Journal: Gene

Article Title: miRNA-1229-5p promotes migration and invasion and suppresses apoptosis of endometrial cells via the STMN1/p38 MAPK axis in endometriosis.

doi: 10.1016/j.gene.2025.149385

Figure Lengend Snippet: Fig. 4. Mir-1229-5p activated p38 mapk signaling pathway. A-B) Western blotting detected the p38 MAPK signaling related protein expression of p38, p-p38, ATF2, p-ATF2, mmp2, mmp9 in transfected ESCs and ISK cells. *P < 0.05, **P < 0.01.

Article Snippet: This was followed by overnight incubation at 4 ◦C with primary antibodies against E-cadherin, N-cadherin, ZEB1, Bcl-2, Bax, mmp2, mmp9, and GAPDH (all sourced from Proteintech, Rosemont, IL, USA), STMN1 (Abcam, USA), p-p38/p38, and p-ATF2/ATF2 (Santa Cruz, USA).

Techniques: Western Blot, Expressing, Transfection

Journal: Gene

Article Title: miRNA-1229-5p promotes migration and invasion and suppresses apoptosis of endometrial cells via the STMN1/p38 MAPK axis in endometriosis.

doi: 10.1016/j.gene.2025.149385

Figure Lengend Snippet: Fig. 7. The re-expression of STMN1 could reversed the p38 MAPK signaling activation of miR-1229-5p overexpression in ESCs and ISK cells. A-B) Western blotting detected the p38 MAPK signaling related protein expression of p38, p-p38, ATF2, p-ATF2, mmp2, mmp9 in transfected ESCs and ISK cells. *P < 0.05, **P < 0.01.

Article Snippet: This was followed by overnight incubation at 4 ◦C with primary antibodies against E-cadherin, N-cadherin, ZEB1, Bcl-2, Bax, mmp2, mmp9, and GAPDH (all sourced from Proteintech, Rosemont, IL, USA), STMN1 (Abcam, USA), p-p38/p38, and p-ATF2/ATF2 (Santa Cruz, USA).

Techniques: Expressing, Activation Assay, Over Expression, Western Blot, Transfection

Journal: Blood Cancer Journal

Article Title: Synergistic cytotoxicity of gemcitabine, clofarabine and edelfosine in lymphoma cell lines

doi: 10.1038/bcj.2013.69

Figure Lengend Snippet: Inhibition of the AKT pro-survival pathway by [Gem+Clo+Ed]. ( a ) J45.01 and ( b ) OCI-LY10 cells were continuously exposed to drugs alone or in combination for 48 h and analyzed by western blotting using the indicated antibodies.

Article Snippet: Most of the antibodies, their sources and other relevant information were previously described., , Antibodies against apoptosis-inducing factor, AKT, p-AKT (Ser473), p-AKT (Thr308), ATF2, p-ATF2 (Thr71), Bax, cleaved caspase 8, c-Jun, p-c-Jun (Ser73), COX 4, cytochrome c , 4E-BP1, p-4E-BP1 (Thr37/46), PDK1, p-PDK1 (Ser241), SAPK/JNK and p-SAPK/JNK (Thr183/Tyr185) were purchased from Cell Signaling Technology.

Techniques: Inhibition, Western Blot

Journal: Blood Cancer Journal

Article Title: Synergistic cytotoxicity of gemcitabine, clofarabine and edelfosine in lymphoma cell lines

doi: 10.1038/bcj.2013.69

Figure Lengend Snippet: Suggested mechanisms of synergistic cytotoxicity of [Gem+Clo+Ed] combination in lymphoma cells based on the previously reported data and our present results. Edelfosine accumulates in lipid rafts in the cell membrane, leads to formation of death-inducing signaling complex (DISC), activates the extrinsic apoptotic pathway through FADD and caspase (Casp) 8, and inhibits the AKT pro-survival pathway. Edelfosine also decreases the mitochondrial membrane potential leading to activation of the intrinsic apoptotic pathway through the release of pro-apoptotic factors from the mitochondria and activation of caspases 9 and 3. [Gem+Clo] combination, on the other hand, inhibits DNA synthesis and repair leading to a DNA-damage response and apoptosis via the intrinsic pathway. The combination of [Gem+Clo+Ed] magnifies the decrease in the mitochondrial membrane potential, production of ROS and activation of apoptosis. Furthermore, drug-mediated activation of the SAPK/JNK signal transduction pathway through upregulation of ATF2 and c-Jun leads to inhibition of survival.

Article Snippet: Most of the antibodies, their sources and other relevant information were previously described., , Antibodies against apoptosis-inducing factor, AKT, p-AKT (Ser473), p-AKT (Thr308), ATF2, p-ATF2 (Thr71), Bax, cleaved caspase 8, c-Jun, p-c-Jun (Ser73), COX 4, cytochrome c , 4E-BP1, p-4E-BP1 (Thr37/46), PDK1, p-PDK1 (Ser241), SAPK/JNK and p-SAPK/JNK (Thr183/Tyr185) were purchased from Cell Signaling Technology.

Techniques: Membrane, Activation Assay, DNA Synthesis, Transduction, Inhibition