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Image Search Results
Journal: Advanced Materials Interfaces
Article Title: Bioinspired Hyaluronic Acid‐Based Hydrogel Fuels Bi‐Directional Lung Organoid Maturation via PIEZO1 and ITGB1 Mediated Mechanosensation
doi: 10.1002/admi.202400194
Figure Lengend Snippet: Figure 5. Mechanosensation of lung organoids growing in HA-PEGDT-Matrigel. A) Representative images showing the expression of PIZEO1 (green), RRAS (green), Act-ITGB1 (white) and NMHC IIA (red) in AFE encapsulated in the HA-PEGDT-Matrigel for 8 days (day 16 in total post induction) com- pared to those in the Matrigel group. The nuclei (cyan) were stained with Hoechst 33 342. Scale bar: 100 μm. B) Quantification of the mean fluorescence intensity alteration in PIZEO1, RRAS, Active ITGB1 and NMHC IIA levels in spheroid from indicated groups after 8-day encapsulation (day 16 in total post induction) (n = 8 for PIEZO1, ActßITGB1 and RRAS; n = 12 for NMHC IIA; ***p < 0.001, ****p < 0.0001, two-tailed unpaired Student t-test). C) Scheme of the proposed underlying mechanism of HA-PEGDT-Matrigel derived lung organoid from spheroid-hydrogel interface to the intracellular events in response to biomechanical stimulation.
Article Snippet: Primary antibody Host/Isotype Supplier Catalog no. Dilution CPM Mouse/IgG1 Thermo Fisher Scientific MA5-26863 1:200 CCSP Rabbit/IgG Sigma-Aldrich SAB2108213 1:100 FOXA2 Rabbit/IgG CST 8186S 1:400 Active ITGB1 Mouse/IgG2b Millipore MAB2079Z 1:200
Techniques: Expressing, Staining, Encapsulation, Two Tailed Test, Derivative Assay
Journal: Advanced Materials Interfaces
Article Title: Bioinspired Hyaluronic Acid‐Based Hydrogel Fuels Bi‐Directional Lung Organoid Maturation via PIEZO1 and ITGB1 Mediated Mechanosensation
doi: 10.1002/admi.202400194
Figure Lengend Snippet: Figure 5. Mechanosensation of lung organoids growing in HA-PEGDT-Matrigel. A) Representative images showing the expression of PIZEO1 (green), RRAS (green), Act-ITGB1 (white) and NMHC IIA (red) in AFE encapsulated in the HA-PEGDT-Matrigel for 8 days (day 16 in total post induction) com- pared to those in the Matrigel group. The nuclei (cyan) were stained with Hoechst 33 342. Scale bar: 100 μm. B) Quantification of the mean fluorescence intensity alteration in PIZEO1, RRAS, Active ITGB1 and NMHC IIA levels in spheroid from indicated groups after 8-day encapsulation (day 16 in total post induction) (n = 8 for PIEZO1, ActßITGB1 and RRAS; n = 12 for NMHC IIA; ***p < 0.001, ****p < 0.0001, two-tailed unpaired Student t-test). C) Scheme of the proposed underlying mechanism of HA-PEGDT-Matrigel derived lung organoid from spheroid-hydrogel interface to the intracellular events in response to biomechanical stimulation.
Article Snippet: Primary antibody Host/Isotype Supplier Catalog no. Dilution CPM Mouse/IgG1 Thermo Fisher Scientific MA5-26863 1:200 CCSP Rabbit/IgG Sigma-Aldrich SAB2108213 1:100 FOXA2 Rabbit/IgG CST 8186S 1:400 Active ITGB1 Mouse/IgG2b Millipore MAB2079Z 1:200 NMHC IIA Rabbit/IgG CST 3403S 1:50 NKX2.1 Rabbit/IgG Thermo Fisher Scientific PA5-25940 1:200 OCT4 Mouse/IgG1 Thermo Fisher Scientific MA1-104 1:500 PIEZO1 Rabbit/IgG Novus Biologicals NBP1-78446 1:100
Techniques: Expressing, Staining, Encapsulation, Two Tailed Test, Derivative Assay