Journal: Oncology Letters

Article Title: Annexin A7 expression is downregulated in late-stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate

doi: 10.3892/ol.2018.8576

Figure Lengend Snippet: Expression of Annexin A7 in differently differentiated gastric cancer tissues. (A) The expression of Annexin A7 mRNA was measured by fluorescence reverse transcription-quantitative polymerase chain reaction in differently differentiated gastric cancer tissues. (B) The Annexin A7 protein levels in differently differentiated gastric cancer tissues were determined by western blot analysis, with GAPDH as the loading control. Left, representative western blots and right, integrated optical density values of the western blot results. Highly, highly differentiated gastric adenocarcinoma; Moderately, moderately differentiated gastric adenocarcinoma; Poorly, poorly differentiated gastric adenocarcinoma. *P<0.05 compared with the Highly group; # P<0.05 compared with the Moderately group.

Article Snippet: The membranes were incubated with a rabbit anti-human Annexin A7 primary antibody (dilution, 1:1,000; 10154-2-AP; ProteinTech Group, Inc., Chicago, IL, USA) or a rabbit anti-human GAPDH primary antibody (dilution, 1:3,000; YT5052; ImmunoWay Biotechnology, Plano, TX, USA) overnight at 4°C, followed by washing with TBS containing 0.1% Tween-20 and incubation with an IRDye 680LT-conjugated secondary antibody (dilution, 1:1,000; Goat anti-Rabbit, A11369; Invitrogen; Thermo Fisher Scientific, Inc.) at room temperature in the dark for 2 h. An Odyssey double color infrared laser imaging system (Odyssey Sa Software CD Version 2.0; LI-COR Biosciences, Lincoln, NE, USA) was adopted for scanning and calculating the integral absorbance values of the bands, and the ratio of the gray values of the target band and the corresponding GAPDH band was used to determine the target protein expression.

Techniques: Expressing, Fluorescence, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: Oncology Letters

Article Title: Annexin A7 expression is downregulated in late-stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate

doi: 10.3892/ol.2018.8576

Figure Lengend Snippet: Expression of Annexin A7 in differently differentiated gastric cancer cell lines. (A) The expression of Annexin A7 mRNA was measured by fluorescence reverse transcription-quantitative polymerase chain reaction in differently differentiated gastric cancer cell lines. (B) The Annexin A7 protein levels in differently differentiated gastric cancer cell lines were determined by western blot analysis, with GAPDH as the loading control. Left, representative western blots and right, integrated optical density values of the western blot results. *P<0.05 compared with the MKN74 cells; # P<0.05 compared with the SGC7901 cells.

Article Snippet: The membranes were incubated with a rabbit anti-human Annexin A7 primary antibody (dilution, 1:1,000; 10154-2-AP; ProteinTech Group, Inc., Chicago, IL, USA) or a rabbit anti-human GAPDH primary antibody (dilution, 1:3,000; YT5052; ImmunoWay Biotechnology, Plano, TX, USA) overnight at 4°C, followed by washing with TBS containing 0.1% Tween-20 and incubation with an IRDye 680LT-conjugated secondary antibody (dilution, 1:1,000; Goat anti-Rabbit, A11369; Invitrogen; Thermo Fisher Scientific, Inc.) at room temperature in the dark for 2 h. An Odyssey double color infrared laser imaging system (Odyssey Sa Software CD Version 2.0; LI-COR Biosciences, Lincoln, NE, USA) was adopted for scanning and calculating the integral absorbance values of the bands, and the ratio of the gray values of the target band and the corresponding GAPDH band was used to determine the target protein expression.

