XbaI, or HpaI (Takara Bio).

    Techniques: Southern Blot, Marker

    Sequence confirmation of the PCR products. A: Nucleotide ‘T’ of the wild type was substituted by ‘C’ of the mutant Jiangtangdao 1; B: The substitution resulted in a missense from Leu-599 of the wild in SBE3 coding region for 599 amino acid to Pro-599 of Jiangtangdao 1 in sbe3-rs coding for high resistant starch; and C Because the point mutation resulted in the loss of a restriction enzyme site Spe I, sbe3-rs of the mutant was not digested with Spe I, while SBE3 of the wild type was (M: marker DL2000; Lane 1–2: wild type before and after digestion; and lane 3–4: Jiangtangdao 1 before and after digestion).

    Journal: PLoS ONE

    Article Title: A Putative Gene sbe3-rs for Resistant Starch Mutated from SBE3 for Starch Branching Enzyme in Rice (Oryza sativa L.)

    doi: 10.1371/journal.pone.0043026

    Figure Lengend Snippet: Sequence confirmation of the PCR products. A: Nucleotide ‘T’ of the wild type was substituted by ‘C’ of the mutant Jiangtangdao 1; B: The substitution resulted in a missense from Leu-599 of the wild in SBE3 coding region for 599 amino acid to Pro-599 of Jiangtangdao 1 in sbe3-rs coding for high resistant starch; and C Because the point mutation resulted in the loss of a restriction enzyme site Spe I, sbe3-rs of the mutant was not digested with Spe I, while SBE3 of the wild type was (M: marker DL2000; Lane 1–2: wild type before and after digestion; and lane 3–4: Jiangtangdao 1 before and after digestion).

    Article Snippet: The sequence result was also verified by restriction enzyme Spe I digestion (TakaRa, Dalian, China).

    Techniques: Sequencing, Polymerase Chain Reaction, Mutagenesis, Marker

    Histamine release and degranulation from HMC-1 cells stimulated with rSPE B/SCP. HMC-1 cells were incubated at 2 × 10 6 cells/ml in Tyrode's solution for 20 min at 37°C with rSPE B/SCP, SPE A, and LPS. After incubation, histamine in the supernatant was measured by enzyme-linked immunosorbent assay. (A) Data are expressed as a percentage of release of histamine in comparison to control. As a control, the HMC-1 cells were allowed to freeze and thaw for three cycles to release the remaining histamine from the cells. (B) Degranulation pattern from HMC-1 cells stimulated with rSPE B/SCP. Panel 1, control (nonstimulated HMC-1 cells); panel 2, rSPE B/SCP-stimulated HMC-1 cells.

    Journal: Infection and Immunity

    Article Title: Cysteine Protease Activity and Histamine Release from the Human Mast Cell Line HMC-1 Stimulated by Recombinant Streptococcal Pyrogenic Exotoxin B/Streptococcal Cysteine Protease

    doi: 10.1128/IAI.70.7.3944-3947.2002

    Figure Lengend Snippet: Histamine release and degranulation from HMC-1 cells stimulated with rSPE B/SCP. HMC-1 cells were incubated at 2 × 10 6 cells/ml in Tyrode's solution for 20 min at 37°C with rSPE B/SCP, SPE A, and LPS. After incubation, histamine in the supernatant was measured by enzyme-linked immunosorbent assay. (A) Data are expressed as a percentage of release of histamine in comparison to control. As a control, the HMC-1 cells were allowed to freeze and thaw for three cycles to release the remaining histamine from the cells. (B) Degranulation pattern from HMC-1 cells stimulated with rSPE B/SCP. Panel 1, control (nonstimulated HMC-1 cells); panel 2, rSPE B/SCP-stimulated HMC-1 cells.

    Article Snippet: After washing with Tyrode's solution, 2 × 106 cells/ml were stimulated with rSPE B/SCP, SPE A (Toxin Technology, Inc., Sarasota, Fla.), or lipopolysaccharide (LPS) (Sigma Chemical Co.).

