soft agar plug Search Results


soft agar plug  (Eppendorf AG)
96
Eppendorf AG soft agar plug
Soft Agar Plug, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+agar+plug/Plug/bio_rxiv__2025__11__01__684647-43-2-27
Average 96 stars, based on 1 article reviews
soft agar plug - by Bioz Stars, 2026-10
96/100 stars
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carboxyphenol ba  (Chem Impex International)
95
Chem Impex International carboxyphenol ba
Carboxyphenol Ba, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+agar+plug/Salicylic+acid/pm33543929-33-0-5
Average 95 stars, based on 1 article reviews
carboxyphenol ba - by Bioz Stars, 2026-10
95/100 stars
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nutrient agar  (Thermo Fisher)
99
Thermo Fisher nutrient agar
Nutrient Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+agar+plug/AGAR/10__1128_slash_jb__00305___07-82-21-26
Average 99 stars, based on 1 article reviews
nutrient agar - by Bioz Stars, 2026-10
99/100 stars
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lb soft agar  (Thermo Fisher)
99
Thermo Fisher lb soft agar
Lb Soft Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+agar+plug/LB+AGAR/pmc07001489-90-12-22
Average 99 stars, based on 1 article reviews
lb soft agar - by Bioz Stars, 2026-10
99/100 stars
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sucrose recovery soft agar pad  (Thermo Fisher)
99
Thermo Fisher sucrose recovery soft agar pad
Shape <t>recovery</t> of LI spheroplasts lacking different envelope stress responses. Spheroplasts were plated on <t>soft</t> <t>agar</t> overlays, and their development was monitored by time-lapse microscopy; phase-contrast images are shown. (A) E. coli DR2 (ΔCpxR). (B) E. coli DR3 (ΔOmpR). (C) E. coli DR1 (ΔRcsB). (D) E. coli DR4 (ΔRcsC). (E) E. coli DR5 (ΔRcsF). (F) E. coli DR5C (E. coli DR5 plus plasmid pRcsF, expressing wild-type rcsF). (G) E. coli DR6 (ΔRcsA). The time after plating is displayed in the upper right corner of each panel (hours:minutes). The scale bar represents 10 μm. The phase-contrast images in panels C and E were extracted from Movies M2 and M3 in the supplemental material, respectively. See also Fig. S3.
Sucrose Recovery Soft Agar Pad, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+agar+plug/Sucrose/pmc03676047-68-7-27
Average 99 stars, based on 1 article reviews
sucrose recovery soft agar pad - by Bioz Stars, 2026-10
99/100 stars
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blood agar plates  (Chem Impex International)
95
Chem Impex International blood agar plates
Shape <t>recovery</t> of LI spheroplasts lacking different envelope stress responses. Spheroplasts were plated on <t>soft</t> <t>agar</t> overlays, and their development was monitored by time-lapse microscopy; phase-contrast images are shown. (A) E. coli DR2 (ΔCpxR). (B) E. coli DR3 (ΔOmpR). (C) E. coli DR1 (ΔRcsB). (D) E. coli DR4 (ΔRcsC). (E) E. coli DR5 (ΔRcsF). (F) E. coli DR5C (E. coli DR5 plus plasmid pRcsF, expressing wild-type rcsF). (G) E. coli DR6 (ΔRcsA). The time after plating is displayed in the upper right corner of each panel (hours:minutes). The scale bar represents 10 μm. The phase-contrast images in panels C and E were extracted from Movies M2 and M3 in the supplemental material, respectively. See also Fig. S3.
Blood Agar Plates, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/soft+agar+plug/Sodium+fluoride/pm40127571-75-13-19
Average 95 stars, based on 1 article reviews
blood agar plates - by Bioz Stars, 2026-10
95/100 stars
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Image Search Results


Shape recovery of LI spheroplasts lacking different envelope stress responses. Spheroplasts were plated on soft agar overlays, and their development was monitored by time-lapse microscopy; phase-contrast images are shown. (A) E. coli DR2 (ΔCpxR). (B) E. coli DR3 (ΔOmpR). (C) E. coli DR1 (ΔRcsB). (D) E. coli DR4 (ΔRcsC). (E) E. coli DR5 (ΔRcsF). (F) E. coli DR5C (E. coli DR5 plus plasmid pRcsF, expressing wild-type rcsF). (G) E. coli DR6 (ΔRcsA). The time after plating is displayed in the upper right corner of each panel (hours:minutes). The scale bar represents 10 μm. The phase-contrast images in panels C and E were extracted from Movies M2 and M3 in the supplemental material, respectively. See also Fig. S3.

Journal: Journal of Bacteriology

Article Title: The Rcs Stress Response and Accessory Envelope Proteins Are Required for De Novo Generation of Cell Shape in Escherichia coli

doi: 10.1128/JB.00160-13

Figure Lengend Snippet: Shape recovery of LI spheroplasts lacking different envelope stress responses. Spheroplasts were plated on soft agar overlays, and their development was monitored by time-lapse microscopy; phase-contrast images are shown. (A) E. coli DR2 (ΔCpxR). (B) E. coli DR3 (ΔOmpR). (C) E. coli DR1 (ΔRcsB). (D) E. coli DR4 (ΔRcsC). (E) E. coli DR5 (ΔRcsF). (F) E. coli DR5C (E. coli DR5 plus plasmid pRcsF, expressing wild-type rcsF). (G) E. coli DR6 (ΔRcsA). The time after plating is displayed in the upper right corner of each panel (hours:minutes). The scale bar represents 10 μm. The phase-contrast images in panels C and E were extracted from Movies M2 and M3 in the supplemental material, respectively. See also Fig. S3.

