soluble il-6 rα Search Results


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  • 93
    R&D Systems soluble il 6rα
    IL-6 signaling is critical for chemotaxis of DCs. A , Higher IL-6 signaling of WT BMDCs than that of fascin1 KO BMDCs. Proximity Ligation Assay (PLA) was used to determine the in situ association between <t>IL-6Rα</t> and gp130, representing the extent of IL-6 signaling. a b, immature BMDCs; c d, mature BMDCs. a c, WT. b d, fascin1 KO. Fluorescence speckles (arrows) indicate the association between IL-6Rα and gp130. Cell boundaries are indicated by dashed lines. B , Quantitative analyses of PLA signals in WT and fascin1 KO BMDCs (n=51). **, p
    Soluble Il 6rα, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soluble il 6rα/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    soluble il 6rα - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    94
    R&D Systems recombinant human soluble il 6r protein
    IL-6 signaling is critical for chemotaxis of DCs. A , Higher IL-6 signaling of WT BMDCs than that of fascin1 KO BMDCs. Proximity Ligation Assay (PLA) was used to determine the in situ association between <t>IL-6Rα</t> and gp130, representing the extent of IL-6 signaling. a b, immature BMDCs; c d, mature BMDCs. a c, WT. b d, fascin1 KO. Fluorescence speckles (arrows) indicate the association between IL-6Rα and gp130. Cell boundaries are indicated by dashed lines. B , Quantitative analyses of PLA signals in WT and fascin1 KO BMDCs (n=51). **, p
    Recombinant Human Soluble Il 6r Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human soluble il 6r protein/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant human soluble il 6r protein - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    85
    R&D Systems biotinylated polyclonal goat anti human soluble il 6 receptor
    IL-6 signaling is critical for chemotaxis of DCs. A , Higher IL-6 signaling of WT BMDCs than that of fascin1 KO BMDCs. Proximity Ligation Assay (PLA) was used to determine the in situ association between <t>IL-6Rα</t> and gp130, representing the extent of IL-6 signaling. a b, immature BMDCs; c d, mature BMDCs. a c, WT. b d, fascin1 KO. Fluorescence speckles (arrows) indicate the association between IL-6Rα and gp130. Cell boundaries are indicated by dashed lines. B , Quantitative analyses of PLA signals in WT and fascin1 KO BMDCs (n=51). **, p
    Biotinylated Polyclonal Goat Anti Human Soluble Il 6 Receptor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated polyclonal goat anti human soluble il 6 receptor/product/R&D Systems
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated polyclonal goat anti human soluble il 6 receptor - by Bioz Stars, 2021-05
    85/100 stars
      Buy from Supplier

    N/A
    Purified recombinant Human IL6R alpha protein
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    N/A
    The protein encoded by this gene belongs to the type I cytokine receptor family This receptor with homology to gp130 is expressed on monocytes and is involved in IL 31
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    N/A
    Sarilumab Kevzara is a fully human anti IL 6R monoclonal IgG1 antibody that can bind to membrane as well as soluble forms of interleukin 6 receptor IL 6R This leads
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    N/A
    IL 6 mediates its biological effects through the type I IL 6 receptor system that consists of two chains IL 6Rα and gp130 The IL 6Rα chain is the binding
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    Image Search Results


    IL-6 signaling is critical for chemotaxis of DCs. A , Higher IL-6 signaling of WT BMDCs than that of fascin1 KO BMDCs. Proximity Ligation Assay (PLA) was used to determine the in situ association between IL-6Rα and gp130, representing the extent of IL-6 signaling. a b, immature BMDCs; c d, mature BMDCs. a c, WT. b d, fascin1 KO. Fluorescence speckles (arrows) indicate the association between IL-6Rα and gp130. Cell boundaries are indicated by dashed lines. B , Quantitative analyses of PLA signals in WT and fascin1 KO BMDCs (n=51). **, p