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay

    Characterization of Pak1 −/− bone marrow–derived mast cells . (A) Bone marrow–derived mast cell (BMMC) receptor expression. BMMCs were maintained in culture for 5 weeks, and expression of c-kit and FcϵRI was measured by incubation with antimouse CD 117 (c-kit) PE-conjugated antibody, and anti-DNP monoclonal antibody IgE clone SPE-7 followed by incubation with FITC-conjugated anti–mouse IgE secondary antibody. Double-positive cells (top right quadrant) are mature mast cells, expressing both c-kit and FcϵRI. Data shown are representative of 6 independent lines from each genotype. (Mean WT = 96.1 + 2.3 SEM % vs Pak1 −/− = 95.3 + 1.7 SEM % double-positive cells, n = 6.) (B) IgE-mediated Pak1 activation in BMMCs (representative of 3 independent experiments). IgE-primed BMMCs were stimulated with antigen (DNP) for the indicated times, lysates were precipitated with anti-Pak1 antibody, and Pak1 activity was assayed. (C) Pak1 activation of pS298-MEK1 in Wt and Pak1 −/− BMMCs. IgE-primed BMMCs were stimulated with antigen (DNP) for the indicated times. Cell lysates (“Western blotting”) were subjected to immunoblotting with anti–phospho-S298 MEK1 (top blot) or anti–total MEK1/2 (bottom blot). (D) Effect of IPA-3 treatment in Wt BMMCs. The length of activation and the addition of inhibitor are indicated.

    Journal: Blood

    Article Title: p21-activated kinase regulates mast cell degranulation via effects on calcium mobilization and cytoskeletal dynamics

    doi: 10.1182/blood-2008-06-160861

    Figure Lengend Snippet: Characterization of Pak1 −/− bone marrow–derived mast cells . (A) Bone marrow–derived mast cell (BMMC) receptor expression. BMMCs were maintained in culture for 5 weeks, and expression of c-kit and FcϵRI was measured by incubation with antimouse CD 117 (c-kit) PE-conjugated antibody, and anti-DNP monoclonal antibody IgE clone SPE-7 followed by incubation with FITC-conjugated anti–mouse IgE secondary antibody. Double-positive cells (top right quadrant) are mature mast cells, expressing both c-kit and FcϵRI. Data shown are representative of 6 independent lines from each genotype. (Mean WT = 96.1 + 2.3 SEM % vs Pak1 −/− = 95.3 + 1.7 SEM % double-positive cells, n = 6.) (B) IgE-mediated Pak1 activation in BMMCs (representative of 3 independent experiments). IgE-primed BMMCs were stimulated with antigen (DNP) for the indicated times, lysates were precipitated with anti-Pak1 antibody, and Pak1 activity was assayed. (C) Pak1 activation of pS298-MEK1 in Wt and Pak1 −/− BMMCs. IgE-primed BMMCs were stimulated with antigen (DNP) for the indicated times. Cell lysates (“Western blotting”) were subjected to immunoblotting with anti–phospho-S298 MEK1 (top blot) or anti–total MEK1/2 (bottom blot). (D) Effect of IPA-3 treatment in Wt BMMCs. The length of activation and the addition of inhibitor are indicated.

    Article Snippet: Cells were blocked with unconjugated anti-FcγRII/III (BD Pharmingen, San Diego, CA) and stained with anti-DNP monoclonal antibody IgE clone SPE-7 (Sigma-Aldrich), anti–mouse CD 117 (c-kit) PE-conjugated antibody, and FITC-conjugated anti–mouse IgE (both BD Pharmingen) secondary antibody.

    Techniques: Derivative Assay, Expressing, Incubation, Activation Assay, Activity Assay, Indirect Immunoperoxidase Assay

    Outline of the high resolution nano-LC-MS/MS method for the analysis of NNAL, NNAL O-glucuronide and NNAL N-glucuronide in smoker’s urine. a SPE = solid phase extraction.

    Journal: Chemical research in toxicology

    Article Title: Influence of UGT2B10 genotype on urinary excretion of NNAL-N-glucuronide by African American smokers

    doi: 10.1021/acs.chemrestox.7b00264

    Figure Lengend Snippet: Outline of the high resolution nano-LC-MS/MS method for the analysis of NNAL, NNAL O-glucuronide and NNAL N-glucuronide in smoker’s urine. a SPE = solid phase extraction.