Techniques: Expressing, Fluorescence, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: Oncology Letters

Article Title: Annexin A7 expression is downregulated in late-stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate

doi: 10.3892/ol.2018.8576

Figure Lengend Snippet: Apoptosis was increased in late-stage gastric adenocarcinoma cell lines. (A) Apoptosis was analyzed using Annexin V-FITC/PI staining and flow cytometry in MKN74, SGC7901 and BGC823 cells. The right lower quadrant demonstrates early apoptotic cells and the right upper quadrant demonstrates late apoptotic cells. (B) Quantification of the percentage of apoptotic cells in MKN74, SGC7901 and BGC823 cells. *P<0.05 compared with the MKN74 cells; # P<0.05 compared with the SGC7901 cells. FITC, fluorescein isothiocyanate; PI, propidium iodide.

Article Snippet: The membranes were incubated with a rabbit anti-human Annexin A7 primary antibody (dilution, 1:1,000; 10154-2-AP; ProteinTech Group, Inc., Chicago, IL, USA) or a rabbit anti-human GAPDH primary antibody (dilution, 1:3,000; YT5052; ImmunoWay Biotechnology, Plano, TX, USA) overnight at 4°C, followed by washing with TBS containing 0.1% Tween-20 and incubation with an IRDye 680LT-conjugated secondary antibody (dilution, 1:1,000; Goat anti-Rabbit, A11369; Invitrogen; Thermo Fisher Scientific, Inc.) at room temperature in the dark for 2 h. An Odyssey double color infrared laser imaging system (Odyssey Sa Software CD Version 2.0; LI-COR Biosciences, Lincoln, NE, USA) was adopted for scanning and calculating the integral absorbance values of the bands, and the ratio of the gray values of the target band and the corresponding GAPDH band was used to determine the target protein expression.

Techniques: Staining, Flow Cytometry

Journal: Oncology Letters

Article Title: Annexin A7 expression is downregulated in late-stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate

doi: 10.3892/ol.2018.8576

Figure Lengend Snippet: Correlation among Annexin A7 expression, differentiation grade and apoptosis rate in gastric cancer tissues and cell lines was assessed by Spearman's rank correlation analysis.

Article Snippet: The membranes were incubated with a rabbit anti-human Annexin A7 primary antibody (dilution, 1:1,000; 10154-2-AP; ProteinTech Group, Inc., Chicago, IL, USA) or a rabbit anti-human GAPDH primary antibody (dilution, 1:3,000; YT5052; ImmunoWay Biotechnology, Plano, TX, USA) overnight at 4°C, followed by washing with TBS containing 0.1% Tween-20 and incubation with an IRDye 680LT-conjugated secondary antibody (dilution, 1:1,000; Goat anti-Rabbit, A11369; Invitrogen; Thermo Fisher Scientific, Inc.) at room temperature in the dark for 2 h. An Odyssey double color infrared laser imaging system (Odyssey Sa Software CD Version 2.0; LI-COR Biosciences, Lincoln, NE, USA) was adopted for scanning and calculating the integral absorbance values of the bands, and the ratio of the gray values of the target band and the corresponding GAPDH band was used to determine the target protein expression.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Annexin A7 expression is downregulated in late-stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate

doi: 10.3892/ol.2018.8576

Figure Lengend Snippet: Correlation among Annexin A7 protein expression, differentiation grade and apoptosis index in gastric cancer tissues was assessed by Spearman's rank correlation analysis.

Article Snippet: The membranes were incubated with a rabbit anti-human Annexin A7 primary antibody (dilution, 1:1,000; 10154-2-AP; ProteinTech Group, Inc., Chicago, IL, USA) or a rabbit anti-human GAPDH primary antibody (dilution, 1:3,000; YT5052; ImmunoWay Biotechnology, Plano, TX, USA) overnight at 4°C, followed by washing with TBS containing 0.1% Tween-20 and incubation with an IRDye 680LT-conjugated secondary antibody (dilution, 1:1,000; Goat anti-Rabbit, A11369; Invitrogen; Thermo Fisher Scientific, Inc.) at room temperature in the dark for 2 h. An Odyssey double color infrared laser imaging system (Odyssey Sa Software CD Version 2.0; LI-COR Biosciences, Lincoln, NE, USA) was adopted for scanning and calculating the integral absorbance values of the bands, and the ratio of the gray values of the target band and the corresponding GAPDH band was used to determine the target protein expression.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Annexin A7 expression is downregulated in late-stage gastric cancer and is negatively correlated with the differentiation grade and apoptosis rate

doi: 10.3892/ol.2018.8576

Figure Lengend Snippet: Correlation among Annexin A7 protein expression, differentiation grade and apoptosis rate in gastric cancer cell lines was assessed by Spearman's rank correlation analysis.