    Journal: bioRxiv

    Article Title: Investigation of fascin1, a marker of mature dendritic cells, reveals a New role for IL-6 signaling in chemotaxis

    doi: 10.1101/2020.03.19.979104

    Figure Lengend Snippet: IL-6 signaling is critical for chemotaxis of DCs. A , Higher IL-6 signaling of WT BMDCs than that of fascin1 KO BMDCs. Proximity Ligation Assay (PLA) was used to determine the in situ association between IL-6Rα and gp130, representing the extent of IL-6 signaling. a b, immature BMDCs; c d, mature BMDCs. a c, WT. b d, fascin1 KO. Fluorescence speckles (arrows) indicate the association between IL-6Rα and gp130. Cell boundaries are indicated by dashed lines. B , Quantitative analyses of PLA signals in WT and fascin1 KO BMDCs (n=51). **, p

    Article Snippet: To restore IL-6 signaling of IL-6Rα KO DCs, human IL-6 (15ng/ml, 206-IL, R & D Systems) and soluble IL-6Rα (sIL-6Rα, 25ng/ml, 227-SR, R & D Systems) were added 1hr after LPS addition.

    Techniques: Chemotaxis Assay, Proximity Ligation Assay, In Situ, Fluorescence

    IL-6Rα signaling is critical for CCR7 internalization and CCL19-induced ERK1/2 phosphorylation. A , Effects of the blockage of IL-6 signaling on CCR7 internalization. WT BMDCs were stimulated with CCL19 for 5 min in the presence of either control (a) or neutralizing antibody (b), and then surface CCR7 expression was determined by flow cytometry. Surface expression of CCR7 was examined with CD86 + BMDCs before (-CCL19) and after CCL19 addition (+CCL19). Note that CCR7 internalization was blocked in the absence of a neutralizing antibody to IL-6Rα. B , Effects of IL-6 signaling on CCR7 internalization of IL-6Rα KO BMDCs. IL-6Rα KO BMDCs were stimulated with CCL19 in the absence (a) or presence of sIL-6Rα (soluble IL-6Rα) and IL-6 (b). Note that IL-6Rα KO BMDCs showed impaired CCR7 internalization (a), which was rescued by the addition of sIL-6Rα and IL-6 (b). C , Inhibition of CCR7 internalization by the blockage of IL-6 signaling in HEK293T epithelial cells. HEK293T cells stably expressing mouse CCR7-GFP were stimulated with CCL19 in the presence of either control or neutralizing antibody against IL-6Rα. a-d, fluorescence images of CCR7-GFP in control cells (a b) or IL-6Rα neutralizing antibody-treated cells (c d) before (a c) or after addition of CCL19 (b d). The same cells were counter stained with an antibody specific to mouse CCR7 to detect only surface CCR7 (e-h) in control (e f) or IL-6Rα neutralizing antibody-treated cells (g h) before (e g) or after (f h) addition of CCL19. D , Quantitative measurements of surface CCR7 immunofluorescence (panels e-h of C) at cell-to-cell contacts. In contrast to the control, the addition of a neutralizing antibody against IL-6Rα blocked internalization of CCR7. E , Effects of the blockage of IL-6 signaling on CCL19-induced ERK1/2 phosphorylation of WT BMDCs. WT BMDCs were stimulated with CCL19 in the presence of a control or neutralizing antibody, then ERK1/2 phosphorylation levels were determined at 0, 5, 15 and 30min after CCL19 addition using Western blotting with a phosphospecific ERK1/2 antibody. For normalization, the same membranes were reblotted with a pan ERK1/2 antibody. The ratios of phosphoERK1/2 to total ERK1/2 are indicated below the figure.