    Article Snippet: However, due to the highly polar nature of the NNAL N- glucuronide, and despite significant effort, we were unable with any SPE column to separate this NNAL metabolite from the many equally polar compounds in urine which result in significant ion suppression during LC-MS/MS analysis.

    Techniques: Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Comparison of methods used to extract marine toxins tetrodotoxin (TTX), saxitoxin (STX), and neosaxitoxin (NEO): A) All analytes extracted from urine matrix using Oasis MCX online SPE cartridge and Halo Penta-HILIC LC column. B) All analytes extracted from urine matrix using Oasis WCX online SPE cartridge and Atlantis Silica HILIC LC column.

    Journal: Toxicon : official journal of the International Society on Toxinology

    Article Title: Development and validation of a high-throughput online solid phase extraction – Liquid chromatography – Tandem mass spectrometry method for the detection of tetrodotoxin in human urine

    doi: 10.1016/j.toxicon.2016.05.009

    Figure Lengend Snippet: Comparison of methods used to extract marine toxins tetrodotoxin (TTX), saxitoxin (STX), and neosaxitoxin (NEO): A) All analytes extracted from urine matrix using Oasis MCX online SPE cartridge and Halo Penta-HILIC LC column. B) All analytes extracted from urine matrix using Oasis WCX online SPE cartridge and Atlantis Silica HILIC LC column.

    Article Snippet: Oasis MCX 10 × 1 mm, 30 μm particle size, SPE cartridges were purchased from Waters Corporation (Milford, MA).

    Techniques: Hydrophilic Interaction Liquid Chromatography

    Evaluation of 2DLC configuration. NAc-linker-DSEA, spiked into a dilute trastuzumab sample, was successfully transferred from ( A ) the SPE column (1 st dimension) to the ( B ) RP column (2 nd dimension) using the 2DLC configuration illustrated in Figure 4 A as proof-of-principle.

    Journal: mAbs

    Article Title: A sensitive multidimensional method for the detection, characterization, and quantification of trace free drug species in antibody-drug conjugate samples using mass spectral detection

    doi: 10.1080/19420862.2015.1116659

    Figure Lengend Snippet: Evaluation of 2DLC configuration. NAc-linker-DSEA, spiked into a dilute trastuzumab sample, was successfully transferred from ( A ) the SPE column (1 st dimension) to the ( B ) RP column (2 nd dimension) using the 2DLC configuration illustrated in Figure 4 A as proof-of-principle.

    Article Snippet: The spiked trastuzumab sample was injected onto the SPE column at a flow rate of 0.100 ml/min with an initial MP composition of 23% MP B (1st dimension MP reservoirs).

    Techniques:

    Method evaluation of SPE with spiked sample. Mal-linker-DSEA, and NAc-linker-DSEA was spiked into a dilute trastuzumab sample for SPE optimization. Optimal SPE loading conditions for the extraction of mal-linker-DSEA and NAc-linker-DSEA components from the spiked AFC sample were determined to be 18% acetonitrile containing 2% FA v/v. A step gradient to 36% acetonitrile containing 2% FA v/v was determined to be optimal conditions to elute bound drug components in a narrow peak centered around 13.5 min.

    Journal: mAbs

    Article Title: A sensitive multidimensional method for the detection, characterization, and quantification of trace free drug species in antibody-drug conjugate samples using mass spectral detection

    doi: 10.1080/19420862.2015.1116659

    Figure Lengend Snippet: Method evaluation of SPE with spiked sample. Mal-linker-DSEA, and NAc-linker-DSEA was spiked into a dilute trastuzumab sample for SPE optimization. Optimal SPE loading conditions for the extraction of mal-linker-DSEA and NAc-linker-DSEA components from the spiked AFC sample were determined to be 18% acetonitrile containing 2% FA v/v. A step gradient to 36% acetonitrile containing 2% FA v/v was determined to be optimal conditions to elute bound drug components in a narrow peak centered around 13.5 min.

    Article Snippet: The spiked trastuzumab sample was injected onto the SPE column at a flow rate of 0.100 ml/min with an initial MP composition of 23% MP B (1st dimension MP reservoirs).