Article Snippet: The membranes were incubated with a rabbit anti-human Annexin A7 primary antibody (dilution, 1:1,000; 10154-2-AP; ProteinTech Group, Inc., Chicago, IL, USA) or a rabbit anti-human GAPDH primary antibody (dilution, 1:3,000; YT5052; ImmunoWay Biotechnology, Plano, TX, USA) overnight at 4°C, followed by washing with TBS containing 0.1% Tween-20 and incubation with an IRDye 680LT-conjugated secondary antibody (dilution, 1:1,000; Goat anti-Rabbit, A11369; Invitrogen; Thermo Fisher Scientific, Inc.) at room temperature in the dark for 2 h. An Odyssey double color infrared laser imaging system (Odyssey Sa Software CD Version 2.0; LI-COR Biosciences, Lincoln, NE, USA) was adopted for scanning and calculating the integral absorbance values of the bands, and the ratio of the gray values of the target band and the corresponding GAPDH band was used to determine the target protein expression.

Techniques: Expressing

Journal: Advanced Science

Article Title: Stage‐Specific H3K14 and H3K23 Succinylation Orchestrates Insect Metamorphosis and Oogenesis

doi: 10.1002/advs.75624

Figure Lengend Snippet: Regulation of suc‐H3 K14 and suc‐H3 K23 by the GPCR‐RTK‐PLC‐PKC cascade. (A) Effect of GPCR (Suramin), RTK (SU6668/genistein), PLC (U73122), CaMKII (KN‐93), and PKC (CCL) inhibitors on JH‐induced suc‐H3 K14 in the fourth instar nymphs. (B) Effect of Ca 2+ channel blockers (felodipine, L‐type; flunarizine, T‐type; cav2.2, N‐type; ω‐Agatoxin TK, P/Q‐type; and SNX‐482, R‐type) on suc‐H3 K14 . (C) Knockdown efficiency of PKC isoforms in the fat body of fourth instar nymphs. ** , p < 0.01; n = 3. (D) Effect of PKC isoform knockdown on suc‐H3 K14 . (E, F) Effect of GPCR, RTK, PLC, CaMKII, and PKC inhibitors ( E ) and Ca 2+ channel blockers ( F ) on JH‐induced suc‐H3 K23 in the adult females. (G) Knockdown efficiency of PKC isoforms in the adult females. ** , p < 0.01; n = 3). (H) Effect of PKC isoform knockdown on suc‐H3 K23 in the adult females.

Article Snippet: In pharmacological experiments, the early fourth instar nymphs and previtellogenic adults of L. migratoria were injected with suramin (KKL MED; 30 μg, 150 μg), SU6668 (Selleck; 0.6 μg, 3 μg), genistein (MCE; 1.2 μg, 6 μg), U73122 (MCE; 2 μg, 10 μg), KN‐93 (MCE; 0.5 μg, 10 μg), CCL (Selleck; 1 μg, 5 μg), flunarizine (Selleck; 10 μg, 50 μg), Felodipine (TOPSCIENCE; 1 μg, 5 μg), Cav2.2 blocker 1 (TOPSCIENCE; 2 μg, 10 μg), ω‐Agatoxin TK (MCE; 0.25 μg,1 μg) and SNX‐482 (MCE; 0.25 μg, 1 μg), followed by JH or DMSO treatment for 30 min.

Techniques: Knockdown