Journal: Experimental and therapeutic medicine

Article Title: 1,25(OH) 2 D 3 ameliorates doxorubicin‑induced cardiomyopathy by inhibiting the NLRP3 inflammasome and oxidative stress.

doi: 10.3892/etm.2023.12112

Figure Lengend Snippet: Figure 3. Increased oxidative stress and NLRP3 inflammasome pathways are ameliorated by 1,25(OH)2D3 treatment in DOX‑induced cardiomyopathy in mice. (A) AC16 cells treated with different concentrations of 1,25(OH)2D3 (0, 25, 50, 100, 200 and 400 nM) for 24 h. Cell viability was measured using CCK‑8 assay (n=3). (B) AC16 cells were treated with 1 µM DOX and different concentrations of 1,25(OH)2D3 (0, 25, 50, 100 and 200 nM) for 24 h. Cell viability was measured using CCK‑8 assay (n=3). ****P<0.0001 vs. Control group; #P<0.05 and ####P<0.0001 vs. DOX‑only group; &&&P<0.001 vs. DOX + 25 nM 1,25(OH)2D3‑treated group; @@P<0.01 vs. DOX + 50 nM 1,25(OH)2D3‑treated group. (C) Levels of reactive oxygen species and (D) densitometric analysis in AC16 cells after treatment with DOX or DOX + 1,25(OH)2D3 via DCFH‑DA staining (n=5). *P<0.05 and ****P<0.0001 vs. Control; ###P<0.001 vs. DOX group. Levels of (E) MDA, (F) T‑SOD and (G) GSH‑Px in cardiac tissue 4 weeks after the first injection of DOX (n=5). (H) Western blotting images of cardiac tissue extracts analyzing protein expression of SOD1, SOD2, Nrf2 and KEAP1. GAPDH was used as the loading control. (I) Semi‑quantification of protein expression levels of SOD1, SOD2, Nrf2 and KEAP1. Protein expression levels relative to the Sham group were assessed by densitometric analysis (n=3). (J) Western blotting images of cardiac tissue extracts analyzing protein expression of NLRP3, ASC and Caspase‑1. GAPDH was used as the loading control. (K) Semi‑quantification of protein expression levels NLRP3, ASC and Caspase‑1. Protein expression levels relative to the Sham group were assessed by densi‑ tometric analysis (n=3). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001 vs. Sham; #P<0.05, ##P<0.01, ###P<0.001 and ####P<0.001 vs. DOX. DOX, doxorubicin hydrochloride; CCK‑8, Cell Counting Kit‑8; ns, no significant difference; DCFH‑DA, 2',7'‑dichlorofluorescin diacetate; MDA, malondialdehyde; T, total; GSH‑Px, glutathione peroxidase; SOD, superoxide dismutase; Nrf2, nuclear erythroid 2‑related factor 2; KEAP1, Kelch‑like ECH‑associated protein 1; ASC, caspase recruitment domain; NLRP3, nod‑like receptor family pyrin domain‑containing 3 inflammasome.

Article Snippet: The dilutions of primary antibodies used were as follows: Collagen I (cat. No. GTX26308; 1:1,000; GeneTex, Inc.), α‐SMA (α‐smooth muscle actin; cat. No. ab5694; 1:1,000; Abcam), IL‐1β (cat. no. ab9722; 0.25 μg/ml; Abcam), IL‐6 (cat. No. A0286; 1:1,000; Abclonal Biotech Co., Ltd.), TNF‐α (cat. No. GTX110520; 1:500; GeneTex, Inc.), SOD1 (cat. No. ab16831; 1:2,000; Abcam), SOD2 (cat. No. NB100‐1992; 1:2,000; Novus Biologicals, LLC), Nrf2 (cat. No. GTX135165; 1:1,000; GeneTex, Inc.), KEAP1 (Kelch‐like ECH‐associated protein 1; cat. No. ab119403; 1:1,000; Abcam), Caspase‐1 (cat. No. 83383; 1:500; Cell Signaling Technology, Inc.), NLRP3 (cat. No. ab214185; 1:1,000; Abcam), ASC (caspase recruitment domain; cat. No. GTX55818; 1:1,000; GeneTex, Inc.) and GAPDH (cat. No. 2118; 1:1,000; Cell Signaling Technology, Inc.).

Techniques: CCK-8 Assay, Control, Staining, Injection, Western Blot, Expressing

Journal: BMC Neuroscience

Article Title: Sex-specific cypermethrin-induced hippocampal neurotoxicity is associated with alterations in signaling molecules for antioxidant defense, membrane integrity, apoptosis, and GABAergic integrity

doi: 10.1186/s12868-025-00988-y

Figure Lengend Snippet: GnRH ( A ), Na+/K + ATPase ( B ), PGE-2 ( C ), and COX-2 concentrations in the brains of Male and Female Wistar rats exposed to corn oil (control), low dose (6.25 mg/kg), and high dose (12.5 mg/kg) of cypermethrin, respectively. Asterisk (*) indicates significant ( P < 0.05) weight loss. Bars are mean ± SD

Article Snippet: The following kits were used: GnRH (Sandwich ELISA, Kit #MBS269200, MyBioSource), Na+/K+-ATPase (Colorimetric, Kit #E-BC-K022-M, Elabscience), PGE2 (EIA Kit #ADI-900-001, Enzo Life Sciences), COX-2 (ELISA, Kit #MBS7254655, MyBioSource).

Techniques: Control