    Journal: bioRxiv

    Article Title: Investigation of fascin1, a marker of mature dendritic cells, reveals a New role for IL-6 signaling in chemotaxis

    doi: 10.1101/2020.03.19.979104

    Figure Lengend Snippet: IL-6Rα signaling is critical for CCR7 internalization and CCL19-induced ERK1/2 phosphorylation. A , Effects of the blockage of IL-6 signaling on CCR7 internalization. WT BMDCs were stimulated with CCL19 for 5 min in the presence of either control (a) or neutralizing antibody (b), and then surface CCR7 expression was determined by flow cytometry. Surface expression of CCR7 was examined with CD86 + BMDCs before (-CCL19) and after CCL19 addition (+CCL19). Note that CCR7 internalization was blocked in the absence of a neutralizing antibody to IL-6Rα. B , Effects of IL-6 signaling on CCR7 internalization of IL-6Rα KO BMDCs. IL-6Rα KO BMDCs were stimulated with CCL19 in the absence (a) or presence of sIL-6Rα (soluble IL-6Rα) and IL-6 (b). Note that IL-6Rα KO BMDCs showed impaired CCR7 internalization (a), which was rescued by the addition of sIL-6Rα and IL-6 (b). C , Inhibition of CCR7 internalization by the blockage of IL-6 signaling in HEK293T epithelial cells. HEK293T cells stably expressing mouse CCR7-GFP were stimulated with CCL19 in the presence of either control or neutralizing antibody against IL-6Rα. a-d, fluorescence images of CCR7-GFP in control cells (a b) or IL-6Rα neutralizing antibody-treated cells (c d) before (a c) or after addition of CCL19 (b d). The same cells were counter stained with an antibody specific to mouse CCR7 to detect only surface CCR7 (e-h) in control (e f) or IL-6Rα neutralizing antibody-treated cells (g h) before (e g) or after (f h) addition of CCL19. D , Quantitative measurements of surface CCR7 immunofluorescence (panels e-h of C) at cell-to-cell contacts. In contrast to the control, the addition of a neutralizing antibody against IL-6Rα blocked internalization of CCR7. E , Effects of the blockage of IL-6 signaling on CCL19-induced ERK1/2 phosphorylation of WT BMDCs. WT BMDCs were stimulated with CCL19 in the presence of a control or neutralizing antibody, then ERK1/2 phosphorylation levels were determined at 0, 5, 15 and 30min after CCL19 addition using Western blotting with a phosphospecific ERK1/2 antibody. For normalization, the same membranes were reblotted with a pan ERK1/2 antibody. The ratios of phosphoERK1/2 to total ERK1/2 are indicated below the figure.

    Article Snippet: To restore IL-6 signaling of IL-6Rα KO DCs, human IL-6 (15ng/ml, 206-IL, R & D Systems) and soluble IL-6Rα (sIL-6Rα, 25ng/ml, 227-SR, R & D Systems) were added 1hr after LPS addition.

    Techniques: Expressing, Flow Cytometry, Inhibition, Stable Transfection, Fluorescence, Staining, Immunofluorescence, Western Blot

    IL-6 signaling is required for directional migration of BMDCs. A, Analyses of migration tracks. Live cell imaging of BMDCs migrating in a collagen gel toward CCL19 was performed to obtain migration tracks (a, c e) and rose diagram plots for directionality(b, d f). a b, WT (n=19): c d, fascin1 KO (n=23): e f, WT BMDCs in the presence of a neutralizing antibody against IL-6Rα (n=28). WT BMDCs (a b) showed more consistent migration toward CCL19 than fascin1 KO counterparts (c d). Blockage of IL-6 signaling inhibits directed migration of WT BMDCs (e f). Arrows, the direction of a CCL19 gradient. B-E, Box plot analyses of parallel (B, FMI∥) and perpendicular (C, FMI⊥) forward migration indexes, directness (D), and migration speeds (E). *, p

    Journal: bioRxiv

    Article Title: Investigation of fascin1, a marker of mature dendritic cells, reveals a New role for IL-6 signaling in chemotaxis

    doi: 10.1101/2020.03.19.979104

    Figure Lengend Snippet: IL-6 signaling is required for directional migration of BMDCs. A, Analyses of migration tracks. Live cell imaging of BMDCs migrating in a collagen gel toward CCL19 was performed to obtain migration tracks (a, c e) and rose diagram plots for directionality(b, d f). a b, WT (n=19): c d, fascin1 KO (n=23): e f, WT BMDCs in the presence of a neutralizing antibody against IL-6Rα (n=28). WT BMDCs (a b) showed more consistent migration toward CCL19 than fascin1 KO counterparts (c d). Blockage of IL-6 signaling inhibits directed migration of WT BMDCs (e f). Arrows, the direction of a CCL19 gradient. B-E, Box plot analyses of parallel (B, FMI∥) and perpendicular (C, FMI⊥) forward migration indexes, directness (D), and migration speeds (E). *, p

    Article Snippet: To restore IL-6 signaling of IL-6Rα KO DCs, human IL-6 (15ng/ml, 206-IL, R & D Systems) and soluble IL-6Rα (sIL-6Rα, 25ng/ml, 227-SR, R & D Systems) were added 1hr after LPS addition.

    Techniques: Migration, Live Cell Imaging

    Impaired chemotaxis of IL-6Rα KO BMDCs and its restoration by the addition of soluble IL-6Rα (sIL-6Rα) and IL-6. BMDCs were isolated from heterozygous (hetero) and IL-6Rα KO mice. A , Immunofluorescence of IL-6Rα in heterozygous (a) and IL-6Rα KO BMDCs (b) confirming the lack of IL-6Rα in KO BMDCs. B , Collagen-coated, modified Boyden chamber chemotaxis assays of heterozygous (hetero), IL-6Rα KO, and IL-6Rα KO in the presence of sIL-6Rα and IL-6 (KO+sIL-6R/IL-6). Note that KO BMDCs show reduced chemotaxis, which was rescued by the addition of soluble IL-6Rα (sIL-6Rα) and IL-6. C D , IL-6Rα KO did not alter surface expression of CCR7. Immunofluorescence (C) showing similar levels of surface CCR7 in heterozygous (a) and IL-6Rα KO BMDCs (b). Flow cytometry analyses (D) confirming that IL-6Rα KO did not affect the levels of CCR7 surface expression. After CD86-positive heterozygous (a) and IL-6Rα KO (b) BMDCs were gated, the levels of CCR7 surface expression were examined in histogram (c).

    Journal: bioRxiv

    Article Title: Investigation of fascin1, a marker of mature dendritic cells, reveals a New role for IL-6 signaling in chemotaxis

    doi: 10.1101/2020.03.19.979104

    Figure Lengend Snippet: Impaired chemotaxis of IL-6Rα KO BMDCs and its restoration by the addition of soluble IL-6Rα (sIL-6Rα) and IL-6. BMDCs were isolated from heterozygous (hetero) and IL-6Rα KO mice. A , Immunofluorescence of IL-6Rα in heterozygous (a) and IL-6Rα KO BMDCs (b) confirming the lack of IL-6Rα in KO BMDCs. B , Collagen-coated, modified Boyden chamber chemotaxis assays of heterozygous (hetero), IL-6Rα KO, and IL-6Rα KO in the presence of sIL-6Rα and IL-6 (KO+sIL-6R/IL-6). Note that KO BMDCs show reduced chemotaxis, which was rescued by the addition of soluble IL-6Rα (sIL-6Rα) and IL-6. C D , IL-6Rα KO did not alter surface expression of CCR7. Immunofluorescence (C) showing similar levels of surface CCR7 in heterozygous (a) and IL-6Rα KO BMDCs (b). Flow cytometry analyses (D) confirming that IL-6Rα KO did not affect the levels of CCR7 surface expression. After CD86-positive heterozygous (a) and IL-6Rα KO (b) BMDCs were gated, the levels of CCR7 surface expression were examined in histogram (c).

    Article Snippet: To restore IL-6 signaling of IL-6Rα KO DCs, human IL-6 (15ng/ml, 206-IL, R & D Systems) and soluble IL-6Rα (sIL-6Rα, 25ng/ml, 227-SR, R & D Systems) were added 1hr after LPS addition.

    Techniques: Chemotaxis Assay, Isolation, Mouse Assay, Immunofluorescence, Modification, Expressing, Flow Cytometry