Article Snippet: Cells (2 μl) were placed onto a sucrose recovery soft agar pad (sucrose recovery medium plus 0.7% agar) solidified in the chambers of a Lab-Tek chamber slide (Nalge Nunc International, Rochester, NY.

Techniques: Time-lapse Microscopy, Plasmid Preparation, Expressing

PBP1B and LpoB are essential for the regeneration of rod-shaped cells from LI spheroplasts. (A to D) Spheroplasts were prepared from E. coli mutants and plated on sucrose soft agar overlays, and their development was monitored by time-lapse microscopy; phase-contrast images are shown. (A) E. coli DR7 (ΔPBP1B). (B) E. coli DR7C (DR7 containing plasmid pSK12, producing wild-type PBP1B). (C) E. coli DR8 (ΔLpoB). (D) E. coli DR8C (DR8 containing plasmid pLpoB, producing wild-type LpoB). (E and F) Periplasmic sfGFP (encoded by plasmid pDKR2) was produced by adding 100 μM isopropyl-β-d-thiogalactopyranoside (IPTG) to cells and incubating them for 1 h to induce gene expression, after which the cells were converted into spheroplasts and incubated for 2 h on a soft agar overlay medium containing IPTG. (E) E. coli DR7PG [ΔmrcB/dsbA(SS)-sfgfp]. (F) E. coli DR8PG [ΔlpoB/dsbA(SS)-sfgfp]. Note the presence of peripheral vesicles in the large spheroids derived from stains lacking PBP1B (A and E) and LpoB (C and F). The time after plating is displayed in the upper right corner of each panel (hours:minutes). The scale bar represents 10 μm. The phase-contrast images in panels A and C were extracted from Movies M4 and M5 in the supplemental material, respectively. See also Fig. S3.

Journal: Journal of Bacteriology

Article Title: The Rcs Stress Response and Accessory Envelope Proteins Are Required for De Novo Generation of Cell Shape in Escherichia coli

doi: 10.1128/JB.00160-13

Figure Lengend Snippet: PBP1B and LpoB are essential for the regeneration of rod-shaped cells from LI spheroplasts. (A to D) Spheroplasts were prepared from E. coli mutants and plated on sucrose soft agar overlays, and their development was monitored by time-lapse microscopy; phase-contrast images are shown. (A) E. coli DR7 (ΔPBP1B). (B) E. coli DR7C (DR7 containing plasmid pSK12, producing wild-type PBP1B). (C) E. coli DR8 (ΔLpoB). (D) E. coli DR8C (DR8 containing plasmid pLpoB, producing wild-type LpoB). (E and F) Periplasmic sfGFP (encoded by plasmid pDKR2) was produced by adding 100 μM isopropyl-β-d-thiogalactopyranoside (IPTG) to cells and incubating them for 1 h to induce gene expression, after which the cells were converted into spheroplasts and incubated for 2 h on a soft agar overlay medium containing IPTG. (E) E. coli DR7PG [ΔmrcB/dsbA(SS)-sfgfp]. (F) E. coli DR8PG [ΔlpoB/dsbA(SS)-sfgfp]. Note the presence of peripheral vesicles in the large spheroids derived from stains lacking PBP1B (A and E) and LpoB (C and F). The time after plating is displayed in the upper right corner of each panel (hours:minutes). The scale bar represents 10 μm. The phase-contrast images in panels A and C were extracted from Movies M4 and M5 in the supplemental material, respectively. See also Fig. S3.

Article Snippet: Cells (2 μl) were placed onto a sucrose recovery soft agar pad (sucrose recovery medium plus 0.7% agar) solidified in the chambers of a Lab-Tek chamber slide (Nalge Nunc International, Rochester, NY.

Techniques: Time-lapse Microscopy, Plasmid Preparation, Produced, Gene Expression, Incubation, Derivative Assay

Lpp, PBP5, and PBP6 affect the trajectory by which LI spheroplasts regenerate rod-shaped cells. LI spheroplasts were prepared from E. coli mutants and placed on sucrose soft agar overlays, and their development was monitored by time-lapse microscopy; phase-contrast images are shown. (A) E. coli DR11 (ΔLpp). (B) E. coli AV14-1 (ΔPBP4). (C) E. coli AV15-1 (ΔPBP7). (D) E. coli AV21-1 (ΔPBP5). (E) E. coli AV76-1 (ΔPBP6). The time after plating is displayed in the upper right corner of each panel (hours:minutes). The scale bar represents 10 μm.

Journal: Journal of Bacteriology

Article Title: The Rcs Stress Response and Accessory Envelope Proteins Are Required for De Novo Generation of Cell Shape in Escherichia coli

doi: 10.1128/JB.00160-13

Figure Lengend Snippet: Lpp, PBP5, and PBP6 affect the trajectory by which LI spheroplasts regenerate rod-shaped cells. LI spheroplasts were prepared from E. coli mutants and placed on sucrose soft agar overlays, and their development was monitored by time-lapse microscopy; phase-contrast images are shown. (A) E. coli DR11 (ΔLpp). (B) E. coli AV14-1 (ΔPBP4). (C) E. coli AV15-1 (ΔPBP7). (D) E. coli AV21-1 (ΔPBP5). (E) E. coli AV76-1 (ΔPBP6). The time after plating is displayed in the upper right corner of each panel (hours:minutes). The scale bar represents 10 μm.

Article Snippet: Cells (2 μl) were placed onto a sucrose recovery soft agar pad (sucrose recovery medium plus 0.7% agar) solidified in the chambers of a Lab-Tek chamber slide (Nalge Nunc International, Rochester, NY.

Techniques: Time-lapse Microscopy