solubilization solution promega Search Results


solubilization solution  (Promega)
90
Promega solubilization solution
Solubilization Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solubilization solution/stop mix  (Promega)
90
Promega solubilization solution/stop mix
Solubilization Solution/Stop Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtt solubilizing solution  (Promega)
90
Promega mtt solubilizing solution
Mtt Solubilizing Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solubilization solution/stop mix g401a  (Promega)
90
Promega solubilization solution/stop mix g401a
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Solubilization Solution/Stop Mix G401a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stop solubilization solution  (Promega)
90
Promega stop solubilization solution
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Stop Solubilization Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stop solubilization solution - by Bioz Stars, 2026-08
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tmb-solubilized substrate solution tmb one  (Promega)
90
Promega tmb-solubilized substrate solution tmb one
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Tmb Solubilized Substrate Solution Tmb One, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protein-bound dye solubilized in tris base solution  (Promega)
90
Promega protein-bound dye solubilized in tris base solution
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Protein Bound Dye Solubilized In Tris Base Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tmb-solubilized substrate solution (tmbone ® 3, 3′, 5, 5′, tetramethylbenzidine)  (Promega)
90
Promega tmb-solubilized substrate solution (tmbone ® 3, 3′, 5, 5′, tetramethylbenzidine)
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Tmb Solubilized Substrate Solution (Tmbone ® 3, 3′, 5, 5′, Tetramethylbenzidine), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tmb-solubilized substrate solution (tmbone ® 3, 3′, 5, 5′, tetramethylbenzidine) - by Bioz Stars, 2026-08
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dye and solubilization solutions promega proliferation assay  (Promega)
90
Promega dye and solubilization solutions promega proliferation assay
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Dye And Solubilization Solutions Promega Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gel solubilization solution  (Promega)
90
Promega gel solubilization solution
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Gel Solubilization Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solubilization/stop solution promega 2016-04-21  (Promega)
90
Promega solubilization/stop solution promega 2016-04-21
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Solubilization/Stop Solution Promega 2016 04 21, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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solubilization solution/stop mix partg401a  (Promega)
90
Promega solubilization solution/stop mix partg401a
<t>TNF-α</t> and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells <t>(HAOEC).</t> ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .
Solubilization Solution/Stop Mix Partg401a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


TNF-α and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells (HAOEC). ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .

Journal: Scientific Reports

Article Title: VE-cadherin shedding in vitro and in patients with aortic aneurysm and dissection

doi: 10.1038/s41598-024-77940-3

Figure Lengend Snippet: TNF-α and ADAM10/17 trigger shedding of VE-cadherin (VEC) in human, aortic endothelial cells (HAOEC). ( a ) soluble VEC (sVEC) levels detected by ELISA in culture supernatants of HAOEC stimulated with various concentrations (for 4 h). ( b ) Relative sVEC levels in the culture supernatant of HAOEC treated with TNF-α (100 ng/mL) for the indicated times. Absorbance was normalized to the absorbance measured in untreated (ctrl) cells (dashed line) c , d ) sVEC levels read out by ELISA ( c ) or Western Blotting ( d ) against VEC in supernatants of HAOEC stimulated with TNF-α (100 ng/mL, 2 h) in the presence or absence of pharmacological inhibitors for ADAM10 and ADAM17 (each 10 µM). d (lower panel) densitometric quantification of 90 kDa fragment of VEC. The uncropped blot is shown in the Supplementary Fig. . e , f Diffusion of Alexa488-conjugated Dextran (10 kDa) through a confluent monolayer of HAOEC treated for 4 h with TNF-a ( e ) or ADAM10 inhibitor ( f ). A minimum of N = 3 independent experiments were conducted. P values were determined using one-way analysis of variance ( ANOVA ) ( a-d ) and unpaired t-Test ( Welch’s t-test ) for e , f .

Article Snippet: Viability of HAOEC upon treatment with various TNF-α concentrations ranging from 100 to 1000 ng/ml was performed by an commercially available “MTT” assay (Promega, G4000, including Solubilization Solution/Stop Mix G401A and Dye Solution G402A) as described by Guo et al. .

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Diffusion-based Assay