    Techniques:

    PFGE fingerprints of 45 E. aerogenes isolates (1995) after digestion with the Xba I restriction enzyme (A) and the Spe I restriction enzyme (B). Lane FH54 to lane BG64, profile IA and profile IB (broadly clone I); lane FH51 to lane FH90, profiles IIA, IIB, and IIC (broadly, clone II); lane FH49 to lane FH77, unrelated strains (nonclonal); lanes M, molecular size markers (PFGE marker I, λ ladder).

    Journal: Journal of Clinical Microbiology

    Article Title: Molecular Epidemiological Study of Nosocomial Enterobacter aerogenes Isolates in a Belgian Hospital

    doi:

    Figure Lengend Snippet: PFGE fingerprints of 45 E. aerogenes isolates (1995) after digestion with the Xba I restriction enzyme (A) and the Spe I restriction enzyme (B). Lane FH54 to lane BG64, profile IA and profile IB (broadly clone I); lane FH51 to lane FH90, profiles IIA, IIB, and IIC (broadly, clone II); lane FH49 to lane FH77, unrelated strains (nonclonal); lanes M, molecular size markers (PFGE marker I, λ ladder).

    Article Snippet: Moreover, use of the Xba I restriction enzyme was more cost-effective than use of Spe I (about 1/10 the price of Spe I [GIBCO-BRL]).

    Techniques: IA, Marker

    PFGE patterns of genomic DNAs of B. lactis strains after Spe I digestion. Lanes: 1, B. lactis NCC 383; 2, B. lactis NCC 363; 3, B. lactis DSM 10140; 4, B. lactis NCC 402; 5, B. lactis DSM 10140; 6, B. lactis NCC 239; 7, B. lactis NCC 387; 8, B. lactis NCC 311; 9, B. lactis DSM 10140; M, λ DNA ladder (Bio-Rad).

    Journal: Applied and Environmental Microbiology

    Article Title: Rapid Identification, Differentiation, and Proposed New Taxonomic Classification of Bifidobacterium lactis

    doi: 10.1128/AEM.68.12.6429-6434.2002

    Figure Lengend Snippet: PFGE patterns of genomic DNAs of B. lactis strains after Spe I digestion. Lanes: 1, B. lactis NCC 383; 2, B. lactis NCC 363; 3, B. lactis DSM 10140; 4, B. lactis NCC 402; 5, B. lactis DSM 10140; 6, B. lactis NCC 239; 7, B. lactis NCC 387; 8, B. lactis NCC 311; 9, B. lactis DSM 10140; M, λ DNA ladder (Bio-Rad).

    Article Snippet: For digestion with restriction endonucleases, cells in agarose blocks were treated with 50 U (each) of Xba I and Spe I (Roche Molecular).

    Techniques:

    Electropherograms obtained during the electrophoretic separation of human urine samples containing eight steroid hormones after SPE using C 18 cartridges and elution of steroids with: methanol ( A ), and dichloromethane ( B ), next SPE using HLB cartridges and elution of steroids with methanol ( C ), dichloromethane ( D ). Conditions: UV detection at 254 nm, unmodified silica capillary (57 cm × 50 μm I.D.), temp. 22 °C, running buffer composed of (10:90, v/v) methanol and mixture of 10 mM Na 2 B 4 O 7 and 50 mM SDS.

    Journal: Molecules

    Article Title: Optimization of a Pre-MEKC Separation SPE Procedure for Steroid Molecules in Human Urine Samples

    doi: 10.3390/molecules181114013

    Figure Lengend Snippet: Electropherograms obtained during the electrophoretic separation of human urine samples containing eight steroid hormones after SPE using C 18 cartridges and elution of steroids with: methanol ( A ), and dichloromethane ( B ), next SPE using HLB cartridges and elution of steroids with methanol ( C ), dichloromethane ( D ). Conditions: UV detection at 254 nm, unmodified silica capillary (57 cm × 50 μm I.D.), temp. 22 °C, running buffer composed of (10:90, v/v) methanol and mixture of 10 mM Na 2 B 4 O 7 and 50 mM SDS.

    Article Snippet: After the urine samples were centrifuged, the solution was rapidly passed through the SPE cartridges (Merck, LiChrolut RP-18, 500 mg or HLB), preconditioned with methanol (5 mL) and washed twice with deionized water (5 mL), using a vacuum.

    Techniques: