Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Genome-wide analysis of genetic alterations in Barrett's adenocarcinoma using single nucleotide polymorphism arrays.

doi: 10.1038/labinvest.2008.67

Figure Lengend Snippet: Figure 2 Schematic depiction of amplification on chromosome 8. On the upper part, (scaled up view of 8p23.1 (SOX7, GATA4)) green bars display the detected amplicons with their possible maximal extensions to the next non-amplified SNP (white bars). The lower part of the figure shows a zoom into 8q24.21 (MYC) with the same design. On the right of each bar the maximal detected copy number of the respective case is written in parentheses.

Article Snippet: Staining was performed using an anti-human SOX7 goat antibody (R&D Systems, MN, USA, diluted 1:50) and the streptavidin/AP system with biotinylated rabbit anti-goat antiserum (1:200) in an autostainer plus (Dako, Glostrup, Denmark) according to the Dako standard protocol.

Techniques: Amplification

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Genome-wide analysis of genetic alterations in Barrett's adenocarcinoma using single nucleotide polymorphism arrays.

doi: 10.1038/labinvest.2008.67

Figure Lengend Snippet: Figure 3 Merged FISH images (a, c) with probes for centromere 8 (blue), MYC (red), SOX7 (green) and DAPI nuclear counterstain (grey). On the right (b, d) immunohistochemical staining of SOX7 protein of the same tumors is displayed. Case 7 (a, b) had an amplification of the SOX7 gene, a low-level copy- number gain of MYC (a), and a strong nuclear expression of SOX7 (b). In contrast, case 14 (c, d) had no SOX7 amplification, a low-level gain of MYC copies (c), and no immunohistochemical SOX7 staining (d). Overlying columnar (b) or squamous (d) epithelium, as well as lymphocytes and fibroblasts did not show nuclear staining.

Article Snippet: Staining was performed using an anti-human SOX7 goat antibody (R&D Systems, MN, USA, diluted 1:50) and the streptavidin/AP system with biotinylated rabbit anti-goat antiserum (1:200) in an autostainer plus (Dako, Glostrup, Denmark) according to the Dako standard protocol.

Techniques: Immunohistochemical staining, Staining, Amplification, Expressing

Journal: BMC Biology

Article Title: VEGF-dependent testicular vascularisation involves MEK1/2 signalling and the essential angiogenesis factors, SOX7 and SOX17

doi: 10.1186/s12915-024-02003-y

Figure Lengend Snippet: Antibodies for flow cytometric and immunofluorescence (IF) experiments

Article Snippet: SOX7 , R&D Systems, AF2766 , Goat , SIF: 1/800 WIF: 1/800.

Techniques: Immunofluorescence

Journal: International journal of molecular medicine

Article Title: Decreased expression of Sox7 correlates with the upregulation of the Wnt/β-catenin signaling pathway and the poor survival of gastric cancer patients.

doi: 10.3892/ijmm.2014.1759

Figure Lengend Snippet: Figure 1. Sox7 and β-catenin expression in vitro. (A) RT-PCR analysis revealed that Sox7 was expressed at lower levels in the AGS and MKN-45 cells gastric cancer compared the GES-1 normal gastric mucosa cells. (B) Western blot analysis revealed that Sox7 was expressed at lower levels in the AGS and MKN-45 levels compared the GES-1 cells.

Article Snippet: The membranes were incubated at room temperature overnight with the appropriate antibodies [anti-human Sox7 (R&D Systems), anti-human β-catenin (R&D Systems) and anti-human β-actin (Sigma St. Louis, MO, USA)] diluted in a basal solution containing 3% skim milk.

Techniques: Expressing, In Vitro, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: International journal of molecular medicine

Article Title: Decreased expression of Sox7 correlates with the upregulation of the Wnt/β-catenin signaling pathway and the poor survival of gastric cancer patients.

doi: 10.3892/ijmm.2014.1759

Figure Lengend Snippet: Figure 2. Immunohistochemistry (IHC). Sox7 expression was frequently downregulated and correlated with β-catenin expression in the gastric cancer (GC) samples. Sox7 was expressed mainly in the nucleus, and to a lesser extent in the cytoplasm. β-catenin was present in the nucleus, cytoplasm and cell membrane. (A) Sox7 was expressed mainly in the nucleus, and in the cytoplasm to a lesser extent, of distal normal gastric mucosal tissue samples (magnification, x100); (B) β-catenin expression was absent in distal normal gastric mucosal tissue samples (x100); (C and D) Sox7 was weakly expressed at sites where β-catenin expres sion was significantly upregulated in GC (x100); (E and F) Sox7 expression was upregulated at sites where β-catenin expression was absent in GC samples (x100).

Article Snippet: The membranes were incubated at room temperature overnight with the appropriate antibodies [anti-human Sox7 (R&D Systems), anti-human β-catenin (R&D Systems) and anti-human β-actin (Sigma St. Louis, MO, USA)] diluted in a basal solution containing 3% skim milk.

Techniques: Immunohistochemistry, Expressing, Membrane

Journal: International journal of molecular medicine

Article Title: Decreased expression of Sox7 correlates with the upregulation of the Wnt/β-catenin signaling pathway and the poor survival of gastric cancer patients.

doi: 10.3892/ijmm.2014.1759

Figure Lengend Snippet: Figure 3. Western blot analysis of Sox7 expression in fresh-frozen tissues and its correlation with β-catenin expression levels. (A) Western blot analysis revealed that Sox7 was upregulated in the normal tissue samples, and that Sox7 suppressed the expression of β-catenin in normal tissue samples. (B) Sox7 and β-catenin expression levels were significantly different between the two groups (*,**P<0.05). (C) Correlation between Sox7 and β-catenin relative expression levels in gastric cancer. Protein levels were relative to β-actin. There was a significant negative linear correlation between Sox7 and β-catenin protein expression (P<0.001, R=-0.698). N, normal samples; T, tumor samples.

Article Snippet: The membranes were incubated at room temperature overnight with the appropriate antibodies [anti-human Sox7 (R&D Systems), anti-human β-catenin (R&D Systems) and anti-human β-actin (Sigma St. Louis, MO, USA)] diluted in a basal solution containing 3% skim milk.

Techniques: Western Blot, Expressing

Journal: International journal of molecular medicine

Article Title: Decreased expression of Sox7 correlates with the upregulation of the Wnt/β-catenin signaling pathway and the poor survival of gastric cancer patients.

doi: 10.3892/ijmm.2014.1759

Figure Lengend Snippet: Figure 4. Kaplan-Meier survival analysis of Sox7 status (n=256). The blue line indicates Sox7-negative patients. The green line indicates Sox7-positive patients with GC (log-rank =35.99, P=0.001).

Article Snippet: The membranes were incubated at room temperature overnight with the appropriate antibodies [anti-human Sox7 (R&D Systems), anti-human β-catenin (R&D Systems) and anti-human β-actin (Sigma St. Louis, MO, USA)] diluted in a basal solution containing 3% skim milk.

Techniques: