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Image Search Results
Journal: Cancer Science
Article Title: Impact of tumor‐associated macrophages on invasive ductal carcinoma of the pancreas head
doi: 10.1111/j.1349-7006.2012.02411.x
Figure Lengend Snippet: Objective measurement of the area ratio of immunopositive cells. (a) Using the section showing the maximum diameter of the invasive ductal carcinoma tumor that was stained with anti‐CD204, hot spots in the center and the periphery of the tumor were observed at a magnification of ×40. Center (b) and periphery (c) of invasive ductal carcinoma of the pancreas (magnification, ×400). We measured the area of immunopositive cell bodies at this magnification using the Automeasure function of Axio Vision 4.7.1. Axio Vision software visualized the CD204‐positive area as red‐colored areas (d) and objectively calculated the positive area ratio (summed area of immunopositive cells/measured area). CD204 expression in chronic pancreatitis (CP) tumors was measured using the section with the maximum diameter of the CP tumor (e–g). In the entire image of the CD204‐stained CP section (E; magnification, ×40), hot spots of CD204 expression at the central and the peripheral sites of the CP (f,g; magnification ×400) were selected. The positive area ratio of CD204 in the selected image was objectively calculated with Axio Vision software.
Article Snippet: The Automeasure function in
Techniques: Staining, Software, Expressing