Journal:

Article Title: PCR Detection, Characterization, and Distribution of Virulence Genes in Aeromonas spp.

doi:

Figure Lengend Snippet: Results of PCR and characteristics of the 232-bp amplicons of reference strains

Article Snippet: Lanes: 1 and 15, DNA size marker VIII (Roche Diagnostics AG; catalog no. 1336 045); 2, a PCR-RFLP 2 of A17 (ATCC 7966), a reference strain of HG1 isolated from canned milk from the United States; 3, PCR-RFLP 2 of A307 (ATCC 51108), a reference strain of HG2 isolated from a fish in France; 4, PCR-RFLP 2 of A14 (LMG 13674), a reference strain of HG3 that is an environmental isolate from Germany; 5, PCR-RFLP type 1 of A914 (CDC 9179), a reference strain of HG6 (source unknown); 6, PCR-RFLP 1 of A312 (ATCC 43979), a reference strain of HG7 that is a fish isolate from France; 7, PCR-RFLP 1 of A10 (CCUG 30356), a reference strain of HG8 that is an environmental isolate from Germany; 8, PCR-RFLP 1 of A901 (ATCC 35624), a reference strain of HG10 that is a clinical isolate (sputum) from the United States; 9, PCR-RFLP 1 of A926 (CDC 3136), a reference strain of HG11 that is a water isolate from the United States; 10, PCR-RFLP 3 of A1645 (ATCC 49659), a reference strain of HG13 that is a clinical isolate from the United States; 11, uncut 232-bp amplicon of strain A1645; 12, PCR-RFLP 2 of a wild-type strain (BP 3) of HG1 that is a clinical isolate of HG1 from Bangladesh; 13, PCR-RFLP 2 of a wild-type strain (CBK 45) of HG3 that is a pork meat isolate from Switzerland; 14, PCR-RFLP 1 of a wild-type strain (BE 6) of HG8 that is an environmental isolate from Bangladesh.

Techniques: Fluorescence In Situ Hybridization

Journal:

Article Title: PCR Detection, Characterization, and Distribution of Virulence Genes in Aeromonas spp.

doi:

Figure Lengend Snippet: PCR amplicon of 232 bp and PCR-RFLP types of 232-bp amplicons of different HGs of reference strains of Aeromonas spp. PCR amplicon and PCR-RFLP types of reference and wild-type strains of Aeromonas spp. on 4% Metaphor agarose gel (Bioconcept, Allschwil, Switzerland) stained with ethidium bromide at 0.5 μg/ml (Bio-Rad). Lanes: 1 and 15, DNA size marker VIII (Roche Diagnostics AG; catalog no. 1336 045); 2, a PCR-RFLP 2 of A17 (ATCC 7966), a reference strain of HG1 isolated from canned milk from the United States; 3, PCR-RFLP 2 of A307 (ATCC 51108), a reference strain of HG2 isolated from a fish in France; 4, PCR-RFLP 2 of A14 (LMG 13674), a reference strain of HG3 that is an environmental isolate from Germany; 5, PCR-RFLP type 1 of A914 (CDC 9179), a reference strain of HG6 (source unknown); 6, PCR-RFLP 1 of A312 (ATCC 43979), a reference strain of HG7 that is a fish isolate from France; 7, PCR-RFLP 1 of A10 (CCUG 30356), a reference strain of HG8 that is an environmental isolate from Germany; 8, PCR-RFLP 1 of A901 (ATCC 35624), a reference strain of HG10 that is a clinical isolate (sputum) from the United States; 9, PCR-RFLP 1 of A926 (CDC 3136), a reference strain of HG11 that is a water isolate from the United States; 10, PCR-RFLP 3 of A1645 (ATCC 49659), a reference strain of HG13 that is a clinical isolate from the United States; 11, uncut 232-bp amplicon of strain A1645; 12, PCR-RFLP 2 of a wild-type strain (BP 3) of HG1 that is a clinical isolate of HG1 from Bangladesh; 13, PCR-RFLP 2 of a wild-type strain (CBK 45) of HG3 that is a pork meat isolate from Switzerland; 14, PCR-RFLP 1 of a wild-type strain (BE 6) of HG8 that is an environmental isolate from Bangladesh.

Article Snippet: Lanes: 1 and 15, DNA size marker VIII (Roche Diagnostics AG; catalog no. 1336 045); 2, a PCR-RFLP 2 of A17 (ATCC 7966), a reference strain of HG1 isolated from canned milk from the United States; 3, PCR-RFLP 2 of A307 (ATCC 51108), a reference strain of HG2 isolated from a fish in France; 4, PCR-RFLP 2 of A14 (LMG 13674), a reference strain of HG3 that is an environmental isolate from Germany; 5, PCR-RFLP type 1 of A914 (CDC 9179), a reference strain of HG6 (source unknown); 6, PCR-RFLP 1 of A312 (ATCC 43979), a reference strain of HG7 that is a fish isolate from France; 7, PCR-RFLP 1 of A10 (CCUG 30356), a reference strain of HG8 that is an environmental isolate from Germany; 8, PCR-RFLP 1 of A901 (ATCC 35624), a reference strain of HG10 that is a clinical isolate (sputum) from the United States; 9, PCR-RFLP 1 of A926 (CDC 3136), a reference strain of HG11 that is a water isolate from the United States; 10, PCR-RFLP 3 of A1645 (ATCC 49659), a reference strain of HG13 that is a clinical isolate from the United States; 11, uncut 232-bp amplicon of strain A1645; 12, PCR-RFLP 2 of a wild-type strain (BP 3) of HG1 that is a clinical isolate of HG1 from Bangladesh; 13, PCR-RFLP 2 of a wild-type strain (CBK 45) of HG3 that is a pork meat isolate from Switzerland; 14, PCR-RFLP 1 of a wild-type strain (BE 6) of HG8 that is an environmental isolate from Bangladesh.

Techniques: Amplification, Agarose Gel Electrophoresis, Staining, Marker, Isolation

Journal: mBio

Article Title: Bioinformatic Genome Comparisons for Taxonomic and Phylogenetic Assignments Using Aeromonas as a Test Case

doi: 10.1128/mBio.02136-14

Figure Lengend Snippet: General features of the Aeromonas genomes

Article Snippet: A. veronii bv. sobria , AER 39 , 4.42 , 4 , 283 , 1,516,045 , 58.9 , 3,948 , AGWT01 , The Broad Institute.

Techniques:

Journal: mBio

Article Title: Bioinformatic Genome Comparisons for Taxonomic and Phylogenetic Assignments Using Aeromonas as a Test Case

doi: 10.1128/mBio.02136-14

Figure Lengend Snippet: (A) Maximum likelihood reconstruction of 16 single-copy housekeeping genes. Support values are represented by dots: red (90%+ bootstraps), orange (80%+), yellow (70%+). (B) Approximate maximum likelihood reconstruction of 2,710 orthologous groups found in 90% or more of the taxa. aLRT SH-like support values equal to or greater than 0.97 are represented by red dots. The species A . veronii , A . hydrophila , A . dhakensis , A . salmonicida , and A . caviae are color-coded in both trees. Additionally, two previously misidentified taxa, A . veronii AMC 34 and A . hydrophila AH4, are shown in red and teal, respectively. Eight well-supported clades were shared between the two reconstructions. They are shown by the colored bars and are numbered 1 through 8.

Article Snippet: A. veronii bv. sobria , AER 39 , 4.42 , 4 , 283 , 1,516,045 , 58.9 , 3,948 , AGWT01 , The Broad Institute.

Techniques:

Journal: Pathogens

Article Title: Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

doi: 10.3390/pathogens13060432

Figure Lengend Snippet: List of the certified Aeromonas strains used in the present study to implement SARAMIS software version V4.12.

Article Snippet: A. sobria , NCIMB 75 , Diseased freshwater fish.

Techniques: Software, Infection

Journal: Pathogens

Article Title: Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

doi: 10.3390/pathogens13060432

Figure Lengend Snippet: Reference Spectra and Super Spectra list of Aeromonas species included in SARAMIS Database V4.12.

Article Snippet: A. sobria , NCIMB 75 , Diseased freshwater fish.

Techniques:

Journal: Pathogens

Article Title: Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

doi: 10.3390/pathogens13060432

Figure Lengend Snippet: Identification results of different Aeromonas strains processed using MALDI-TOF technology with identification software SARAMIS, version 4.12.

Article Snippet: A. sobria , NCIMB 75 , Diseased freshwater fish.

Techniques: Software

Journal: Pathogens

Article Title: Use of Matrix-Assisted and Laser Desorption/Ionization Time-of-Flight Technology in the Identification of Aeromonas Strains Isolated from Retail Sushi and Sashimi

doi: 10.3390/pathogens13060432

Figure Lengend Snippet: Comparison between the identification of different Aeromonas strains using SARAMIS software V 4.12 and an implemented version of the software (V4.12 plus ) proposed in the present study. The best percentages of strain identifications performed with both versions of the software are reported regardless of the type of growth medium used.

Article Snippet: A. sobria , NCIMB 75 , Diseased freshwater fish.

Techniques: Comparison, Software